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Energetics of Biological Macromolecules - 1st Edition - ISBN: 9780121821609, 9780080883724

Energetics of Biological Macromolecules, Volume 259

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Michael Johnson Gary Ackers
eBook ISBN: 9780080883724
Imprint: Academic Press
Published Date: 25th September 1995
Page Count: 761


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Table of Contents

J.M. Holt and G.K. Ackers, Pathway of Allosteric Control as Revealed by Intermediate States of Hemoglobin. Y. Huang and D.W. Bolen, Probes of Energy Transduction in Enzyme Catalysis. V.A. Parsegian, R.P. Rand, and D.C. Rau, Macromolecules and Water: Probing with Osmotic Stress. I. Wong and T.M. Lohman, Linkage of Protein Assembly to Protein-DNA Binding. E. Di Cera, Q.D. Dang, Y. Ayala, and A. Vindigni, Linkage at Steady State: Allosteric Transitions of Thrombin. E. Freire, Thermal Denaturation Methods in Study of Protein Folding. L. Chen, R.L. Biltonen, and M.L. Johnson, Kinetics of Lipid Membrane Phase Transitions: A Volume Perturbation Calorimeter Study. M.L. Doyle, G. Louie, P. Dal Monte, and T. Sokoloski, Tight Binding Affinities Determined from the Thermodynamic Linkage to Protons by Titration Calorimetry. H.F. Fisher and N. Singh, Calorimetric Methods for Interpreting Protein-Ligand Interactions. K.J. Breslauer, Extracting Thermodynamic Data from Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions. M.J. Serra and D.H. Turner, Predicting Thermodynamic Properties of RNA. K.B. Hall and J.K. Kranz, Thermodynamics and Mutations in RNA-Protein Interactions. D.E. Draper and T.C. Gluick, Melting Studies of RNA Unfolding and RNA-Ligand Interactions. L. Jen-Jacobson, Structural-Perturbation Approaches to Thermodynamics of Site-Specific Protein-DNAInteractions. Y. Bai, J.J. Englander, L. Mayne, J.S. Milne, and S.W. Englander, Thermodynamic Parameters from Hydrogen Exchange Measurements. C.A. Royer, Application of Pressure to Biochemical Equilibria: The Other Thermodynamic Variable. L.M. Rellick and W.J. Becktel, Molecular Volume. C.R. Robinson and S.G. Sligar, Hydrostatic and Osmotic Pressure as Tools to Study Macromolecular Recognition. T.M. Laue, Sedimentation Equilibrium as Thermodynamic Tool. J. Yang and J. Carey, Footprint Phenotypes: Structural Models of DNA-Binding Proteins from Chemical Modification Analysis of DNA. M. Perrella and I. Denisov, Low-Temperature Electrophoresis Methods. M.R. Eftink, Use of Multiple Spectroscopic Methods to Monitor Equilibrium Unfolding of Proteins. B. Garcia-Moreno, Probing Structural and Physical Basis of Protein Energetics Linked to Protons and Salt. C.N. Pace, Evaluating Contribution of Hydrogen Bonding and Hydrophobic Bonding to Protein Folding. B. Lee, Analyzing Solvent Reorganization and Hydrophobicity. T.P. Creamer and G.D. Rose, Simple Force Field for Study of Peptide and Protein Conformational Properties. M. Martinez-Carrion, A. Artigues, A. Berezov, M.L. Bianconi, A.M. Reyes, and A. Iriarte, Probes for Analysis of Stability of Different Variants of Aspartate Aminotransferase. N.M. Allewell and V.J. LiCata, Thermodynamic Approaches to Understanding Aspartate Transcarbamylase. R. Lumry, On the Interpretation of Data from Isothermal Processes. Author Index. Subject Index.


The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Thermodynamics as a tool for understanding molecular logic

  • Thermal denaturation methods in the study of protein folding
  • Predicting thermodynamic properties of RNA
  • Sedimentation equilibrium as a thermodynamic tool
  • Molecular volume
  • Thermodynamic parameters from hydrogen exchange measurements


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


No. of pages:
© Academic Press 1995
25th September 1995
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Michael Johnson

Affiliations and Expertise

University of Virginia Health Sciences Center, Charlottesville, USA

Gary Ackers

Affiliations and Expertise

Washington University School of Medicine, St. Louis, Mo, USA