Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on the soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens--bacteria, viruses, and protozoan parasites--used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.
- New edition incorporates new experiments and the latest techniques
- Designed for maximum versatility and ease of use for the student and instructor
- Each experiment is self-contained and includes theoretical, practical, and pedagogical material.
Professional microbiologists working with soil and water; upper-division undergraduate and graduate students studying environmental microbiology.
Basic Protocols Experiment 1 - Dilution and Plating of Bacteria and Growth Curves Experiment 2 - Soil Moisture Content Determination
Examination of Soil Microorganisms via Microscopic and Cultural Assays Experiment 3 - Contact Slide Assay Experiment 4 - Filamentous Fungi Experiment 5 - Bacteria and Actinomycetes Experiment 6 - Algae: Enumeration by MPN
Microbial Transformations and Response to Contaminants Experiment 7 - Oxidation of Sulfur in Soil Experiment 8 - Dehydrogenase Activity of Soils Experiment 9 - Nitrification and Denitrification Experiment 10 - Enrichment and Isolation of Bacteria that Degrade 2, 4-dichlorophenoxyacetic acid Experiment 11 - Adaptation of Soil Bacteria to Metals Experiment 12 - Biodegradation of Phenol Compounds Experiment 13 - Assimilable Organic Matter Experiment 14 - Biochemical Oxygen Demand
Water Microbiology Experiment 15 - Membrane Filter Technique Experiment 16 - Bacteriological Examination of Water: the Coliform MPN Test Experiment 17 - Detection of Bacteriophages Experiment 18 - Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces Experiment 19 - Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms
Advanced Topics Experiment 20 - Detection of Enteric Viruses in Water Experiment 21 - Detection of Waterborne Parasites Experiment 22 - Kinetics of Disinfection Experiment 23 - Aerobiology: Sampling of Airborne Microorganisms Experiment 24 - Detection and Identification of Bacteria via PCR and Subsequent BL
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"I would strongly recommend this laboratory manyal for any environmental microbiology course. It comes as a perfect complementary laboratory manual to the textbook "Environmental Microbiology" by R.M. Maier, I.L. Pepper and C.P. Gerber." - Dr. Hanan El-Mayas, Georgia State University "Environmental Microbiology: A Laboratory Manual provides a very good introduction to a variety of approaches used to assess microorganisms in the environment." - Deborah T. Newby, Idaho National Laboratory "The book has been written by two outstanding microbiologists and addresses critical methodological techniques for microbial analyses. The manual is designed for upper division and graduate-level laboratories in environmental microbiology. However, every laboratory dealing with microbes should have this excellent manual. In particular, I highly recommend it for laboratories that assess microbial risks and advantages. These include wastewater and water-treatment plants, soil-carbon-sequestration centers, and forensics and homeland-security laboratories. The bio-threat agents covered in the manual include bacteria (anthrax), viruses, and protozoan parasites (Cryptosporidium and Giardia). The new methods of polymerase chain reaction (PCR) and sequence analysis are given. Sample problems allow the self-study student to follow correct procedures. The glossary is a valuable appendix. I thank the authors for making this information available to the many scientists and technicians who will benefit from it." - M.B. Kirkham, Kansas State University and author of Principles of Soil and Plant Water Relations "[The manual] familiarizes the students with basic procedures and hands on experiments that are used as standard procedures by environmental agencies such as EPA for assessing waste treatment quality, water and food quality....The experimental pro