Cell Targeting Techniques: Biochemical Methods for the Study of Receptor-Mediated Endocytosis. High-Efficiency Gene Transfer into Cells. Lectin-Specific Targeting of Lysosomal Enzymes to Reticuloendothelial Cells. Osmotic Lysis of Pinosomes.
Liposome Carriers: Multiple Emulsions as Targetable Delivery Systems. High-Pressure Continuous-Flow System for Drug Entrapment in Liposomes. Temperature- and pH-Sensitive Liposomes for Drug Targeting. pH-Sensitive Immunoliposomes. Photolabile Liposomes as Carriers. Covalent Attachment of Proteins to Liposomes. Use of Avidin-Biotin Technology for Liposome Targeting. Complement-Dependent Phagocytosis of Liposomes by Macrophages. Interaction of Liposomes with Cells. Uptake of Liposome-Encapsulated Agents. Liposome-Mediated Macrophage Activities. Liposomes as Carriers for in Vivo Gene Transfer and Expression. Liposome Entrapment for Delivery of Genetic Material to Plant Protoplasts. Liposome-Encapsulated Hemoglobin as an Artificial Oxygen-Carrying System. Use of Liposomes as Agglutination-Enhancement Agents in Diagnostic Tests.
Cellular Carriers: Erythrosomes: Erythrocyte Cytoskeletons Coated with Exogenous Phospholipid as an Encapsulating System. Hypotonic Hemolysis Methods for Entrapment of Agents in Resealed Erythrocytes. High-Efficiency Entrapment of Enzymes in Resealed Red Cell Ghosts by Dialysis. Dialysis Method for Entrapment of Proteins into Resealed Red Blood Cells. Large-Scale Entrapment of Drugs into Resealed Red Blood Cells Using a Continuous-Flow Dialysis System. Electric Modification of Membrane Permeability for Drug Loading into Living Cells. Drug-Induced Endocytosis and Entrapment in Red Cells and Ghosts. Preparation of White Resealable Erythrocyte Ghosts. Phospholipid Asymmetry of Loaded Red Cells. Red Cell-Mediated Microinjection. Magnetically Responsive Erythrocyte "Ghosts". Use of Platelets as Drug Carriers for the Treatment of Hematologic Diseases. Author Index. Subject Index.
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Biochemists, pharmacologists, toxicologists, analytical chemists, and biomedical researchers.
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- © Academic Press 1987
- 19th November 1987
- Academic Press
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Molecular Biosystems, Inc. and University of California, San Diego, U.S.A.
Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.