Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.

Key Features

@introbul:Key Features @bul: * Contains 56 extensive chapters authored by world authorities on cytometry * Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases * Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine * Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles


Scientists, technicians and graduate students in basic research, industry, and clinical medicine.

Table of Contents

Contents of Volume 63. Contributors. Preface to the Third Edition. Preface to the Second Edition. Preface to the First Edition. Cytogenetics and Molecular Genetics: Sorting of Plant Chromosomes, J. Dolezel, M.A. Lysák, M. Kubaláková, H. Simková, J. Macas, and S. Lucretti. Quantitative DNA Fiber Mapping, H.-U.G. Weier. Primed in Situ Labeling, J. Hindkjaer, L. Bolund, and Steen Kolvraa. Measurements of Telomere Length on Individual Chromosomes by Image Cytometry, S.S.S. Poon and P.M. Lansdorp. Detection of Chromosome Translocation Products in Single Interphase Cell Nuclei, J. Fung, S. Munné, and H.-U.G. Weir. Cell Function and Differentiation: Analysis of Mitochondria by Flow Cytometry, M. Poot and R.H. Pierce. Analysis of RNA Synthesis by Cytometry, P.O. Jensen, J. Larsen, and J.K. Larsen. Flow Cytometry of Erythropoiesis in Culture: Bivariate Profiles of Fetal and Adult Hemoglobin, R.M. Böhmer. Flow Cytometric Analysis of Human Hemopoietic Progenitor Differentiation by Assessing Cell Division Rate and Phenotypic Profile, L. Pierelli, G. Scambia, and A. Fattorossi. Experimental Oncology: Cytometry of Antitumor Drug-Intracellular Target Interactions, P.J. Smith and M. Wiltshire. Monitoring of Cellular Resistance to Cancer Chemotherapy: Drug Retention and Efflux, A. Krishan. Resistance of Tumor Cells to Chemo- and Radiotherapy Modulated by the Three-Dimensional Architecture of Solid Tumors and Spheroids, R.E. Durand and P.L. Olive. Analysis of DNA Damage in Individual Cells, P.L. Olive, R.E. Durand, J.P. Banát


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Praise for the Second Edition "The second edition of Flow Cytometry provides a comprehensive and detailed foundation in the application of this diverse and rapidly expanding technology. The variety and scope of topics covered in this two-volume set are too large to cover in detail. The second edition of Flow Cytometry provides the investigator with a concise compilation of new and updated applications and procedures, updated references and a directory of the principal contributors to the field. The additions and improvements to the second edition are evidence of the impact this technology will continue to have on biological and medical sciences for years to come. --AMERICAN SCIENTIST