DNA Sensors and Inflammasomes

1st Edition, Volume 625 - August 25, 2019
Editor: Jungsan Sohn
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 8 1 8 3 5 9 - 5
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 1 8 3 6 0 - 1

DNA Sensors and Inflammasomes, Volume 625, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by le… Read more

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DNA Sensors and Inflammasomes, Volume 625, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New sections in this release include Phosphorylation and dimerization of STING and IRF3, cGAS enzymology, Synthesis and identification of immuno-stimulatory CDNs, Tracking cGAS activity/ cGAMP formation using SPR/NMR, Using an enzyme coupled assay to track cGAS activity under steady states, Tracking the polymerization of DNA sensors, inflammasome receptors, and downstream signaling partners using FRET, NLRC4 structure, Tracking TREX1 activity, DNA association and dissociation kinetics of PARP1, and more.

1. Intercellular communication in the innate immune system through the cGAS-STING pathway
Ramya Nandakumar, Samuel Joseph Windross and Søren R. Paludan

2. Analysis of human cGAS activity and structure
Wen Zhou, Aaron T. Whiteley and Philip J. Kranzusch

3. Chemical synthesis, purification, and characterization of 3′-5′-linked canonical cyclic dinucleotides (CDNs)
Changhao Wang, Min Hao, Qianqian Qi, Yashao Chen and Jörg S. Hartig

4. An SPR-based analysis of cGAS substrate K_D and steady-state K_M values
Erik C. Ralph and Justin Hall

5. A pyrophosphatase-coupled assay to monitor the NTase activity of cGAS
Richard Hooy and Jungsan Sohn

6. Tracking the polymerization of DNA sensors and inflammasomes using FRET
Zachary Mazanek and Jungsan Sohn

7. Monitoring gasdermin pore formation in vitro
Shiyu Xia, Jianbin Ruan and Hao Wu

8. Measuring TREX1 and TREX2 exonuclease activities
Wayne O. Hemphill and Fred W. Perrino

9. Kinetics of DNA: Protein association and dissociation by stopped-flow spectroscopy
Johannes Rudolph and Karolin Luger

10. Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imaging
Alexander Lushnikov, Richard Hooy, Jungsan Sohn and Alexey Krasnoslobodtsev

11. Preparation of filamentous proteins for electron microscopy visualization and reconstruction
Mariusz Matyszewski and Jungsan Sohn

12. Cryo-EM studies of NAIP–NLRC4 inflammasomes
Nicole Haloupek, Patricia Grob, Jeannette Tenthorey, Russell E. Vance and Eva Nogales

13. An integrative protocol for the structure determination of the mouse ASC-PYD filament
Adam Mazur, Petr Broz and Sebastian Hiller

14. Protein interactions of the inflammasome adapter ASC by solution NMR
Pedro Diaz-Parga and Eva de Alba

15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopy
Brendan Antiochos

16. Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPS
Guido Massaccesi and Michael A. Chattergoon

17. Biochemical and microscopic analysis of inflammasome complex formation
Patrycja Swacha, Nelson Ong’ondo Gekara and Saskia F. Erttmann

18. Comet and micronucleus assays for analyzing DNA damage and genome integrity
Hui Jiang and Nelson Ong'ondo Gekara

19. Workflows and considerations for investigating protein interactions of viral DNA sensors
Timothy R. Howard, Bokai Song and Ileana M. Cristea

20. TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexes
Swarupa Panda and Nelson Ong'ondo Gekara