Embryonic Stem Cells
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This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.
Key Features
* Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
* Presents the latest most innovative technologies
* Addresses therapeutic relevance including FDA compliance and tissue engineering
* Presents the latest most innovative technologies
* Addresses therapeutic relevance including FDA compliance and tissue engineering
Readership
Biochemists, cell and molecular biologists, and bioengineers working with stem cells.
Table of Contents
- SECTION I. DERIVATION AND MAINTENANCE OF EMBRYONIC STEM CELLS
Murine embryonic stem cells.
Bovine embryonic Stem Cells.
Avian embryonic stem cells.
Zebrafish embryonic stem cells.
Human embryonic stem cells.
Embryonic stem cells from morula.
Embryonic stem cells from single-blastomeres.
Embryonic stem cells from parthenotes.
Embryonic stem cells using nuclear transfer.
SECTION II. DIFFERENTIATION OF EMBRYONIC STEM CELLS
Neurons and glia.
Retinal pigment epithelium.
Mesenchimal cells.
Hematopoietic cells.
Hematopoietic cells from primate ES cells.
Vascular cells.
Cardiomyocytes.
Oocytes.
Male germ cells.
Insulin-producing cells.
Pulmonary epithelium.
Product details
- No. of pages: 432
- Language: English
- Copyright: © Academic Press 2006
- Published: November 20, 2006
- Imprint: Academic Press
- eBook ISBN: 9780080471129
About the Serial Volume Editors
Irina Klimanskaya
Affiliations and Expertise
Advance Cell Technology, Worcester, MA, USA
Robert Lanza
Robert Lanza is an American scientist and author whose research spans the range of natural science, from biology to theoretical physics. TIME magazine recognized him as one of the “100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 “World Thinkers.” He has hundreds of scientific publications and over 30 books, including definitive references in the fields of stem cells, tissue engineering, and regenerative medicine. He is a former Fulbright Scholar and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman (known for his work on the biological basis of consciousness) and Rodney Porter. He also worked closely (and co-authored papers in Science on self-awareness and symbolic communication) with noted Harvard psychologist BF Skinner. Dr. Lanza was part of the team that cloned the world’s first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.
Currently Dr. Lanza is the Head of Astellas Global Regenerative Medicine and Chief Scientific Officer of the Astellas Institute for Regenerative Medicine. He is also an Adjunct Professor at Wake Forest University School of Medicine and has authored over 200+ articles and over a dozen books.
Affiliations and Expertise
Astellas Institute for Regenerative Medicine, Westborough, MA, USA