The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Includes descriptions of functional, structural, kinetic, and genetic methods for analyzing major enzymes of DNA replication * Describes strategies for studying interactions of these proteins during replication * Provides comprehensive descriptions of uses of prokaryotic and eukaryotic crude in vitro replication systems and reconstitution of such systems from purified proteins * Includes methods for analyzing DNA replication in vivo


Biochemists, molecular biologists, biophysicists, cell biologists, biomedical researchers, microbiologists, analytical chemists, industrial researchers working on protein products, and geneticists.

Table of Contents

Purification and Characterization of DNA Polymerases: C.M. Joyce and V. Derbyshire, Purification of Escherichia coli DNA Polymerase I and Klenow Fragment. H. Cai, H. Yu, K. McEntee, and M.F. Goodman, Purification and Properties of DNA Polymerase II from Escherichia coli. M.G. Cull and C.S. McHenry, Purification of Escherichia coli DNA Polymerase III Holoenzyme. M.H. Barnes and N.C. Brown, Purification of DNA Polymerase III of Gram-Positive Bacteria. J.M. Lazaro, L. Blanco, and M. Salas, Purification of Bacteriophage phi29 DNA Polymerase. P.M.J. Burgers, DNA Polymerases from Saccharomyces cerevisiae. P.G. Mitsis, C.-S. Chiang, and I.R. Lehman, Purification of DNA Polymerase-Primase (DNA Polymerase alpha) and DNA Polymerase delta from Embryos of Drosophila melanogaster. T.S.-F. Wang, W.C. Copeland, L. Rogge, and Q. Dong, Purification of Mammalian DNA Polymerases: DNA Polymerase alpha. K.M. Downey and A.G. So, Purification of Mammalian Polymerases: DNA Polymerase delta. G.S.-J. Chui and S. Linn, Purification of Mammalian Polymerases: DNA Polymerase epsilon. W.A. Beard and S.H. Wilson, Purification and Domain-Mapping of Mammalian DNA Polymerasebeta. M.E. Budd and J.L. Campbell, Purification and Enzymatic and Functional Characterization of DNA Polymerase beta-like Enzyme POL4, Expressed during Yeast Meiosis. S.F.J. Le Grice, C.E. Cameron, and S.J. Benkovic, Purification and Characterization of Human Immunodeficiency Virus Type 1 Reverse Transcriptase. Structural Analysis of DNA Polymerases: G.P. Mullen, Solution Structure of DNA Polymerases and DNA Polymerase-Substrate Complexes.


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© 1995
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY