Diagnostic Molecular Biology

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to aid in the comprehension of this complex subject. Each technique described in this book is explained within its conceptual framework to enhance understanding, and current applications of clinical laboratory techniques are covered in detail.

The targeted approach covers the principles of molecular biology including the basic knowledge of nucleic acids, proteins, and genomes; the basic techniques and instrumentations that are often used in the field of molecular biology with detailed procedures and explanations; and the applications of the principle and techniques currently employed in the clinical laboratory.

• Provides an understanding of what techniques are used to diagnosis at the molecular level
• Explains how to use information technology to communicate and assess results in the lab
• Places protocols into context with practical applications

Researchers and technicians in the lab, pathologists, and students in molecular biology and microbiology

Part I
1. Introduction
2. Genes
3. Nucleic acids based cellular activities
4. Genes encode polypeptides
5. Genome

Part II
6. Extraction and purification of nucleic acids and proteins
7. Detection and analysis of nucleic acids and proteins
8. Characterization of nucleic acids and proteins
9. Amplification of nucleic acids
10. Genes and genomes sequencing techniques
11. Techniques in studying gene expression and function
12. Techniques in studying genomes

Part III
13. Techniques in human identification
14. Molecular diagnosis of mutation and inherited diseases
15. Molecular diagnosis of microorganisms and infectious diseases
16. Molecular diagnosis of cancers
17. Safety and quality management in clinical medical laboratory