Drosophila Cells in Culture - 2nd Edition - ISBN: 9780128094730

Drosophila Cells in Culture

2nd Edition

Authors: Guy Echalier Norbert Perrimon Stephanie Mohr
Paperback ISBN: 9780128094730
Imprint: Academic Press
Published Date: 8th December 2017
Page Count: 258
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Description

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.

Key Features

  • Offers full coverage of cell lines and primary cultures
  • Provides a go-to resource for methods and studies completed with drosophila cells in culture
  • Presents a wide spectrum of experimental techniques

Readership

Early researchers, graduate students, investigators studying developmental biology, genetics, neuroscience, biochemistry and across the biological and biomedical sciences

Table of Contents

1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells
2. Primary Cell Cultures of Drosophila Embryonic Cells
3. Continuous Drosophila Cell Lines Established In Vitro
4. Cells or Tissues In Course of Differentiation
5. Hormonal Control of The Development of Insects: Implications In Organ or Cell Cultures of Drosophila
6. Gene Transfer into Cultured and Applications
7. Rnai and Crispr (Cas9, Cas9-Act, Cas9-Repressor) Methods to Perturb Gene Function
8. Functional Genomics Screens Using Rnai, Crispr, and Orf Overexpression

Details

No. of pages:
258
Language:
English
Copyright:
© Academic Press 2018
Published:
Imprint:
Academic Press
Paperback ISBN:
9780128094730

About the Author

Guy Echalier

Guy Echalier is an esteemed expert in the research of Drosophilia, having over thirty years experience handling in vitro cultured Drosophilia cells.

Affiliations and Expertise

Center National de la Recherche Scientifique, Universite Pierre et Marie Curie, Paris, France

Norbert Perrimon

Norbert Perrimon is a leading researcher in developmental biology. His current research pertains to using Drosophilia as a model system to characterize the responses of cells to external signalling.

Affiliations and Expertise

Department of Genetics, Harvard Medical School

Stephanie Mohr

Stephanie E. Mohr is a leader in the field of high-throughput functional genomics screening in Drosophila cultured cells.

Affiliations and Expertise

Department of Genetics, Harvard Medical School