Environmental Microbiology

Environmental Microbiology

A Laboratory Manual

2nd Edition - December 13, 2004

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  • Authors: Ian Pepper, Charles Gerba, Jeffrey Brendecke
  • eBook ISBN: 9780080470511

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Description

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on the soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens--bacteria, viruses, and protozoan parasites--used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Key Features

  • Incorporates new experiments and the latest techniques
  • Designed for maximum versatility and ease of use for the student and instructor
  • Each experiment is self-contained and includes theoretical, practical, and pedagogical material

Readership

Professional microbiologists working with soil and water; upper-division undergraduate and graduate students studying environmental microbiology.

Table of Contents

  • Preface
    Basic Protocols
    Experiment 1 - Dilution and Plating of Bacteria and Growth Curves
    Experiment 2 - Soil Moisture Content Determination
    Examination of Soil Microorganisms via Microscopic and Cultural Assays
    Experiment 3 - Contact Slide Assay
    Experiment 4 - Filamentous Fungi
    Experiment 5 - Bacteria and Actinomycetes
    Experiment 6 - Algae: Enumeration by MPN
    Microbial Transformations and Response to Contaminants
    Experiment 7 - Oxidation of Sulfur in Soil
    Experiment 8 - Dehydrogenase Activity of Soils
    Experiment 9 - Nitrification and Denitrification
    Experiment 10 - Enrichment and Isolation of Bacteria that Degrade 2, 4-dichlorophenoxyacetic acid
    Experiment 11 - Adaptation of Soil Bacteria to Metals
    Experiment 12 - Biodegradation of Phenol Compounds
    Experiment 13 - Assimilable Organic Matter
    Experiment 14 - Biochemical Oxygen Demand
    Water Microbiology
    Experiment 15 - Membrane Filter Technique
    Experiment 16 - Bacteriological Examination of Water: the Coliform MPN Test
    Experiment 17 - Detection of Bacteriophages
    Experiment 18 - Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces
    Experiment 19 - Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms
    Advanced Topics
    Experiment 20 - Detection of Enteric Viruses in Water
    Experiment 21 - Detection of Waterborne Parasites
    Experiment 22 - Kinetics of Disinfection
    Experiment 23 - Aerobiology: Sampling of Airborne Microorganisms
    Experiment 24 - Detection and Identification of Bacteria via PCR and Subsequent BLAST Analysis of Amplified Sequences
    Appendix 1: Preparation of Media and Stains for Each Experiment
    Appendix 2: Glossary

Product details

  • No. of pages: 232
  • Language: English
  • Copyright: © Academic Press 2011
  • Published: December 13, 2004
  • Imprint: Academic Press
  • eBook ISBN: 9780080470511

About the Authors

Ian Pepper

Ian Pepper
Dr. Ian Pepper is currently a Professor at the University of Arizona. He is also Director of the University of Arizona, Environmental Research Laboratory (ERL) and the NSF Water and Environmental Technology (WET) Center. Dr. Pepper is an environmental microbiologist specializing in the molecular ecology of the environment. His research has focused on the fate and transport of pathogens in air, water, soils and wastes. His expertise has been recognized by membership on six National Academy of Science Committees and former memberships on an EPA FIFRA Science and Advisory Panel. Dr. Pepper is a Fellow of the American Association for the Advancement of Science, American Academy of Microbiology, the Soil Science Society of America, and the American Society of Agronomy. He is also a Board Certified Environmental Scientist within the American Academy of Environmental Engineers and Scientists. He is the author or co-author of six textbooks; 40 book chapters; and over 180 peer-review journal articles.

Affiliations and Expertise

Professor at the University of Arizona; Director of the University of Arizona, Environmental Research Laboratory (ERL) and the NSF Water and Environmental Technology (WET) Center; environmental microbiologist specializing in the molecular ecology of the environment.

Charles Gerba

Charles Gerba
Dr. Charles P. Gerba is a Professor at the University of Arizona. He conducts research the transmission of pathogens through the environment. His recent research encompasses the transmission of pathogens by water, food and fomites; fate of pathogens in land applied wastes; development of new disinfectants; domestic microbiology and microbial risk assessment. He has been an author on more than 500 articles including several books in environmental microbiology and pollution science. He is a fellow of the American Academy of Microbiology and the American Association for the Advancement of Science. In 1998 he received the A. P. Black Award from the American Water Works Association for outstanding contributions to water science and in 1996 he received the McKee medal from the Water Environment Federation for outstanding contributions to groundwater protection. He received the 1999 Award of Excellence in Environmental Health from National Association of County and City Health Officials.

Affiliations and Expertise

Professor at the University of Arizona.

Jeffrey Brendecke

Affiliations and Expertise

Anagennesis Custom Graphic Solutions, Tucson, Arizona, U.S.A.

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