DNA Structures, Part B, Chemical and Electrophoretic Analysis of DNA - 1st Edition - ISBN: 9780121821135, 9780080883250

DNA Structures, Part B, Chemical and Electrophoretic Analysis of DNA, Volume 212

1st Edition

Serial Volume Editors: David Lilley James Dahlberg
Editor-in-Chiefs: John Abelson Melvin Simon
eBook ISBN: 9780080883250
Imprint: Academic Press
Published Date: 4th August 1992
Page Count: 501


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Table of Contents

Gel Electrophoresis and Topological Methods for Analysis of DNA Structure:

D.M. Crothers, J. Drak, J.D. Kahn, and S.D. Levene, DNA Bending, Flexibility, and Helical Repeat by Cyclization Kinetics.

S. Diekmann, Analyzing DNA Curvature in Polyacrylamide Gels.

D.M. Crothers and J. Drak, Global Features of DNA Structure by Comparative Gel Electrophoresis.

E.S. Abrams and V.P. Stanton, Jr. , Use of Denaturing Gradient Gel Electrophoresis to Study Conformational Transitions in Nucleic Acids.

R. Bowater, F. Aboul-Ela, and D.M.J. Lilley, Two-Dimensional Gel Electrophoresis of Circular DNA Topoisomers.

P. Dr~adoge and N.R. Cozzarelli, Topological Structures of DNA Knots and Catenanes.

Probing DNA Structure in Vitro:

D.M.J. Lilley, Probes of DNA Structure.

E. Palecek, Probing DNA Structure with Osmium Tetroxide Complexes in Vitro.

T. Kohwi-Shigematsu and Y. Kohwi, Detection of Non-B-DNA Structures at Specific Sites in Supercoiled Plasmid DNA and Chromatin with Haloacetaldehyde and Diethyl Pyrocarbonate.

B.H. Johnston, Hydroxylamine and Methoxylamine as Probes of DNA Structure.

M.A. Price and T.D. Tullius, Using Hydroxyl Radical to Probe DNA Structure.

C.S. Chow and J.K. Barton, Transition Metal Complexes as Probes of Nucleic Acids.

D.W. Ussery, R.W. Hoepfner, and R.R. Sinden, Probing DNA Structure With Psoralen in Vitro.

M.M. Becker and G. Grossmann, Photofootprinting DNA in Vitro.

H. Htun and B.H. Johnston, Mapping Adducts of DNA Structural Probes Using Transcription and Primer Extension Approaches.

F. Wohlrab, Enzyme Probes in Vitro.<BR i


To understand the structure and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 211 of Methods of Enzymology, collate in one source all the methods and approaches needed to achieve this goal.


Biochemists, biophysicists, physical chemists, and molecular and cell biologists.


No. of pages:
© Academic Press 1992
Academic Press
eBook ISBN:


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About the Serial Volume Editors

David Lilley Serial Volume Editor

Affiliations and Expertise

The University, Dundee, Scotland

James Dahlberg Serial Volume Editor

Affiliations and Expertise

University of Wisconsin, Madison, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA