Enzyme Catalysis and Regulation

Enzyme Catalysis and Regulation

1st Edition - January 28, 1982

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  • Author: Gorden Hammes
  • eBook ISBN: 9780323158176

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Description

Enzyme Catalysis and Regulation is an introduction to enzyme catalysis and regulation and covers topics ranging from protein structure and dynamics to steady-state enzyme kinetics, multienzyme complexes, and membrane-bound enzymes. Case studies of selected enzyme mechanisms are also presented. This book consists of 11 chapters and begins with a brief overview of enzyme structure, followed by a discussion on methods of probing enzyme structure such as X-ray crystallography and optical spectroscopy. Kinetic methods are then described, with emphasis on the general principles of steady-state and transient kinetics. The chemical principles involved in enzyme catalysis are also discussed, and case studies of a few well-documented enzymes are presented. The regulation of enzyme activity is analyzed from a nongenetic viewpoint, with particular reference to binding isotherms and models for allosterism. Two particular enzymes, aspartate transcarbamoylase and phosphofructokinase, are used as examples of well-studied regulatory enzymes. The last two chapters focus on multienzyme complexes and membrane-bound enzymes. This monograph is intended for graduate students, advanced undergraduates, and research workers in molecular biology and biochemistry.

Table of Contents


  • Preface

    1. Protein Structure and Dynamics

    References

    General References on Proteins and Enzymes

    2. Probes of Enzyme Structure

    Introduction

    Amino Acid Sequencing

    X-Ray Crystallography

    Chemical Modification

    Optical Spectroscopy

    Nuclear Magnetic Resonance

    Spin Labels

    References

    3. Steady-State Enzyme Kinetics

    Introduction

    Single Substrate-Single Product

    King-Altman Method

    Inhibition of Enzyme Catalysis

    Multiple Substrates

    Isotope Exchange at Equilibrium

    pH Dependence of Enzyme Catalysis

    Temperature Dependence of Enzyme Catalysis

    Conclusion

    References

    4. Transient Kinetic Methods for Studying Enzymes

    Introduction

    Rapid Mixing Methods

    Chemical Relaxation Methods

    General Solutions of Kinetic Equations

    Rate Equations near Equilibrium

    References

    5. Some Chemical Aspects of Enzyme Catalysis

    Catalysis

    Acid-Base Catalysis

    Nucleophilic Catalysis

    Electrophilic Catalysis

    Stereochemistry

    Kinetic Isotope Effects

    References

    6. Elementary Steps in Enzyme Catalysis

    Introduction

    Binding of Substrates

    Conformational Changes

    Acid-Base Catalysis

    Covalent Intermediates

    Advantages of Enzymes

    References

    7· Case Studies of Selected Enzyme Mechanisms

    Introduction

    Pancreatic Ribonuclease

    Chymotrypsin

    Lysozyme

    Creatine Kinase

    References

    8· Regulation of Enzyme Activity

    Introduction

    Experimental Manifestations of Regulatory Enzymes

    Equilibrium Binding Isotherms

    Molecular Models for Allosterism

    Steady-State Kinetics

    Protein Polymerization-Depolymerization

    Hysteretic Enzymes

    Transient Kinetics

    Conclusion

    References

    9. Case Studies of Selected Regulatory Enzymes

    Introduction

    Aspartate Transcarbamoylase

    Phosphofructokinase

    References

    10. Multienzyme Complexes

    Introduction

    General Mechanistic Principles

    Tryptophan Synthase

    Pyruvate and α-Ketoglutarate Dehydrogenase

    Fatty Acid Synthase

    Conclusion

    References

    11. Membrane-Bound Enzymes

    Introduction

    Membrane Structure and Function

    ß-Hydroxybutyrate Dehydrogenase

    (Na+ + K+)-Activated ATPase

    ATP Synthesis

    Adenylate Cyclase

    Conclusion

    References

    Appendix: Practice Problems

    Index

Product details

  • No. of pages: 276
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: January 28, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9780323158176

About the Author

Gorden Hammes

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