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SECTION I. DERIVATION AND MAINTENANCE OF EMBRYONIC STEM CELLS
Murine embryonic stem cells.
Bovine embryonic Stem Cells. Avian embryonic stem cells. Zebrafish embryonic stem cells. Human embryonic stem cells.
Embryonic stem cells from morula. Embryonic stem cells from single-blastomeres. Embryonic stem cells from parthenotes. Embryonic stem cells using nuclear transfer.
SECTION II. DIFFERENTIATION OF EMBRYONIC STEM CELLS
Neurons and glia.
Retinal pigment epithelium.
Hematopoietic cells. Hematopoietic cells from primate ES cells. Vascular cells. Cardiomyocytes. Oocytes. Male germ cells.
Insulin-producing cells. Pulmonary epithelium.
This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.
- Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
- Presents the latest most innovative technologies
- Addresses therapeutic relevance including FDA compliance and tissue engineering
Biochemists, cell and molecular biologists, and bioengineers working with stem cells.
- No. of pages:
- © Academic Press 2006
- 22nd November 2006
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Advance Cell Technology, Worcester, MA, USA
Robert Lanza, M.D. is currently Head of Astellas Global Regenerative Medicine, Chief Scientific Officer of AIRM and an adjunct professor at the Wake Forest Institute for Regenerative Medicine. Time magazine recognized him as one of the “100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 “World Thinkers.” His research focuses on stem cells and their potential to provide therapies for some of the world's most deadly and debilitating conditions. He has hundreds of scientific publications and over 30 books, including definitive references in the fields of tissue engineering and regenerative medicine. He is a former Fulbright Scholar, and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman and Rodney Porter. He also worked closely (and co-authored a series of papers) with psychologist BF Skinner and heart transplant-pioneer Christiaan Barnard. Dr. Lanza received his undergraduate and medical degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. Lanza was part of the team that cloned the world’s first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.
Astellas Institute for Regenerative Medicine, MA, USA