DNA Sensors and Inflammasomes - 1st Edition - ISBN: 9780128183595

DNA Sensors and Inflammasomes, Volume 625

1st Edition

Serial Volume Editors: Jungsan Sohn
Hardcover ISBN: 9780128183595
Imprint: Academic Press
Published Date: 1st September 2019
Page Count: 412
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Table of Contents

1. Phosphorylation and dimerization of STING and IRF3
Søren R. Paludan
2. cGAS enzymology
Philip J. Kranzusch
3. Synthesis and identification of immuno-stimulatory CDNs
Jörg S. Hartig
4. Tracking cGAS activity/ cGAMP formation using SPR/NMR
Erik C. Ralph
5. Using an enzyme coupled assay to track cGAS activity under steady states
Jungsan Sohn
6. Tracking the polymerization of DNA sensors, inflammasome receptors, and downstream signaling partners using FRET
Jungsan Sohn
7. NLRC4 structure
Hao Wu
8. Tracking TREX1 activity
Fred W. Perrino
9. DNA association and dissociation kinetics of PARP1
Johannes Rudolph
10. Using AFM to track DNA: sensor complex formation
Alexey Krasnoslobodtsev
11. Using electron microscopy methods for visualizing DNA: sensor and inflammasome complexes (prep for cryo-EM)
Jungsan Sohn
12. Methods for cryo-EM sample prep/ reconstruction of the NAIP•NLRC4 complex
Eva Nogales
13. Innovative methods that underlie the solid state NMR studies of the inflammasome
Sebastian Hiller
14. Using NMR for probing ASC-ASC interaction
Eva de Alba
15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy
Brendan Antiochos
16. Monitoring IL-1B and IFN-1 output levels NLRP3 and cGAS agonists
Michael A. Chattergoon
17. ATM and inflammasome assembly
Nelson O. Gekara
18. STING/TBK
Leonie Unterholzner
19. cGAS and mitotic cell death
Nelson O. Gekara
20. Tracking PTMs of DNA sensors
Ileana M. Cristea
21. Ubiquitination
Nelson O. Gekara


Description

DNA Sensors and Inflammasomes, Volume 625, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this release include Phosphorylation and dimerization of STING and IRF3, cGAS enzymology, Synthesis and identification of immuno-stimulatory CDNs, Tracking cGAS activity/ cGAMP formation using SPR/NMR, Using an enzyme coupled assay to track cGAS activity under steady states, Tracking the polymerization of DNA sensors, inflammasome receptors, and downstream signaling partners using FRET, NLRC4 structure, Tracking TREX1 activity, DNA association and dissociation kinetics of PARP1, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on DNA sensors and inflammasomes

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128183595

Ratings and Reviews


About the Serial Volume Editors

Jungsan Sohn Serial Volume Editor

Jungsan Sohn is at Johns Hopkins School of Medicine, MD, USA

Affiliations and Expertise

Johns Hopkins School of Medicine, MD, USA