COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Enzyme Engineering and Evolution: Specific Enzyme Applications - 1st Edition - ISBN: 9780128244319

Enzyme Engineering and Evolution: Specific Enzyme Applications, Volume 644

1st Edition

0.0 star rating Write a review
Serial Volume Editor: Dan Tawfik
Hardcover ISBN: 9780128244319
Imprint: Academic Press
Published Date: 1st October 2020
Page Count: 412
Sales tax will be calculated at check-out Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

1. Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis
Miguel Alcalde
2. Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase
Yasuhisa Asano
3. Methods to assess correlation networks for engineering transketolase
Paul Dalby
4. Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics
Jiri Damborsky
5. Engineering lytic polysaccharide monoxygenases (LPMOs)
Vincent Eijsink
6. Emulsion-based directed evolution of enzymes in yeast.
Andrew D. Ellington
7. Generation of myoglobin-based carbene transferases with a non-native cofactor
Rudi Fasan
8. Loop remodeling for generating the novel function of enzymes
Yan Feng
9. Rieske non-heme iron dioxygenases
Bernard Hauer
10. Biocatalytic synthesis of enantioenriched aldol adducts using artificial carboligases
Donald Hilvert
11. Choosing parameters for the consensus sequence approach for stabilization of proteins
Romas Kazlauskas
12. Structure-guided engineering of the substrate specificity and catalytic activity of an enzyme
Hak-Sung Kim
13. Exploring the substrate space of transglutaminase in the frame of antibody engineering (Glutamine walking.....)
Harald Kolmar
14. Biological fitness landscapes by deep mutational scanning
Marc Ostermeier
15. Glutamine-walking: creating reactive substrates for transglutaminase-mediated protein labelling
Joelle Pelletier
16. Focused Rational Site-specific Iterative Saturation Mutagenesis (FRISM)
Qi Wu
17. Statistical epistasis analysis to elude mutational networks in the evolution of new enzyme functions
Nobuhiko Tokuriki
18. Directed evolution of stereoselective aldolases
Mikael Widersten
19. Ultra-highthroughput screening for novel protease specificities
Sven Panke
20. Directed evolution of transcriptional switches using dual selector systems
Daisuke Umeno
21. Circular permutation: a different way to engineer enzyme structure and function
Stefan Lutz
22. Methods for directed evolution and evaluation of DNA ligases
David F. Ackerley
23. Polymerase Evolution by Droplet-base Optical Polymerase Sorting (DrOPS)
John Chaput
24. The use of consensus sequence information to engineer stability and activity in proteins
Doug Barrick
25. Machine-learning-guided protein engineering
Mehdi D. Davari
26. Generation of myoglobin-based carbene transferases with a non-native cofactor
Rudi Fasan
27. Development of a Mass Spectrometry based High Throughput Screening Method for Directed Evolution of Enzymes
Huimin Zhao
28. Split enzymes: Design principles and strategy
James A. Wells
29. Ultrahigh throughput screening for protein function in droplets
Florian Hollfelder


This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


No. of pages:
© Academic Press 2020
1st October 2020
Academic Press
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editor

Dan Tawfik

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

Affiliations and Expertise

Principal Investigator, Department of Biomolecular Sciences, Weizmann Institute of Science, Israel