Enzyme Engineering and Evolution: Specific Enzyme Applications

Enzyme Engineering and Evolution: Specific Enzyme Applications

1st Edition - September 15, 2020
This is the Latest Edition
  • Editor: Dan Tawfik
  • Hardcover ISBN: 9780128244319
  • eBook ISBN: 9780128244326

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Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Engineering lytic polysaccharide monooxygenases (LPMOs)

    Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink

    2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases

    Daniela M. Carminati, Eric J. Moore and Rudi Fasan

    3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries

    Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer

    4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution

    Duncan S. Macdonald and Donald Hilvert

    5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling

    Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier

    6. Engineering aldolases for asymmetric synthesis

    Mikael Widersten

    7. Ultra-high throughput screening for novel protease specificities

    Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke

    8. Directed evolution of transcriptional switches using dual-selector systems

    Yuki Kimura and Daisuke Umeno

    9. Methods for competitive enrichment and evaluation of superior DNA ligases

    Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley

    10. Directed evolution of custom polymerases using droplet microfluidics

    Derek Vallejo, Ali Nikoomanzar and John C. Chaput

    11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution

    Pu Xue, Tong Si and Huimin Zhao

    12. Split enzymes: Design principles and strategy

    Shion A. Lim and James A. Wells

Product details

  • No. of pages: 312
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: September 15, 2020
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128244319
  • eBook ISBN: 9780128244326

About the Serial Volume Editor

Dan Tawfik

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

Affiliations and Expertise

Principal Investigator, Department of Biomolecular Sciences, Weizmann Institute of Science, Israel