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Cytometry, Part B - 3rd Edition - ISBN: 9780125441674, 9780080859620

Cytometry, Part B, Volume 64

3rd Edition

Serial Editors: Leslie Wilson Paul Matsudaira
Serial Volume Editors: Zbigniew Darzynkiewicz J. Paul Robinson Harry Crissman
Hardcover ISBN: 9780125441674
eBook ISBN: 9780080859620
Imprint: Academic Press
Published Date: 16th October 2000
Page Count: 614


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Table of Contents

Contents of Volume 63. Contributors. Preface to the Third Edition. Preface to the Second Edition. Preface to the First Edition.

Cytogenetics and Molecular Genetics: Sorting of Plant Chromosomes, J. Dolezel, M.A. Lysák, M. Kubaláková, H. Simková, J. Macas, and S. Lucretti. Quantitative DNA Fiber Mapping, H.-U.G. Weier. Primed in Situ Labeling, J. Hindkjaer, L. Bolund, and Steen Kolvraa. Measurements of Telomere Length on Individual Chromosomes by Image Cytometry, S.S.S. Poon and P.M. Lansdorp. Detection of Chromosome Translocation Products in Single Interphase Cell Nuclei, J. Fung, S. Munné, and H.-U.G. Weir.

Cell Function and Differentiation: Analysis of Mitochondria by Flow Cytometry, M. Poot and R.H. Pierce. Analysis of RNA Synthesis by Cytometry, P.O. Jensen, J. Larsen, and J.K. Larsen. Flow Cytometry of Erythropoiesis in Culture: Bivariate Profiles of Fetal and Adult Hemoglobin, R.M. Böhmer. Flow Cytometric Analysis of Human Hemopoietic Progenitor Differentiation by Assessing Cell Division Rate and Phenotypic Profile, L. Pierelli, G. Scambia, and A. Fattorossi.

Experimental Oncology: Cytometry of Antitumor Drug-Intracellular Target Interactions, P.J. Smith and M. Wiltshire. Monitoring of Cellular Resistance to Cancer Chemotherapy: Drug Retention and Efflux, A. Krishan. Resistance of Tumor Cells to Chemo- and Radiotherapy Modulated by the Three-Dimensional Architecture of Solid Tumors and Spheroids, R.E. Durand and P.L. Olive. Analysis of DNA Damage in Individual Cells, P.L. Olive, R.E. Durand, J.P. Banáth, and P.J. Johnston. Cytometric Methods to Analyze Ionizing-Radiation Effects, W.D. Wright, I. Lagroye, P. Zhang, R.S. Malyapa, and J.L. Roti Roti.

Clinical Oncology: Multiparameter Data Acquisition and Analysis of Leukocytes by Flow Cytometry, C.C. Stewart and S.J. Stewart. Immunophenotyping of Hemotological Malignancies by Laser Scanning Cytometry, R.J. Clatch. Immunophenotyping of Acute Leukemia: Utility of CD45 for Blast Cell Identification, J.-P. Vial and F. Lacombe. Cell Proliferation Markers in Human Solid Tumors: Assessing Their Impact in Clinical Oncology, M.G. Daidone, A. Costa, and R. Silvestrini. Detection of Minimal Residual Disease, A. Deptala and S.P. Mayer. Analysis of Human Tumors by Laser Scanning Cytometry, W. Corczyca, A. Deptala, E. Bedner, X. Li, M.R. Melamed, and Z. Darzynkiewicz. Laser Cytometry of Human Tissues and Tumors: Proliferation and Therapeutic Applications, D.A. Rew. Prediction and Precise Diagnosis of Diseases by Data Pattern Analysis in Multiparameter Flow Cytometry: Melanoma, Juvenile Asthma, and Human Immunodeficiency Virus Infection, G. Valet, H. Kahle, F. Otto, E. Bräutigam, and L. Kestens.

Microorganisms and Infectious Diseases: Flow Cytometric Analysis of Microorganisms, S.A. Sincock and J.P. Robinson. Staining and Measurement of DNA in Bacteria, H.B. Steen. Flow Cytometric Monitoring of Bacterial Susceptibility to Antibiotics, M. Walberg and H.B. Steen. Flow Cytometry for Evaluation and Investigation of Human Immunodeficiency Virus Infection, T.W. McCloskey. Index. Volumes in Series.


Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.

Key Features

@introbul:Key Features @bul: * Contains 56 extensive chapters authored by world authorities on cytometry

  • Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases
  • Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine
  • Complements the first and second editions on flow cytometry in the Methods in Cell Biology</> series and includes new sections on technology principles


Scientists, technicians and graduate students in basic research, industry, and clinical medicine.


No. of pages:
© Academic Press 2001
16th October 2000
Academic Press
Hardcover ISBN:
eBook ISBN:


Praise for the Second Edition "The second edition of Flow Cytometry provides a comprehensive and detailed foundation in the application of this diverse and rapidly expanding technology. The variety and scope of topics covered in this two-volume set are too large to cover in detail. The second edition of Flow Cytometry provides the investigator with a concise compilation of new and updated applications and procedures, updated references and a directory of the principal contributors to the field. The additions and improvements to the second edition are evidence of the impact this technology will continue to have on biological and medical sciences for years to come. --AMERICAN SCIENTIST

About the Serial Editors

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

About the Serial Volume Editors

Zbigniew Darzynkiewicz

Affiliations and Expertise


J. Paul Robinson

Affiliations and Expertise

Director of the Purdue University Cytometry Laboratories and Professor of Basic Medical Science and Biomedical Engineering, Purdue University, IN, USA.

Harry Crissman

Affiliations and Expertise

Cell and Molecular Biology Group, Los Alamos National Laboratory, New Mexico, U.S.A.