Enzyme Kinetics: Catalysis and Control

Enzyme Kinetics: Catalysis and Control

A Reference of Theory and Best-Practice Methods

1st Edition - June 16, 2010

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  • Author: Daniel Purich
  • eBook ISBN: 9780123809254
  • Hardcover ISBN: 9780123809247

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Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes.

Key Features

  • Historical analysis of kinetic principles including advanced enzyme science
  • Provides both theoretical and practical measurements tools
  • Coverage of single molecular kinetics
  • Examination of force generation mechanisms
  • Discussion of organic and inorganic enzyme reactions


Professionals and graduate students researching enzymes in biochemistry and molecular biology, biotechnology, bioengineering, plant sciences, chemical engineering, and pharmacology fields

Table of Contents


    1. An Introduction to Enzyme Rate Processes
    2. Active Sites & Their Chemical Properties
    3. Fundamentals of Chemical Kinetics
    4. Practical Aspects of Measuring Rates & Kinetic
    5. Initial-Rate Kinetics of One-Substrate Enzyme-Catalyzed Reactions
    6. Initial-Rate Kinetics of Multi-Substrate Enzyme-Catalyzed Reactions
    7. Other Factors Influencing Enzyme Activity
    8. Kinetic Behavior of Enzyme Inhibitors
    9. Isotopic Probes of Biological Catalysis
    10. Probing Fast Enzyme Processes
    11. Regulatory Behavior of Enzymes
    12. Single-Molecule Enzyme Kinetics
    13. Force Generation in Mechanoenzyme Catalysis
    14. On Keeping a Research Notebook
    15. Steady-State Rate Equations for Selected One- & Two-Substrate Enzyme-Catalyzed Reactions
    16. Summary of Enzyme Kinetic Mechanisms Abbreviations & Symbols References

Product details

  • No. of pages: 920
  • Language: English
  • Copyright: © Elsevier 2010
  • Published: June 16, 2010
  • Imprint: Elsevier
  • eBook ISBN: 9780123809254
  • Hardcover ISBN: 9780123809247

About the Author

Daniel Purich

Dr. Purich earned his Doctor of Philosophy degree in 1973 for his kinetic characterization of brain hexokinase under the preceptorship of Professor Herbert J. Fromm in the Department of Biochemistry & Biophysics at Iowa State University. As a Staff Research Fellow with Dr. Earl Stadtman at the National Institutes of Health, he conducted research on the cascade of covalent interconverting enzymes that control the glutamine synthetase reaction in Escherichia coli. Dr. Purich subsequently joined the Department of Chemistry at the University of California Santa Barbara where he rose through the ranks as Assistant Professor (1973-78), Associate Professor (1978-82), and Professor (1982-84). At UC Santa Barbara, he was awarded an Alfred P. Sloan Fellow in Chemistry (1978-80), an NIH Research Career Development Award (1978-1983), and the Campus-wide Outstanding Teacher Award (1978). In 1984, he assumed the post of Professor and Chairman of the Department of Biochemistry & Molecular Biology at the University of Florida College of Medicine, and in 1996 he retired as Chairman to resume full-time activities as a professor. Dr. Purich has served on the editorial boards of the Journal of Biological Chemistry (1981-87) and Archives of Biochemistry and Biophysics (1975-85), and as a regular member of the NIH Biochemistry Study Section. Professor Purich succeeds the late Alton Meister as series editor for Advances in Enzymology, and he continues to edit the multi-volume treatise "Enzyme Kinetics & Mechanism" appearing as volumes 63, 64, 87, and 249 in Methods in Enzymology. He has also published Contemporary Enzyme Kinetics and Mechanism (1st ed.,1983; 2nd ed.,1996) based on chapters selected from his Methods volumes.

Affiliations and Expertise

Professor of Biochemistry and Molecular Biology, University of Florida Health Science Center, FL, USA

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  • Annalisa C. Tue May 15 2018

    A must-have for professionals

    A great book for people involved in R&D on enzyme assays; be it bench tips or in-depth theory about kinetics, you won't find anywhere else what Purich was able to pour into this book. Since "Enzyme Kinetics: Catalysis and Control" saved by PhD, it might be a bit personal, but I can't recommend it enough.