Enzyme Activity in Single Cells

Enzyme Activity in Single Cells

1st Edition - October 29, 2019

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  • Editors: Nancy Allbritton, Michelle Kovarik
  • Hardcover ISBN: 9780128170908
  • eBook ISBN: 9780128170915

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Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on Enzyme Activity in Single Cells


Cell biologist focused on measuring cellular physiology at the single-cell level as well as scientists and engineers seeking to develop highly sensitive technologies for cutting edge cell-based assays

Table of Contents

  • 1. Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome
    Songon An, Miji Jeon, Erin L. Kennedy and Minjoung Kyoung
    2. Synthetic probe development for measuring single or few-cell activity and efflux
    Alison Lui, Jeffrey Wang, Linda Chio and Markita P. Landry
    3. Enzymatic activity in single cells
    Josephine Geertsen Keller, Magnus Stougaard and Birgitta R. Knudsen
    4. Single-cell assays using integrated continuous-flow microfluidics
    Ee Xien Ng, Myat Noe Hsu, Guoyun Sun and Chia-Hung Chen
    5. Single-cell activity screening in microfluidic droplets
    Stefanie Neun, Tomasz S. Kaminski and Florian Hollfelder
    6. The microfluidic capture of single breast cancer cells for multi-drug resistance assays
    Karan Parekh, Hamide Sharifi, Avid Khamenehfar, Timothy V. Beischlag, Robert T.M. Payer and Paul C.H. Li
    7. Single-cell proteolytic activity measurement using microfluidics for rare cell populations
    Yu-Chih Chen and Euisik Yoon
    8. Microfluidic technology for investigation of protein function in single adherent cells
    Aldo Jesorka, Inga Põldsalu and Irep Gözen
    9. Nanokits for the electrochemical quantification of enzyme activity in single living cells
    Rongrong Pan and Dechen Jiang
    10. Design of an automated capillary electrophoresis platform for single-cell analysis
    David H. Abraham, Matthew M. Anttila, Luke A. Gallion, Brae V. Petersen, Angela Proctor and
    Nancy L. Allbritton
    11. Microelectrophoretic single-cell measurements with microfluidic devices
    Jay Sibbitts, Jalal Sadeghi and Christopher T. Culbertson
    12. Chemical probes for spatially resolved measurement of active enzymes in single cells
    Gang Li and Raymond E. Moellering
    13. Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry
    Camille Lombard-Banek, Sam B. Choi and Peter Nemes
    14. Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells
    Ruichuan Yin, Venkateshkumar Prabhakaran and Julia Laskin

Product details

  • No. of pages: 326
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: October 29, 2019
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128170908
  • eBook ISBN: 9780128170915

About the Serial Volume Editors

Nancy Allbritton

Nancy Allbritton
Nancy L. Allbritton is the Kenan Professor of Chemistry and Biomedical Engineering and Chair of the Joint Department of Biomedical Engineering at the University of North Carolina at Chapel Hill (UNC) and North Carolina State University (NC State). Her research focuses on the development of novel technologies for applications in single-cell analysis, micro-arrays and fluidics, and organ-on-chip. Dr. Allbritton is a Fellow of the American Association for the Advancement of Science, the American Institute for Medical & Biological Engineering, and the National Academy of Inventors. She obtained her B.S. in physics from Louisiana State University, M.D. from Johns Hopkins University, and Ph.D. in Medical Physics/Medical Engineering from the Massachusetts Institute of Technology, with a postdoctoral fellowship at Stanford University.

Affiliations and Expertise

University of North Carolina, USA

Michelle Kovarik

Michelle Kovarik
Michelle Kovarik is a bioanalytical chemist with particular interests in microfluidics, single-cell assays, and cellular responses to stress. She received her undergraduate degree from Saint Louis University and her doctoral degree from Indiana University. After a SPIRE postdoctoral appointment at the University of North Carolina, she began a tenure-track position at Trinity College, an undergraduate liberal arts college in Hartford, CT. In addition to her laboratory research, she also makes professional contributions in the area of chemistry education.

Affiliations and Expertise

Trinity College, Hartford, CT, USA

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