Each chapter presents a detailed background of the described method, its theoretical foundations, and its applicability to different biomedical material. Updated chapters describe either the most popular methods or those processes that have evolved the most since the past edition. Additionally, a large portion of the volume is devoted to clinical cytometry. Particular attention is paid to applications of cytometry in oncology, the most rapidly growing area.

Key Features

@introbul:Key Features @bul: * Contains 56 extensive chapters authored by world authorities on cytometry * Covers a wide range of topics, including principles of cytometry and general methods, cell preparation, tandardization and quality assurance, cell proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, cell function and differentiation, experimental and clinical oncology, microorganisms, and infectious diseases * Describes in-depth the essential methods and scientific principles of flow and laser scanning cytometry and illustrates how they can be applied to the fields of biology and medicine * Complements the first and second editions on flow cytometry in the Methods in Cell Biology series and includes new sections on technology principles.


Scientists, technicians and graduate students in basic research, industry, and clinical medicine.

Table of Contents

Contents of Volume 64. Contributors. Preface to the Third Edition. Preface to the Second Edition. Preface to the First Edition. Principles of Cytometry and General Methods: A Brief History of Flow Cytometry and Sorting, M.R. Melamed. Principles of Flow Cytometry: An Overview, A.L. Givan. Laser Scanning Cytometry, L.A. Kamentsky. Principles of Confocal Microscopy, J.P. Robinson. Optical Measurements in Cytometry: Light Scattering, Extinction, Absorption, and Fluorescence, H.M. Shapiro. Flow Cytometric Fluorescence Lifetime Measurements, H.A. Crissman and J.A. Steinkamp. Principles of Data Acquisition and Display, H.M. Shapiro. Time as a Flow Cytometric Parameter, L. Seamer and L.A. Sklar. Protein Labeling with Fluorescent Probes, K.L. Holmes and L.M. Lantz. Cell Preparation: Preparation of Cells from Blood, J.P. McCoy, Jr.. Cell Preparation for the Identification of Leukocytes, C.C. Stewart and S.J. Stewart. Strategies for Cell Permeabilization and Fixation in Detecting Surface and Intracellular Antigens, S.K. Koestner and W.E. Bolton. Standardization, Quality Assurance: Stoichiometry of Immunocytochemical Staining Reactions, J.W. Jacobberger. Standardization and Quantitation in Flow Cytometry, R.A. Hoffman. Cell Proliferation: Methods to Identify Mitotic Cells by Flow Cytometry, G. Juan, F. Traganos, and Z. Darzynkiewicz. Cell Cycle Kinetics Estimated by Analysis of Bromodeoxyuridine Incorporation, N.H.A. Terry and R.A. White. Flow Cytomet


No. of pages:
© 2001
Academic Press
eBook ISBN:
Print ISBN:

About the serial-volume-editors

Zbigniew Darzynkiewicz

Affiliations and Expertise


J. Paul Robinson

Affiliations and Expertise

Director of the Purdue University Cytometry Laboratories and Professor of Basic Medical Science and Biomedical Engineering, Purdue University, IN, USA.

Harry Crissman

Affiliations and Expertise

Cell and Molecular Biology Group, Los Alamos National Laboratory, New Mexico, U.S.A.