Description

To understand the structure and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 211 of Methods of Enzymology, collate in one source all the methods and approaches needed to achieve this goal.

Readership

Biochemists, biophysicists, physical chemists, and molecular and cell biologists.

Table of Contents

Gel Electrophoresis and Topological Methods for Analysis of DNA Structure: D.M. Crothers, J. Drak, J.D. Kahn, and S.D. Levene, DNA Bending, Flexibility, and Helical Repeat by Cyclization Kinetics. S. Diekmann, Analyzing DNA Curvature in Polyacrylamide Gels. D.M. Crothers and J. Drak, Global Features of DNA Structure by Comparative Gel Electrophoresis. E.S. Abrams and V.P. Stanton, Jr. , Use of Denaturing Gradient Gel Electrophoresis to Study Conformational Transitions in Nucleic Acids. R. Bowater, F. Aboul-Ela, and D.M.J. Lilley, Two-Dimensional Gel Electrophoresis of Circular DNA Topoisomers. P. Dr~adoge and N.R. Cozzarelli, Topological Structures of DNA Knots and Catenanes. Probing DNA Structure in Vitro: D.M.J. Lilley, Probes of DNA Structure. E. Palecek, Probing DNA Structure with Osmium Tetroxide Complexes in Vitro. T. Kohwi-Shigematsu and Y. Kohwi, Detection of Non-B-DNA Structures at Specific Sites in Supercoiled Plasmid DNA and Chromatin with Haloacetaldehyde and Diethyl Pyrocarbonate. B.H. Johnston, Hydroxylamine and Methoxylamine as Probes of DNA Structure. M.A. Price and T.D. Tullius, Using Hydroxyl Radical to Probe DNA Structure. C.S. Chow and J.K. Barton, Transition Metal Complexes as Probes of Nucleic Acids. D.W. Ussery, R.W. Hoepfner, and R.R. Sinden, Probing DNA Structure With Psoralen in Vitro. M.M. Becker and G. Grossmann, Photofootprinting DNA in Vitro. H. Htun and B.H. Johnston, Mapping Adducts of DNA Structural Probes Using Transcription and Primer Extension Approaches. F. Wohlrab, Enzyme Probes in Vitro.

Details

No. of pages:
501
Language:
English
Copyright:
© 1992
Published:
Imprint:
Academic Press
Print ISBN:
9780121821135
Electronic ISBN:
9780080883250

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY