Description

The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems.

Key Features

Covers the preparation and analysis of model systems for biological electron microscopy. Includes the most popular systems but also organisms that are less frequently used in cell biology. This issue presents the currently most important methods for the preparation of biological specimens. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems.

Readership

Researches, Scientist and students in Cell Biology

Table of Contents

Preface/Acknowledgements

Thomas Müller-Reichert

1. Electron microscopy of viruses

Michael Laue

2. Bacterial TEM: New insights from cryo-microscopy

Martin Pilhofer, Mark S. Ladinsky, Alasdair W. McDowall, and Grant Jensen*

3. Analysis of the ultrastructure of archaea by electron microscopy

Reinhard Rachel* , Carolin Meyer, Andreas Klingl, Sonja Gürster, Nadine Wasserburger, Ulf Küper, Annett Bellack, Simone Schopf, Reinhard Wirth, Harald Huber, and Gerhard Wanner

4. Chlamydomonas: Cryopreparation methods for the 3-D analysis of cellular organelles

Eileen T. O’Toole

5. Ultrastructure of the asexual blood stages of Plasmodium falciparum

Eric Hanssen* , Kenneth N Goldie, and Leann Tilley

6. Electron tomography and immunolabeling of Tetrahymena thermophila basal bodies

Thomas H. Giddings* Jr., Janet B. Meehl, Chad G. Pearson, and Mark Winey

7. Electron microscopy of Paramecium

Klaus Hausmann* and Richard D. Allen

8. Ultrastructural investigation methods for Trypanosoma brucei

Johanna L. Höög* , Eva Gluenz, Sue Vaughan, and Keith Gull

9. Dictyostelium discoideum: A model system for ultrastructural analyses of cell motility and development

Michael P. Koonce* and Ralpf Gräf

10. Towards sub-second correlative live-cell light and electron microscopy of Saccharomyces cerevisiae

Christopher Buser

11. Fission yeast: A cellular model well suited for electron microscopic investigations

Hélio D. Roque and Claude Antony*

12. High-pressure freezing and electron microscopy of Arabidopsis

Byung-Ho Kang

13. Standard and cryo-preparation of Hydra for transmission electron microscopy

Thomas

Details

No. of pages:
744
Language:
English
Copyright:
© 2010
Published:
Imprint:
Academic Press
eBook ISBN:
9780123810083
Print ISBN:
9780123810076
Print ISBN:
9780323163972

About the serial-volume-editor

Thomas Mueller-Reichert

Dr. Thomas Müller-Reichert is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in meiosis, mitosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in the early embryo of the nematode Caenorhabditis elegans and in tissue culture cells. He got his PhD degree from the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards to the EMBL in Heidelberg (Germany) for a post-doc with Dr. Tony Hyman. He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA) and set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is head of the Core Facility Cellular Imaging (CFCI) of the Medical Faculty Carl Gustav Carus of the TU Dresden (Germany). Together with Dr. Paul Verkade he has developed a rapid transfer system for high-pressure freezing used for Correlative Light and Electron Microcopy. He has organized a number microscopy conferences and taught in several (CL)EM courses. He edited an MCB volume on the Electron Microscopy of Model Systems.

Affiliations and Expertise

Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, Dresden, Germany