The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Thermodynamics as a tool for understanding molecular logic * Thermal denaturation methods in the study of protein folding * Predicting thermodynamic properties of RNA * Sedimentation equilibrium as a thermodynamic tool * Molecular volume * Thermodynamic parameters from hydrogen exchange measurements


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

J.M. Holt and G.K. Ackers, Pathway of Allosteric Control as Revealed by Intermediate States of Hemoglobin. Y. Huang and D.W. Bolen, Probes of Energy Transduction in Enzyme Catalysis. V.A. Parsegian, R.P. Rand, and D.C. Rau, Macromolecules and Water: Probing with Osmotic Stress. I. Wong and T.M. Lohman, Linkage of Protein Assembly to Protein-DNA Binding. E. Di Cera, Q.D. Dang, Y. Ayala, and A. Vindigni, Linkage at Steady State: Allosteric Transitions of Thrombin. E. Freire, Thermal Denaturation Methods in Study of Protein Folding. L. Chen, R.L. Biltonen, and M.L. Johnson, Kinetics of Lipid Membrane Phase Transitions: A Volume Perturbation Calorimeter Study. M.L. Doyle, G. Louie, P. Dal Monte, and T. Sokoloski, Tight Binding Affinities Determined from the Thermodynamic Linkage to Protons by Titration Calorimetry. H.F. Fisher and N. Singh, Calorimetric Methods for Interpreting Protein-Ligand Interactions. K.J. Breslauer, Extracting Thermodynamic Data from Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions. M.J. Serra and D.H. Turner, Predicting Thermodynamic Properties of RNA. K.B. Hall and J.K. Kranz, Thermodynamics and Mutations in RNA-Protein Interactions. D.E. Draper and T.C. Gluick, Melting Studies of RNA Unfolding and RNA-Ligand Interactions. L. Jen-Jacobson, Structural-Perturbation Approaches to Thermodynamics of Site-Specific Protein-DNAInteractions. Y. Bai, J.J. Englander, L. Mayne, J.S. Milne, and S.W. Englander, Thermodynamic Parameters from Hydrogen Exchange Measurements. C.A. Royer, Application of Pressure to Biochemical Equilibria: The Other Thermodynamic Variable. L.M. Rellick and W


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© 1995
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY