This volume provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair.

Key Features

*A laboratory standard for more than 40 years *Over 400 volumes strong *Also available on ScienceDirect *Part A of a 2-part series


Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Table of Contents

Table of Contents Chapter 1 Assays for determining lesion bypass efficiency and mutagenicity of site-specific DNA lesions in vivo Chapter 2 Oxidative DNA glycolases: Recipes from Cloning to Characterization Chapter 3 Purification and Characterization of NEIL1 and NEIL2, Members of a distinct Family of Mammalian DNA Glycosylases for repair of oxidized bases Chapter 4 Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-links Chapter 5 Isolation and analyses of MutY and MutY Homologs (MYH) Chapter 6 Use of yeast for detection of endogenous abasic legions, their source and their repair Chapter 7 Activities and Mechanism of Pol Beta Chapter 8 Direct removal of aklylation damage from DNA by AlkB and related DNA dioxygenases Chapter 9 Purification and Characterization of DNA Photolyases Chapter 10 Genetic and in vitro assays of DNA deamination Chapter 11 The XPC complex and UV-DDB: Functional assays for the damage recognition factors involved in global genome repair Chapter 12 Purification and Characterization of E. coli and Human Nucleotide Excision Repair Chapter 13 Assays for transcription elongation by RNA polymerase II using oligo (dC)-tailed template with single DNA damage Chapter 14 In vivo assays for transcription-coupled repair Chapter 15 TFIIh enzymatic activities in transcription and nucleotide excision repair Chapter 16 An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extracts


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© 2006
Academic Press
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