Volume 397. Environmental Microbiology

I. Cultivation 1. Anoxic media design, preparation, and considerations; 2. Enrichment and isolation of anaerobic hydrocarbon degraders; 3. Anammox organisms: enrichment, cultivation, and environmental analysis; 4. Enrichment and isolation of metal and humic acid reducing microbes; 5. Enrichment, cultivation, and detection of reductively dechlorinating bacteria; 6. Enrichment and isolation of iron-oxidizing bacteria at neutral pH; 7. High throughput cultivation of microorganisms using microcapsules II. Physiological Ecology 8. Chemotaxis and behavioral physiology of not-yet-cultivated microbes; 9. Analysis of microbial communities with electrochemical microsensors and microscale biosensors; 10. Optical microsensors for analysis of microbial communities; 11. In situ measurements of metabolite fluxes: microinjection of radiotracers into insect guts and other small compartments; 12. Assaying for the hydroxypropionate pathway of carbon fixation; 13. Analysis of trace hydrogen metabolism ; 14. Advances in microscopy: microautoradiography of single cells; 15. Atomic Force Microscopy of Bacterial Communities III. Nucleic Acid Techniques 16. Nucleic acid recovery from complex environmental samples; 17. The application of rarefaction techniques to molecular inventories of microbial diversity; 18. Culture-independent microbial community analysis with terminal restriction fragment length polymorphism; 19. Quantitative community analysis – capillary electrophoresis techniques; 20. In situ functional gene analysis: RING-FISH; 21. Simultaneous fluorescence in situ hybridization of mRNA and rRNA for the detection of gene expression in environmental microbes; 22. Community-level analysis of phototrophy: psbA gene diversity; 23. Quantitative analysis of nifH genes and transcripts from aquatic environments; 24. Community l