Electrophoresis in Stabilizing Media

Paper Chromatography and Electrophoresis

1st Edition - January 1, 1967
Author: John Whitaker
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 2 0 9 5 - 6

Paper Chromatography and Electrophoresis, Volume I: Electrophoresis in Stabilizing Media covers the general features of electrophoresis in stabilizing media. The book includes a… Read more

Purchase options

LIMITED OFFER

Save 50% on book bundles

Immediately download your ebook while waiting for your print delivery. No promo code is needed.

Institutional subscription on ScienceDirect

Request a sales quote

Paper Chromatography and Electrophoresis, Volume I: Electrophoresis in Stabilizing Media covers the general features of electrophoresis in stabilizing media. The book includes a consideration of the factors which determine the rate of movement of the compounds in an electrical field, the factors which must be controlled in order to obtain successful results, as well as the general arrangement and types of equipment used. The text also provides a description of methods for the separation of specific classes of compounds (amines, amino acids, peptides, proteins, nucleic acids, derivatives, and related compounds, carbohydrates, and organic acids and derivatives) normally encountered by chemists. Inorganic chemists, organic chemists, clinical chemists, and biochemists will find the book invaluable.

DedicationPrefaceChapter 1 General Considerations I. Terminology II. Theoretical Considerations III. Types of Stabilizing Media IV. Apparatus V. Two-Dimensional Separations VI. Crossing Electrophoresis VII. Continuous Electrophoresis VIII. Column Electrophoresis IX. Application of Sample X. Reference Compounds and Standards XI. Application of Electrical Potential XII. Detection of Components XIII. Quantitation XIV. Interpretation of Results ReferencesChapter 2 Amines, Amino Acids, and Peptides I. General Considerations II. Amines III. Amino Acids IV. Dinitrophenyl (DNP) Amino Acids and Related Compounds V. Electrophoretic Separation of Peptides ReferencesChapter 3 Proteins I. General Considerations II. Preparation of Sample III. Supporting Materials and Techniques IV. Other Methods of Detecting and Identifying Protein Zones ReferencesChapter 4 Nucleic Acids, Derivatives, and Related Compounds I. General Considerations II. Purine and Pyrimidine Bases and Nucleosides III. Nucleotides IV. Separation of the Isomeric Nucleoside Monophosphates V. Oligonucleotide Mapping Procedures VI. Thin-Layer Electrophoresis VII. Ion Exchange Electrophoresis VIII. Ultramicroelectrophoretic Separation of Nucleotides IX. Separation of Nucleic Acids ReferencesChapter 5 Carbohydrates I. General II. Mono- and Oligosaccharides III. Neutral and Acidic Polysaccharides IV. Sugar Phosphates V. Polyhydric Alcohols VI. Amino Sugars VII. Sugar Acids VIII. Methyl Glycosides ReferencesChapter 6 Organic Acids and Derivatives I. General II. The Saturated Monocarboxylic Acids III. Aliphatic Poly functional Carboxylic Acids and Aromatic Acids IV. Organic Phosphates V. Phenols and Derivatives ReferencesChapter 7 Electrophoretic Separation of Other Organic Compounds I. Aldehydes and Ketones II. Alkaloids III. Antibiotics IV. Choline Esters and Phospholipid Derivatives V. Coenzymes and Vitamins VI. Water-Insoluble Dyes VII. Water-Soluble Dyes VIII. Natural Pigments IX. Steroids ReferencesChapter 8 Inorganic Ions I. General II. Separation of the More Common Cations and Anions III. The Rare Earths IV. Column Electrophoretic Separation of Inorganic Ions V. Ion-Focusing Electrophoresis ReferencesAuthor IndexSubject Index