Description

This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed.

Key Features

*Methods for Evaluating Cooperativity in a Dimeric Hemoglobin *Multiple-Binding of Ligands to a Linear Biopolymer *Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions *Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis

Readership

Biochemists, biophysicists, bioengineers, molecular biologists, structural biologists

Table of Contents

Analyzing Intermediate State Cooperativity in Hemoglobin; Nuclear Magnetic Resonance Spectroscopy in the Study of Hemoglobin Cooperativity; Evaluating Cooperativity in Dimeric Hemoglobins; Measuing Assembly and Binding in Human Embryonic Hemoglobins; Small-Angle Scattering Techniques for Analyzing; Conformational Transitions in Hemocyanins; Multivalent Protein-Carbohydrate Interactions: Isothermal Titration Microcalorimetry Studies; Calorimetric Analysis of Mutagenic Effects on Protein-Ligand Interactions; Multiple Binding of Ligands to a Linear Biopolymer; Probing Site-Specific Energetics in Proteins and Nucleic Acids by Hydrogen Exchange and Nuclear Magnetic Resonance Spectroscopy; Fluorescence Quenching Methods to Study Protein-Nucleic Acid interactions; Thermodynamics, Protein Modification, and Molecular Dynamics in Characterizing Lactose Repressor Protein: Strategies for Complex Analyses of Protein Structure-Function; Linked Equilibria in Biotin Repressor Function: Thermodynamics, Structural, and Kinetic Analysis; Distance Parameters Derived from Time-Resolved Förster Resonance Energy Transfer Measurements and Their Use in Structural Interpretations of Thermodynamic Quantities Associated with Protein-DNA Interactions

Details

No. of pages:
281
Language:
English
Copyright:
© 2004
Published:
Imprint:
Academic Press
eBook ISBN:
9780080497174
Print ISBN:
9780121827830

About the editors

Jo Holt

Affiliations and Expertise

Washington University Medical Center, St. Louis, MO, USA

Michael Johnson

Affiliations and Expertise

University of Virginia Health Sciences Center, Charlottesville, USA

Gary Ackers

Affiliations and Expertise

Washington University School of Medicine, St. Louis, Mo, USA

Reviews

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." CHEMISTRY IN INDUSTRY