Echinoderms - 1st Edition - ISBN: 9780128159545

Echinoderms, Volume 150

1st Edition

Serial Volume Editors: Kathleen Foltz Amro Hamdoun
Hardcover ISBN: 9780128159545
Imprint: Academic Press
Published Date: 1st February 2019
Page Count: 362
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Table of Contents

Section 1 - Procurement and Culturing of Established and Emerging Echinoderm Models
1. Procuring animals and culturing of eggs and embryos
Nikki L. Adams, Kathy R. Foltz, Andreas Heyland and Linda Rice
2. Cryopreservation of sea urchin gametes
Serean Adams and Estefania Paredes
3. Culturing of Amphiura filiformis as a model for gene expression studies
Paola Oliveri
4. Temnopleurus as an emerging echinoderm model
Shunsuke Yaguchi
5. Culturing of sand dollars, cidaroids and heart urchins
Takuya Minokawa, Taku Hibino and Atsuko Yamazaki
6. Culturing and genomics of Lytechinus pictus
Deirdre Lyons
7. Culturing echinoderm larvae through metamorphosis
Jason Hodin

Section 2 - Experimental embryology approaches
8. Microinjection methods
Junko Yaguchi
9. Injection of exogenous messages and protein overexpression
George von Dassow
10. Blastomere transplantation
D R. McClay
11. Cell isolation
Gary M. Wessel
12. Visualizing embryonic polarity
Athula Wikramanayake
13. Methods for the isolation, labeling and live-imaging and analysis of echinoderm primordial germ cells
Joseph Paul Campanale and Amro Hamdoun
14. Methods for studying skeletogenesis
Brian Livingston

Section 3 - Approaches for assessing environmental influences on adults and embryos
15. Larval immune cell approaches
Jon Rast
16. Methods for collection, handling, and analysis of sea urchin coelomocytes
L. Courtney Smith
17. Methods and best practices for assessing the effects of pH on fertilization and development of sea urchins
Sam DuPont and Meike Stump
18. Methods and best practices for toxicology studies in echinoderm embryos and larvae
Gary N. Cherr

Section 4 - Sea urchins in the classroom
19. Sea urchins in the classroom
David Epel and Victor Vacquier
20. A laboratory on xenobiotic transporter activity
Lauren E. Shipp
21. Sea urchin labs.
Laura Romano, Robert Morris and Christine Byrum
22. Analyzing gene expression in sea star embryos
David Carroll


Description

Echinoderms, Volume 150 in the Methods in Cell Biology series, highlights new advances in the field, with this update presenting interesting chapters on procuring animals and culturing of eggs and embryos, cryopreservation of sea urchin gametes, emerging echinoderm models, culturing of sand dollars, cidaroids and heart urchins, culturing echinoderm larvae through metamorphosis, microinjection methods, injection of exogenous messages and protein overexpression, blastomere transplantation, visualization of embryonic polarity, larval immune cell approaches, methods for analysis of sea urchin primordial germ cells, and protocols and best practices for toxicology and pH studies using echinoderms and several new chapters outlining the use of sea urchins in the classroom.

Key Features

  • Clear, concise protocols provided by experts who have established the echinoderms as a model system
  • Highlights new advances in the field, with this update presenting interesting chapters on echinoderms

Readership

New research scholars who wish to learn the echinoderms as a model system will find this book invaluable and for those already in the field, it will serve as a handy resource for the teaching and research laboratory


Details

No. of pages:
362
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128159545

Ratings and Reviews


About the Serial Volume Editors

Kathleen Foltz Serial Volume Editor

Kathy Foltz is a Professor in the Department of Molecular, Cellular and Developmental Biology at the University of California, Santa Barbara. She came to UCSB after her Postdoctoral work at SUNY- Stony Brook with William Lennarz following her PhD work at Purdue University with David Asai. A Searle Scholar, NSF Presidential Faculty Fellow and AAAS Fellow, she has used sea urchins, sea stars and other invertebrate deuterostomes to investigate questions of gamete recognition, egg activation and control of cell division throughout her career. She enjoys sharing her curiosity and knowledge with many undergraduates, graduate students and postdoctoral research colleagues. With the UCSB Marine Lab located on the main campus, over 1,000 undergraduate students have also worked with these fascinating organisms under her guidance in the Developmental Biology Laboratory classroom.

Affiliations and Expertise

Professor, Molecular, Cellular, and Developmental Biology, and the Marine Science Institute, University of California, Santa Barbara, CA. USA

Amro Hamdoun Serial Volume Editor

Amro Hamdoun is an Associate Professor in the Scripps Institution of Oceanography of the University of California, San Diego. His research bridges developmental biology and environmental toxicology, with a focus on the roles of xenobiotic transporters in the embryo. His research merges biochemical, cellular and structural approaches with high-resolution live imaging of echinoderm development. This work has been featured on the covers of Development, Developmental Dynamics, Molecular Biology of the Cell and Molecular Reproduction and Development. The Hamdoun laboratory is also a leading contributor of reagents for the sea urchin through Addgene (https://www.addgene.org/Amro_Hamdoun/). He was a recipient of the NIH Ruth L. Kirschstein NRSA and Pathway to Independence fellowships, and the Scripps Institution of Oceanography Outstanding Undergraduate Teaching Award.

Affiliations and Expertise

Amro Hamdoun is an Associate Professor at the University of California, San Diego, La Jolla, CA, USA