1st Edition - February 16, 2019
  • Editors: Kathleen Foltz, Amro Hamdoun
  • eBook ISBN: 9780128159552
  • Hardcover ISBN: 9780128159545

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Echinoderms, Volume 150 in the Methods in Cell Biology series, highlights new advances in the field, with this update presenting interesting chapters on procuring animals and culturing of eggs and embryos, cryopreservation of sea urchin gametes, emerging echinoderm models, culturing of sand dollars, cidaroids and heart urchins, culturing echinoderm larvae through metamorphosis, microinjection methods, injection of exogenous messages and protein overexpression, blastomere transplantation, visualization of embryonic polarity, larval immune cell approaches, methods for analysis of sea urchin primordial germ cells, and protocols and best practices for toxicology and pH studies using echinoderms and several new chapters outlining the use of sea urchins in the classroom.

Key Features

  • Clear, concise protocols provided by experts who have established the echinoderms as a model system
  • Highlights new advances in the field, with this update presenting interesting chapters on echinoderms


New research scholars who wish to learn the echinoderms as a model system will find this book invaluable and for those already in the field, it will serve as a handy resource for the teaching and research laboratory

Table of Contents

  • Section 1 Procurement and culturing of established and emerging echinoderm models
    1. Procuring animals and culturing of eggs and embryos
    Nikki L. Adams, Andreas Heyland, Linda L. Rice and Kathy R. Foltz
    2. Cryopreservation of sea urchin sperm and early life stages
    Estafania Paredes, Serean L. Adams and Julien Vignier
    3. Temnopleurus as an emerging echinoderm model
    Shunsuke Yaguchi
    4. Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods
    Taku Hibino, Takuya Minokawa and Atsuko Yamazaki
    5. The painted sea urchin, Lytechinus pictus, as a genetically-enabled developmental model
    Katherine T. Nesbit, Travis Fleming, Grant Batzel, Amara Pouv, Hannah Rosenblatt, Douglas A. Pace, Amro Hamdoun and Deirdre C. Lyons
    6. Culturing echinoderm larvae through metamorphosis
    Jason Hodin, Andreas Heyland, Annie Mercier, Bruno Pernet, David L. Cohen, Jean-François Hamel, Jonathan D. Allen, Justin S. McAlister, Maria Byrne, Paula Cisternas and Sophie B. George

    Section 2 Experimental embryology approaches
    7. Microinjection methods for sea urchin eggs and blastomeres
    Junko Yaguchi
    8. Microinjection of oocytes and embryos with synthetic mRNA encoding molecular probes
    George von Dassow, Jenna Valley and Kara Robbins
    9. Methods for transplantation of sea urchin blastomeres
    Andrew N. George and David R. McClay
    10. Sea urchin embryonic cilia
    Robert L. Morris and Victor D. Vacquier
    11. Visualizing egg and embryonic polarity
    Lauren T. Smith and Athula H. Wikramanayake
    12. Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs)
    Joseph P. Campanale, Amro Hamdoun, Gary M. Wessel, Yi-Hsien Su and Nathalie Oulhen
    13. Culture of and experiments with sea urchin embryo primary mesenchyme cells
    Bradley Moreno, Allessandra DiCorato, Alexander Park, Kellen Mobilia, Regina Knapp, Reiner Bleher, Charlene Wilke, Keith Alvares and Derk Joester

    Section 3 Approaches for assessing environmental influences on adults and embryos
    14. Analysis of immune response in the sea urchin larva
    Katherine M. Buckley, Nicholas W. Schuh, Andreas Heyland and Jonathan P. Rast
    15. Methods for collection, handling, and analysis of sea urchin coelomocytes
    L. Courtney Smith, Teresa S. Hawley, John H. Henson, Audrey J. Majeske, Matan Oren and Benyamin Rosental
    16. Measurement of feeding rates, respiration, and pH regulatory processes in the light of ocean acidification research
    Meike Stumpp, Sam Dupont and Marian Y. Hu
    17. Methods for toxicology studies in echinoderm embryos and larvae
    Cristina Torres-Duarte, Carol A. Vines, Elise Fairbairn and Gary N. Cherr

    Section 4 Sea urchins in the classroom
    18. A teaching laboratory on the activation of xenobiotic transporters at fertilization of sea urchins
    Lauren E. Shipp, Rose Z. Hill and Amro Hamdoun
    19. Exploring the sea urchin genome with undergraduates using bioinformatic tools
    Laura Romano, Christine Byrum, Pei Yun Lee and Robert Morris
    20. Analyzing gene expression in sea star eggs and embryos using bioinformatics
    Lauren Bates, Emily Wiseman and David J. Carroll

Product details

  • No. of pages: 504
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: February 16, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128159552
  • Hardcover ISBN: 9780128159545

About the Serial Volume Editors

Kathleen Foltz

Kathy Foltz is a Professor in the Department of Molecular, Cellular and Developmental Biology at the University of California, Santa Barbara. She came to UCSB after her Postdoctoral work at SUNY- Stony Brook with William Lennarz following her PhD work at Purdue University with David Asai. A Searle Scholar, NSF Presidential Faculty Fellow and AAAS Fellow, she has used sea urchins, sea stars and other invertebrate deuterostomes to investigate questions of gamete recognition, egg activation and control of cell division throughout her career. She enjoys sharing her curiosity and knowledge with many undergraduates, graduate students and postdoctoral research colleagues. With the UCSB Marine Lab located on the main campus, over 1,000 undergraduate students have also worked with these fascinating organisms under her guidance in the Developmental Biology Laboratory classroom.

Affiliations and Expertise

Professor, Molecular, Cellular, and Developmental Biology, and the Marine Science Institute, University of California, Santa Barbara, CA. USA

Amro Hamdoun

Amro Hamdoun is an Associate Professor in the Scripps Institution of Oceanography of the University of California, San Diego. His research bridges developmental biology and environmental toxicology, with a focus on the roles of xenobiotic transporters in the embryo. His research merges biochemical, cellular and structural approaches with high-resolution live imaging of echinoderm development. This work has been featured on the covers of Development, Developmental Dynamics, Molecular Biology of the Cell and Molecular Reproduction and Development. The Hamdoun laboratory is also a leading contributor of reagents for the sea urchin through Addgene ( He was a recipient of the NIH Ruth L. Kirschstein NRSA and Pathway to Independence fellowships, and the Scripps Institution of Oceanography Outstanding Undergraduate Teaching Award.

Affiliations and Expertise

Amro Hamdoun is an Associate Professor at the University of California, San Diego, La Jolla, CA, USA