Engineered Nanopores for Bioanalytical Applications

Engineered Nanopores for Bioanalytical Applications

1st Edition - March 4, 2013

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  • Authors: Joshua B. Edel, Tim Albrecht
  • Hardcover ISBN: 9781437734737
  • eBook ISBN: 9781437734744

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Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding.This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices.

Key Features

  • Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials
  • Introduces the techniques of manufacturing solid state materials with functional nanopores
  • Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics


Biomedical Engineering; Applied research scientists / engineers involved in the applied R&D areas of bio micro-nanofluidics, genetic engineering, Micro/nanofabrication, BioNEMS, bionanomaterials; Pharmaceuticals Engineers and Biotechnology Industry Scientists working on drug discovery, drug delivery methods, safety and drug screening; Biomedical laboratory researchers working on new methods for Tissue Engineering, Stem Cells, and Regenerative Medicine technologies; BioNEMS , biosensors, and micro- nanofluidics industries

Table of Contents

  • List of Contributors
    Engineered nanopores for bioanalytical applications

    1. Ion Transport in Nanopores
    1.1 Introduction
    1.2 Brownian motion
    1.3 Net transport of ions: the Nernst–Planck equation and its derivation
    1.4 The conductance of a pore with uncharged walls
    1.5 The effect of surface charge
    1.6 Particle translocation through nanopores—the model of deBlois and Bean

    2. DNA Translocation
    2.1 Introduction
    2.2 Physics of a polyelectrolyte inside a nanopore
    2.3 Electroosmotic flow inside a cylindrically nanopore
    2.4 DNA inside a nanopore
    2.5 Capture rate and probability of successful translocation
    2.6 Stalling DNA in a nanopore
    2.7 Stalling DNA in nanocapillaries

    3. Instrumentation for Low-Noise High-Bandwidth Nanopore Recording
    3.1 Introduction
    3.2 Components of a nanopore setup and their integration
    3.3 Low-current measurement techniques
    3.4 Bandwidth and background noise
    3.5 Noise filtering, sampling, and resolution
    3.6 Outlook: pushing the detection limit

    4. Biological Pores on Lipid Bilayers
    4.1 Introduction
    4.2 Formation: overview and experimental protocols
    4.3 Pore characterization: overview and experimental protocols
    4.4 Bacterial pore-forming toxins
    4.5 Bacterial porins
    4.6 Pore-forming peptides
    4.7 Conclusion

    5. Solid-State Nanopore Fabrication
    5.1 Introduction
    5.2 Overview of materials
    5.3 Fabrication methods
    5.4 Control of pore size
    5.5 Surface modification

    6. Case Studies Using Solid-State Pores
    6.1 Introduction
    6.2 DNA and RNA translocations
    6.3 DNA unzipping
    6.4 Optical detection
    6.5 DNA sequencing
    6.6 Protein analysis with solid-state nanopores
    6.7 Conclusion

Product details

  • No. of pages: 192
  • Language: English
  • Copyright: © William Andrew 2013
  • Published: March 4, 2013
  • Imprint: William Andrew
  • Hardcover ISBN: 9781437734737
  • eBook ISBN: 9781437734744

About the Authors

Joshua B. Edel

Affiliations and Expertise

Imperial College, London, UK

Tim Albrecht

Affiliations and Expertise

Imperial College London

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