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DNA Structures, Part B, Chemical and Electrophoretic Analysis of DNA - 1st Edition - ISBN: 9780121821135, 9780080883250

DNA Structures, Part B, Chemical and Electrophoretic Analysis of DNA, Volume 212

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: David Lilley James Dahlberg
eBook ISBN: 9780080883250
Imprint: Academic Press
Published Date: 4th August 1992
Page Count: 501


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Table of Contents

Gel Electrophoresis and Topological Methods for Analysis of DNA Structure: D.M. Crothers, J. Drak, J.D. Kahn, and S.D. Levene, DNA Bending, Flexibility, and Helical Repeat by Cyclization Kinetics. S. Diekmann, Analyzing DNA Curvature in Polyacrylamide Gels. D.M. Crothers and J. Drak, Global Features of DNA Structure by Comparative Gel Electrophoresis. E.S. Abrams and V.P. Stanton, Jr. , Use of Denaturing Gradient Gel Electrophoresis to Study Conformational Transitions in Nucleic Acids. R. Bowater, F. Aboul-Ela, and D.M.J. Lilley, Two-Dimensional Gel Electrophoresis of Circular DNA Topoisomers. P. Dradoge and N.R. Cozzarelli, Topological Structures of DNA Knots and Catenanes. Probing DNA Structure in Vitro: D.M.J. Lilley, Probes of DNA Structure. E. Palecek, Probing DNA Structure with Osmium Tetroxide Complexes in Vitro. T. Kohwi-Shigematsu and Y. Kohwi, Detection of Non-B-DNA Structures at Specific Sites in Supercoiled Plasmid DNA and Chromatin with Haloacetaldehyde and Diethyl Pyrocarbonate. B.H. Johnston, Hydroxylamine and Methoxylamine as Probes of DNA Structure. M.A. Price and T.D. Tullius, Using Hydroxyl Radical to Probe DNA Structure. C.S. Chow and J.K. Barton, Transition Metal Complexes as Probes of Nucleic Acids. D.W. Ussery, R.W. Hoepfner, and R.R. Sinden, Probing DNA Structure With Psoralen in Vitro. M.M. Becker and G. Grossmann, Photofootprinting DNA in Vitro. H. Htun and B.H. Johnston, Mapping Adducts of DNA Structural Probes Using Transcription and Primer Extension Approaches. F. Wohlrab, Enzyme Probes in Vitro. Analysis of DNA Structure Inside Cells: E. Palecek, Probing of DNA Structure in Cells with Osmium Tetroxide-2,2'*bBipyridine. R.R. Sinden and D.W. Ussery, Analysis of DNA Structure in Vivo Using Psoralen Photobinding: Measurement of Supercoiling, Topological Domains, and DNA-Protein Interactions. W. Zacharias, DNA Methylation in Vivo. L.F. Liu and H.-Y. Wu, Topological Approaches to Studies of Protein-Mediated Looping of DNA in Vivo. Interaction of DNA and Proteins: D.G. Sanford and B.D. Stollar, Assay of Anti-DNA Antibodies. P. Dradoge and A. Nordheim, Topoisomer Gel Retardation: Protein Recognition of Torsional Stress-Induced DNA Conformations. W.G. Turnell and A.A. Travers, Algorithms for Prediction of Histone Octamer Binding Sites. T.M. Lohman and D.P. Mascotti, Thermodynamics of Ligand-Nucleic Acid Interactions. T.M. Lohman and D.P. Mascotti, Nonspecific Ligand-DNA Equilibrium Binding Parameters Determined by Fluorescence Methods. Author Index. Subject Index.


To understand the structure and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 211 of Methods of Enzymology, collate in one source all the methods and approaches needed to achieve this goal.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.


No. of pages:
© Academic Press 1992
4th August 1992
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

David Lilley

Affiliations and Expertise

The University, Dundee, Scotland

James Dahlberg

Affiliations and Expertise

University of Wisconsin, Madison, U.S.A.