COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Drug and Enzyme Targeting, Part B - 1st Edition - ISBN: 9780121820497, 9780080882666

Drug and Enzyme Targeting, Part B, Volume 149

1st Edition

Editors in Chief: Nathan Colowick Nathan Kaplan
Serial Volume Editors: Ralph Green Kenneth Widder
eBook ISBN: 9780080882666
Imprint: Academic Press
Published Date: 19th November 1987
Page Count: 359


This product is currently not available for sale.

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

Cell Targeting Techniques: Biochemical Methods for the Study of Receptor-Mediated Endocytosis. High-Efficiency Gene Transfer into Cells. Lectin-Specific Targeting of Lysosomal Enzymes to Reticuloendothelial Cells. Osmotic Lysis of Pinosomes. Liposome Carriers: Multiple Emulsions as Targetable Delivery Systems. High-Pressure Continuous-Flow System for Drug Entrapment in Liposomes. Temperature- and pH-Sensitive Liposomes for Drug Targeting. pH-Sensitive Immunoliposomes. Photolabile Liposomes as Carriers. Covalent Attachment of Proteins to Liposomes. Use of Avidin-Biotin Technology for Liposome Targeting. Complement-Dependent Phagocytosis of Liposomes by Macrophages. Interaction of Liposomes with Cells. Uptake of Liposome-Encapsulated Agents. Liposome-Mediated Macrophage Activities. Liposomes as Carriers for in Vivo Gene Transfer and Expression. Liposome Entrapment for Delivery of Genetic Material to Plant Protoplasts. Liposome-Encapsulated Hemoglobin as an Artificial Oxygen-Carrying System. Use of Liposomes as Agglutination-Enhancement Agents in Diagnostic Tests. Cellular Carriers: Erythrosomes: Erythrocyte Cytoskeletons Coated with Exogenous Phospholipid as an Encapsulating System. Hypotonic Hemolysis Methods for Entrapment of Agents in Resealed Erythrocytes. High-Efficiency Entrapment of Enzymes in Resealed Red Cell Ghosts by Dialysis. Dialysis Method for Entrapment of Proteins into Resealed Red Blood Cells. Large-Scale Entrapment of Drugs into Resealed Red Blood Cells Using a Continuous-Flow Dialysis System. Electric Modification of Membrane Permeability for Drug Loading into Living Cells. Drug-Induced Endocytosis and Entrapment in Red Cells and Ghosts. Preparation of White Resealable Erythrocyte Ghosts. Phospholipid Asymmetry of Loaded Red Cells. Red Cell-Mediated Microinjection. Magnetically Responsive Erythrocyte "Ghosts". Use of Platelets as Drug Carriers for the Treatment of Hematologic Diseases. Author Index. Subject Index.


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, pharmacologists, toxicologists, analytical chemists, and biomedical researchers.


No. of pages:
© Academic Press 1987
19th November 1987
Academic Press
eBook ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

Nathan Colowick

Affiliations and Expertise

Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.

Nathan Kaplan

About the Serial Volume Editors

Ralph Green

Affiliations and Expertise

The Clevland Clinic Foundation, Ohio, U.S.A.

Kenneth Widder

Affiliations and Expertise

Molecular Biosystems, Inc. and University of California, San Diego, U.S.A.