Diagnostic Molecular Pathology

1st Edition

A Guide to Applied Molecular Testing

Authors:

  • Gregory Tsongalis
  • William Coleman
  • Diagnostic Molecular Pathology

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    Description

    Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing is organized around disease types (genetic disease, infectious disease, neoplastic disease, among others). In each section, the authors provide background on disease mechanisms and describe how laboratory testing is built on knowledge of these mechanisms. Sections are dedicated to general methodologies employed in testing (to convey the concepts reflected in the methods), and specific description of how these methods can be applied and are applied to specific diseases are described.

    The book does not present molecular methods in isolation, but considers how other evidence (symptoms, radiology or other imaging, or other clinical tests) is used to guide the selection of molecular tests or how these other data are used in conjunction with molecular tests to make diagnoses (or otherwise contribute to clinical workup). In addition, final chapters look to the future (new technologies, new approaches) of applied molecular pathology and how discovery-based research will yield new and useful biomarkers and tests.

    Diagnostic Molecular Pathology contains exercises to test readers on their understanding of how molecular diagnostic tests are utilized and the value of the information that can be obtained in the context of the patient workup. Some of these exercises can be found at the end of each chapter in the form of questions and answers. Readers are also directed to an ancillary website that contains supplementary materials in the form of exercises where decision trees can be employed to simulate actual clinical decisions (with addition of time and cost of diagnosis).

    Key Features

    • Focuses on the menu of molecular diagnostic tests available in modern molecular pathology or clinical laboratories that can be applied to disease detection, diagnosis, and classification in the clinical workup of a patient
    • Explains how molecular tests are utilized to guide the treatment of patients in personalized medicine (guided therapies) and for prognostication of disease
    • Features an ancillary website with videos of laboratory tests being performed as well as self-testing exercises where decision trees can be employed to simulate actual clinical decisions
    • Highlights new technologies and approaches of applied molecular pathology and how discovery-based research will yield new and useful biomarkers and tests

    Readership

    Medical fellows in pathology and laboratory medicine, clinical pathologists, clinical laboratory scientists, and clinicians across internal medicine subspecialties, as well as biomedical science graduate students

    Table of Contents

    Section 1: Introduction to Molecular Testing

    1. Basic Concepts in Molecular Pathology – Introduction to Molecular Testing in Human Disease

      William B. Coleman and Gregory J. Tsongalis

    2. Laboratory Approaches in Molecular Pathology – Polymerase Chain Reaction and Other Amplification Techniques

      William B. Coleman and Gregory J. Tsongalis

    3. Laboratory Approaches in Molecular Pathology – Next Generation Sequencing

      Nirali M. Patel and Daniel L. Duncan

    4. Laboratory Approaches in Molecular Pathology – Automation in the Molecular Pathology Laboratory

      Gregory J. Tsongalis and Scot Turner

    Section 2:Molecular Testing in Infectious Disease

    5. Molecular Testing for Human Immunodeficiency Virus (HIV)

      Bruno Pozzetto

    6. Molecular Testing in Hepatitis Virus-related Disease (HBV, HCV)

      Tomasz I. Michalak

    7. Molecular Testing for Human Papilloma Viruses

      Katie M. Bennett

    8. Molecular Testing for Herpes Viruses

      Benjamin A. Pinsky

    9. Molecular Testing for Parvoviruses

      Giorgio Gallinella

    10. Molecular Testing for Polyomaviruses

      Belinda Yen-Lieberman and Gary W. Procop

    11. Molecular Testing for Respiratory Viruses

      Kathleen A. Stellrecht

    12. Molecular Testing for Diseases Associated with Bacterial Infection

      Melissa Miller

    13. Infectious Agents Associated with Sexually-transmitted Diseases

      Bruno Pozzetto

    14. Infectious Agents Associated with Nosocomial Infection (MRSA, C. difficile)

      Rodney Climaco Arcenas

    15. Molecular Testing in Emerging Infectious Diseases

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    Details

    No. of pages:
    578
    Language:
    English
    Copyright:
    © 2017
    Imprint:
    Academic Press
    Print ISBN:
    9780128008867
    Electronic ISBN:
    9780128011577

    About the authors

    Gregory Tsongalis

    Gregory J. Tsongalis, Ph.D., H.C.L.D., C.C. is Professor of Pathology, Director, Molecular Pathology, and Co-Director Translational Research Program, Department of Pathology, Dartmouth Hitchcock Medical Center and The Audrey and Theodor Geisel School of Medicine at Dartmouth, Lebanon, NH, USA

    William Coleman

    William B. Coleman, PhD is Professor of Pathology and Laboratory Medicine and Director of Graduate Studies for the Molecular and Cellular Pathology Graduate Program at the University of North Carolina School of Medicine (Chapel Hill, NC). Dr. Coleman is co-director of the UNC Program in Translational Medicine, and co-director of the Environmental Pathology Predoctoral and Postdoctoral Training Program. In addition, he is affiliated with the Curriculum in Toxicology and is a member of the UNC Lineberger Comprehensive Cancer Center. Dr. Coleman is actively involved in teaching biomedical graduate students and is a three-time recipient of the Joe W. Grisham Award for Excellence in Graduate Student Teaching from the Molecular and Cellular Pathology graduate students at UNC-Chapel Hill. Dr. Coleman is active in the leadership of the American Society for Investigative Pathology, and is a member of the American Association for Cancer Research. He serves on the editorial boards of Clinica Chimica Acta, The American Journal of Pathology, Experimental and Molecular Pathology, Archives of Pathology and Laboratory Medicine, and Laboratory Investigation, and has served as an ad hoc reviewer for 48 other journals. Dr. Coleman’s major research interests are in the molecular pathogenesis of human cancers, with a specific interest in breast cancer epigenetics, liver carcinogenesis, and lung cancer biology. His research has been funded by the NIH/NCI, The Susan G. Komen Breast Cancer Foundation, Friends for an Earlier Breast Cancer Test, and the UNC Lineberger Comprehensive Cancer Center. Dr. Coleman is the author of over 100 original research articles, reviews, and book chapters. In addition, Dr. Coleman has co-edited or co-authored six books on topics related to molecular pathology, molecular diagnostics, and the molecular pathogenesis of human cancer.