Echinoderms Part B - 1st Edition - ISBN: 9780128170724

Echinoderms Part B, Volume 151

1st Edition

Serial Volume Editors: Amro Hamdoun Kathleen Foltz
Hardcover ISBN: 9780128170724
Imprint: Academic Press
Published Date: 1st April 2019
Page Count: 362
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Table of Contents

Section 1 - Methods for Genome and Transcriptome analysis
1. Echinoderm Genome Databases
Greg Cary, Veronica Hinman and Robert Andrew Cameron
2. Analysis of gene regulatory networks
Isabelle S. Peter
3. Using ATAC-seq and RNA-seq to increase resolution in GRN connectivity
Ina Arnone
4. Analysis of metabolomic and proteomic data
Greg Wray
5. Multiplex cis-regulatory Analysis
Jongmin Nam
6. Nanostring analysis of gene expression
Paola Oliveri
7. Experimental approaches GRN/signal pathways
Ryan Range
8. BACs
Kate Michele Buckley
9. Genome-Wide Analysis of Chromatin Accessibility Using ATAC-seq Tanvi Shashikant and Charles Ettensohn
Charles Ettensohn and Kate Michele Buckley

Section 2 - Genome-editing and proteomics
10. Injection of exogenous mRNAs to study gene function in echinoderm embryos
Thierry Lepage
11. Analysis of sea urchin proteins /Click IT
Michelle Marie Roux-Osovitz
12. CRISPR/Cas9-mediated genome editing in sea urchins
Yi-Hsien Su
13. scRNA and ChIP Analysis
Gary M. Wessel
14. Analysis of micro RNAs
Jia Song
15. Polysome isolation and methods for analysis of protein translation
Julia Morales
16. Generation, expression and utilization of single domain antibodies
Amro Hamdoun, Geoffrey Chang and Catherine Sarah Schrankel

Section 3 - Imaging of echinoderm embryos
17. Fluorescence and transmitted light microscopy
Charles Bradley Schuster
18. In toto imaging
Nadine Peyrarias
19. Isolation of the egg cortex for high resolution imaging
John Henson
20. Spatially mapping gene expression in sea urchin primary mesenchyme cells
Cynthia Bradham

Section 5 - Methods for measurement of intracellular signals in eggs, sperm and embryos
21. Calcium Imaging
Sandip Patel
22. Measuring ion and voltage gradients across the embryo
Cynthia Bradham
23. Analysis of sperm
Alberto Darszon
24. Kinetic and photonic techniques to study chemotatic signaling in urchin sperm
u. benjamin kaupp
25. Calcium sensors and imaging
Robert Burke


Description

Echinoderms, Volume 151, the latest release in the Methods in Cell Biology series, highlights advances in the field, with this update presenting chapters on

Echinoderm Genome Databases, an Analysis of gene regulatory networks, Using ATAC-seq and RNA-seq to increase resolution in GRN connectivity, an Analysis of metabolomic and proteomic data, Multiplex cis-regulatory analysis, Nanostring analysis of gene expression, Experimental approaches GRN/signal pathways, BACs, Genome-Wide Analysis of Chromatin Accessibility Using ATAC-seq Tanvi Shashikant and Charles Ettensohn, Injection of exogenous mRNAs to study gene function in echinoderm embryos, an Analysis of sea urchin proteins /Click IT, CRISPR/Cas9-mediated genome editing in sea urchins, and much more.

Key Features

  • Presents clear, concise protocols provided by experts who have established the echinoderms as a model systems
  • Highlights new advances in the field, with this update presenting interesting chapters on echinoderms

Readership

New research scholars who wish to learn the echinoderms as a model system will find this book invaluable and for those already in the field, it will serve as a handy resource for the teaching and research laboratory


Details

No. of pages:
362
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128170724

Ratings and Reviews


About the Serial Volume Editors

Amro Hamdoun Serial Volume Editor

Amro Hamdoun is Associate Professor of Biology in the Marine Biology Research Division at Scripps Institution of Oceanography, CA, USA

Affiliations and Expertise

Associate Professor of Biology, Marine Biology Research Division, Scripps Institution of Oceanography, CA, USA

Kathleen Foltz Serial Volume Editor

Kathy Foltz is a Professor in the Department of Molecular, Cellular and Developmental Biology at the University of California, Santa Barbara. She came to UCSB after her Postdoctoral work at SUNY- Stony Brook with William Lennarz following her PhD work at Purdue University with David Asai. A Searle Scholar, NSF Presidential Faculty Fellow and AAAS Fellow, she has used sea urchins, sea stars and other invertebrate deuterostomes to investigate questions of gamete recognition, egg activation and control of cell division throughout her career. She enjoys sharing her curiosity and knowledge with many undergraduates, graduate students and postdoctoral research colleagues. With the UCSB Marine Lab located on the main campus, over 1,000 undergraduate students have also worked with these fascinating organisms under her guidance in the Developmental Biology Laboratory classroom

Affiliations and Expertise

Associate Professor, Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA