Description

To understand the structures and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 212 of Methods in Enzymology, collate in one source all the methods and approaches needed to achieve this goal.

Readership

Biochemists, biophysicists, molecular biologists, microbiologists, geneticists, and industrial researchers working on protein products.

Table of Contents

Chemical Synthesis of DNA: M.H. Caruthers, G. Beaton, J.V. Wu, and W. Wiesler, Chemical Synthesis of Deoxyoligonucleotides and Deoxyoligonucleotide Analogs. T. Brown and D.J.S. Brown, Purification of Synthetic DNA. B.A. Connolly, Synthetic Oligodeoxynucleotides Containing Modified Bases. P.S. Miller, Preparation of Psoralen-Derivatized Oligodeoxyribonucleodise Methylphosphonates. Nonstandard DNA Structures and Their Analysis: R.E. Dickerson, DNA Structure from A to Z. V.I. Ivanov and D.Y. Krylov, A-DNA in Solution as Studied by Diverse Approaches. B.H. Johnston, Generation and Detection of Z-DNA. A.H. Murchie and D.M.J. Lilley, Supercoiled DNA and Cruciform Structures. M.D. Frank-Kamenetskii, Protonated DNA Structures. D. Sen and W. Gilbert, Guanine Quartet Structures. K. Rippe and T.M. Jovin, Parallel-Stranded Duplex DNA. W.N. Hunter, Crystallographic Studies of DNA Containing Mismatches, Modified, and Unpaired Bases. Spectroscopic Methods for Analysis of DNA: J. Feigon, V. Sklenar, E. Wang, D.E. Gilbert, R.F. Macaya, and P. Schultze, 1H NMR Spectroscopy of DNA. D.G. Gorenstein, 31P NMR of DNA. J.Van Wijk, B.D. Huckriede, J.H. Ippel, and C. Altona, Furanose Sugar Conformations in DNA from NMR Coupling Constants. E. Taillandier and J. Liquier, Infrared Spectroscopy of DNA. W.L. Peticolas and E. Evertsz, Conformation of DNA in Vitro and in Vivo from Laser Raman Scattering. R.M. Clegg, Fluorescence Resonance Energy Transfer and Nucleic Acids. D.M. Gray, R.L. Ratliff, an

Details

No. of pages:
619
Language:
English
Copyright:
© 1992
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883243
Print ISBN:
9780121821128

About the serial-volume-editors

David Lilley

Affiliations and Expertise

The University, Dundee, Scotland

James Dahlberg

Affiliations and Expertise

University of Wisconsin, Madison, U.S.A.

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY