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General Approaches to Biological Catalysis: C.A. Fierke and G.G. Hammes, Transient Kinetic Approaches to Enzyme Mechanisms. K.A. Johnson, Rapid Quench Kinetic Analysis of Polymerases, Adenosinetriphosphatases, and Enzyme Intermediates. R.G. Duggleby, Analysis of Enzyme Progress Curves by Nonlinear Regression. B.V. Plapp, Site-Directed Mutagenesis: A Tool for Studying Enzyme Catalysis. Inhibitors as Mechanistic Probes: H.J. Fromm, Reversible Enzyme Inhibitors as Mechanistic Probes. S.E. Szedlacsek and R.G. Duggleby, Kinetics of Slow and Tight-Binding Inhibitors. W.W. Cleland, Kinetic Method for Determination of Dissociation Constants of Metal Ion(Nucleotide Complexes. B.F.Cooper and F.B. Rudolph, Product Inhibition Applications. K.L. Rebholz and D.B. Northrop, Kinetics of Iso Mechanisms. R.B. Silverman, Mechanism-Based Enzyme Inactivators. A. Radzicka and R. Wolfenden, Transition State and Multisubstrate Analog Inhibitors. Isotopic Probles of Enzyme Action: I.A. Rose, Partition Analysis: Detecting Enzyme Reaction Cycle Intermediates. W.W. Cleland, Isotope Effects: Determination of Enzyme Transition State Structure. B.J. Bahnson and J.P. Klinman, Hydrogen Tunneling in Enzyme Catalysis. L.S. Mullins and F.M. Raushel, Positional Isotope Exchange as Probe of Enzyme Action. M.-D. Tsai, R.-T. Jiang, T. Dahnke, and Z. Shi, Manipulating Phosphorus Stereospecificity of Adenylate Kinase by Site-Directed Mutagenesis. F.C. Wedler, Equilibrium Isotope Exchange in Enzyme Catalysis. D.N. Silverman, Proton Transfer in Carbonic Anhydrase Measured by Equilibrium Isotope Exchange. Kinetics of Specialized Systems: J.D. Stewart, I. Lee, B.A. Posner, and S.J. Benkovic, Expression of Properly Folded Catalytic Antibodies in Escherichia coli. K.E. Neet, Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects. M.K. Jain, M.H. Gelb, J. Rogers, and O.G. Berg, Kinetic Basis for Interfacial Catalysis by Phospholipase A2. Author Index. Subject Index.
General Description of the Volume: This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
- Mechanisms of enzyme catalysis and inhibition
- Enzyme structure and function
- Regulatory control of enzymes
- Action of catalytic antibodies and ribozymes
Biochemists; biophysicists; molecular biologists; physical chemists; pharmacologists; analytical, organic, and medicinal chemists; biotechnologists; and graduate students in these areas.
- No. of pages:
- © Academic Press 1995
- 22nd March 1995
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Praise for the Series
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE
"Incomparably useful." --ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977–1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982–1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981–1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.
University of Florida, Gainesville, U.S.A.
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