Enzyme Catalysis and Regulation - 1st Edition - ISBN: 9780123219602, 9780323158176

Enzyme Catalysis and Regulation

1st Edition

Authors: Gorden Hammes
eBook ISBN: 9780323158176
Imprint: Academic Press
Published Date: 28th January 1982
Page Count: 276
Sales tax will be calculated at check-out Price includes VAT/GST
25% off
25% off
25% off
25% off
25% off
20% off
20% off
25% off
25% off
25% off
25% off
25% off
20% off
20% off
25% off
25% off
25% off
25% off
25% off
20% off
20% off
72.95
54.71
54.71
54.71
54.71
54.71
58.36
58.36
54.95
41.21
41.21
41.21
41.21
41.21
43.96
43.96
43.99
32.99
32.99
32.99
32.99
32.99
35.19
35.19
Unavailable
Price includes VAT/GST
× DRM-Free

Easy - Download and start reading immediately. There’s no activation process to access eBooks; all eBooks are fully searchable, and enabled for copying, pasting, and printing.

Flexible - Read on multiple operating systems and devices. Easily read eBooks on smart phones, computers, or any eBook readers, including Kindle.

Open - Buy once, receive and download all available eBook formats, including PDF, EPUB, and Mobi (for Kindle).

Institutional Access

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Description

Enzyme Catalysis and Regulation is an introduction to enzyme catalysis and regulation and covers topics ranging from protein structure and dynamics to steady-state enzyme kinetics, multienzyme complexes, and membrane-bound enzymes. Case studies of selected enzyme mechanisms are also presented. This book consists of 11 chapters and begins with a brief overview of enzyme structure, followed by a discussion on methods of probing enzyme structure such as X-ray crystallography and optical spectroscopy. Kinetic methods are then described, with emphasis on the general principles of steady-state and transient kinetics. The chemical principles involved in enzyme catalysis are also discussed, and case studies of a few well-documented enzymes are presented. The regulation of enzyme activity is analyzed from a nongenetic viewpoint, with particular reference to binding isotherms and models for allosterism. Two particular enzymes, aspartate transcarbamoylase and phosphofructokinase, are used as examples of well-studied regulatory enzymes. The last two chapters focus on multienzyme complexes and membrane-bound enzymes. This monograph is intended for graduate students, advanced undergraduates, and research workers in molecular biology and biochemistry.

Table of Contents


Preface

1. Protein Structure and Dynamics

References

General References on Proteins and Enzymes

2. Probes of Enzyme Structure

Introduction

Amino Acid Sequencing

X-Ray Crystallography

Chemical Modification

Optical Spectroscopy

Nuclear Magnetic Resonance

Spin Labels

References

3. Steady-State Enzyme Kinetics

Introduction

Single Substrate-Single Product

King-Altman Method

Inhibition of Enzyme Catalysis

Multiple Substrates

Isotope Exchange at Equilibrium

pH Dependence of Enzyme Catalysis

Temperature Dependence of Enzyme Catalysis

Conclusion

References

4. Transient Kinetic Methods for Studying Enzymes

Introduction

Rapid Mixing Methods

Chemical Relaxation Methods

General Solutions of Kinetic Equations

Rate Equations near Equilibrium

References

5. Some Chemical Aspects of Enzyme Catalysis

Catalysis

Acid-Base Catalysis

Nucleophilic Catalysis

Electrophilic Catalysis

Stereochemistry

Kinetic Isotope Effects

References

6. Elementary Steps in Enzyme Catalysis

Introduction

Binding of Substrates

Conformational Changes

Acid-Base Catalysis

Covalent Intermediates

Advantages of Enzymes

References

7· Case Studies of Selected Enzyme Mechanisms

Introduction

Pancreatic Ribonuclease

Chymotrypsin

Lysozyme

Creatine Kinase

References

8· Regulation of Enzyme Activity

Introduction

Experimental Manifestations of Regulatory Enzymes

Equilibrium Binding Isotherms

Molecular Models for Allosterism

Steady-State Kinetics

Protein Polymerization-Depolymerization

Hysteretic Enzymes

Transient Kinetics

Conclusion

References

9. Case Studies of Selected Regulatory Enzymes

Introduction

Aspartate Transcarbamoylase

Phosphofructokinase

References

10. Multienzyme Complexes

Introduction

General Mechanistic Principles

Tryptophan Synthase

Pyruvate and α-Ketoglutarate Dehydrogenase

Fatty Acid Synthase

Conclusion

References

11. Membrane-Bound Enzymes

Introduction

Membrane Structure and Function

ß-Hydroxybutyrate Dehydrogenase

(Na+ + K+)-Activated ATPase

ATP Synthesis

Adenylate Cyclase

Conclusion

References

Appendix: Practice Problems

Index

Details

No. of pages:
276
Language:
English
Copyright:
© Academic Press 1982
Published:
Imprint:
Academic Press
eBook ISBN:
9780323158176

About the Author

Gorden Hammes

Ratings and Reviews