Key Features

  • Broad-based up-to-date resource for the dynein class of molecular motors
  • Chapters written by world experts in their topics
  • Numerous well-illustrated figures and tables included to complement the text, imparting comprehensive information on dynein composition, interactions, and other fundamental features

Readership

Laboratory and clinical researchers across cell biology, developmental biology, genetics, protein chemistry, neurobiology, biophysics, biomedicine and medicine.

Table of Contents

List of Contributors

Preface

1. Discovery of Dynein and its Properties

1.1. Introduction

1.2. Research at Harvard

1.3. Research at the University of Hawaii

1.4. Semi-Retirement in Berkeley

1.5. The Pluses of Working on a Minus-End-Directed Motor

1.6. Epilogue

2. Evolutionary Biology of Dyneins

2.1. Introduction

2.2. Dynein Classification

2.3. Dynein Evolution in Eukaryotes

2.4. Evolution in the Proto-Eukaryote

2.5. The Origins of Dynein

2.6. Summary

3. The AAA+ Powerhouse – Trying to Understand How it Works

3.1. Introduction

3.2. Sequence analysis of the Dynein AAA+ Domains

3.3. Nucleotide Binding in the Motor Domain

3.4. How are the AAA+ Modules Spatially Arranged?

3.5. Communication of the Motor Domain with the Microtubule-Binding Domain

3.6. Transduction of Local Conformational Changes into Motion

3.7. Conclusion

4. Dynein Motor Mechanisms

4.1. The Dynein Engine Room

4.2. The Linker Arm and Powerstroke

4.3. Microtubule Affinity: Binding at A Distance

4.4. A Three-Part Harmony in A Big Block V-8

5. Structural Analysis of Dynein Intermediate and Light Chains

5.1. Introduction

5.2. Abbreviated Background of Light Chains

5.3. Structure of The Apo Light Chains

5.4. Structure of Liganded Light Chains

5.5. LC8 and Tctex1 Promiscuity

5.6. Light Chain Isoforms

5.7. Mammalian Dynein Intermediate Chains

5.8. Molecular Model of the Light Chain–Intermediate Chain Structure

5.9. Light Chains and Cargo

5.10. Post-Translational Modifications

5.11. The Roles of LC8 and Tctex1 on Dynein

5.12. Summary

6. Biophysics of Dynein In Vivo

6.1. Single-Molecule Properties of Dynein In Vitro

6.2. Multiple-Motor Prop

Details

No. of pages:
656
Language:
English
Copyright:
© 2011
Published:
Imprint:
Academic Press
Print ISBN:
9780123820044
Electronic ISBN:
9780123820051

About the author

Stephen King

Affiliations and Expertise

University of Texas, MD Anderson Cancer Center,Michale E. Keeling Center for Comparative Medicine and Research, Bastrop, Texas, U.S.A.