Cytokinesis
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Cytokinesis, the latest volume in the Methods in Cell Biology series, looks at the latest advances in cytokinesis. Edited by leaders in the field, this volume presents proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies.
Key Features
- Covers sections on cytokinesis and emerging studies
- Presents chapters written by experts in the field
- Includes cutting-edge materials that supplement study
Readership
Researchers and students in cell, molecular and developmental biology
Table of Contents
- Series Editors
- Preface
- Chapter 1. Labeling membrane domains in dividing echinoderm eggs
- 1. General Care and Use of Sea Urchins
- 2. Plasma Membrane Labels
- 3. Intracellular Labeling
- Chapter 2. Dynamics of sphingomyelin- and cholesterol-enriched lipid domains during cytokinesis
- Introduction
- 1. Methods
- Conclusion
- Chapter 3. Analysis of protein dynamics during cytokinesis in budding yeast
- Introduction
- 1. Five-Dimensional Live-Cell Imaging
- 2. Photoactivation and Photoconversion
- 3. Fluorescence Recovery After Photobleaching
- Chapter 4. Imaging cytokinesis of Drosophila S2 cells
- Introduction
- 1. Methods
- Conclusion and Perspectives
- Chapter 5. Studying cytokinesis in Drosophila epithelial tissues
- Introduction
- 1. Methods and Experimental Setup
- 2. Data Processing and Analysis
- 3. Markers and Genetic Tools to Study Epithelial Cytokinesis In Vivo
- Conclusions and Future Perspectives
- Chapter 6. Visualization of cleavage furrow proteins in fixed dividing spermatocytes
- Introduction: Overview of Cytokinesis in Drosophila Spermatocytes
- 1. Fixation Protocols
- 2. Staining Protocols
- Chapter 7. Monitoring complete and incomplete abscission in the germ line stem cell lineage of Drosophila ovaries
- Introduction
- 1. Methods
- Conclusion
- Chapter 8. Incomplete cytokinesis/binucleation in mammals: The powerful system of hepatocytes
- Introduction
- 1. Experimental Procedures
- Conclusion
- Supplemendary Data
- Chapter 9. Specific membrane dynamics during neural stem cell division
- Introduction
- 1. General Considerations
- 2. Isolation and Detection of Released Postabscission Midbodies
- 3. Live-Cell Imaging
- 4. High-Voltage Electron Microscopy
- Summary
- Chapter 10. Understanding post-mitotic roles of the midbody during cell differentiation and polarization
- Introduction
- 1. Midbody and Epithelial Cell Polarity
- 2. Analysis of Midbody Uptake by Interphase Cells
- Summary
- Chapter 11. Micromanipulation of daughter cells for the study of cytokinetic abscission
- Introduction
- 1. Adhesive Micropatterns
- 2. Laser Ablation of the Intercellular Bridge
- Chapter 12. Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy
- Introduction
- 1. Methods
- Chapter 13. Analysis of cytokinesis by electron microscopy
- Introduction
- 1. Methods
- 2. Instrumentation and Materials
- 3. Discussion
- Chapter 14. Studying cytokinesis and midbody remnants using correlative light/scanning EM
- Introduction
- 1. Experimental Procedures
- Conclusion
- Chapter 15. Analysis of postcytokinetic roles of cytokinetic components in Caenorhabditis elegans
- Introduction
- 1. Generation of Transgenic Caenorhabditis elegans Expressing Fluorescent Cytokinetic Proteins
- 2. Creation of Conditional Mutations of Cytokinetic Genes in Caenorhabditis elegans Using the Somatic CRISPR-Cas9 Technique
- 3. Imaging of Cytokinesis and Postcytokinetic Events in Caenorhabditis elegans Larvae Using Spinning Disk Confocal Microscopy
- Conclusion
- Chapter 16. Variations on a theme: Imaging cytokinetic and stable rings in situ using Caenorhabditis elegans
- Introduction
- 1. Methods
- Conclusions
- Supplementary Data
- Chapter 17. Using fast-acting temperature-sensitive mutants to study cell division in Caenorhabditis elegans
- Introduction
- 1. Microscopy and Embryo Isolation
- 2. Using Fast-Acting Temperature Sensitive Mutant Embryos to Study Cytokinesis
- 3. Characterizing Temperature Sensitive Alleles
- 4. Choosing a Temperature Control Method
- Conclusions
- Chapter 18. Cytokinesis from nanometers to micrometers and microseconds to minutes
- Introduction
- 1. Micropipette Aspiration
- 2. Fluorescence Correlation Spectroscopy
- 3. Fluorescence cross-correlation spectroscopy
- 4. Fluorescence Recovery After Photobleaching
- Conclusion
- Chapter 19. An active contour ImageJ plugin to monitor daughter cell size in 3D during cytokinesis
- 1. Background
- 2. Methods
- Conclusion
- Appendix
- Chapter 20. Nuclear displacement and fluorescence recovery after photobleaching (FRAP) assays to study division site placement and cytokinesis in fission yeast
- Introduction
- 1. Methods
- Conclusion
- Chapter 21. Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers
- Introduction: The Bacterial Cytoskeleton
- 1. Protein Biochemistry: Expression, Purification, and Fluorescent Labeling
- 2. Fluorescence Imaging of Protein Dynamics
- 3. Data Analysis
- Conclusion
- Chapter 22. Preparation of centralspindlin as an active heterotetramer of kinesin and GTPase activating protein subunits for in vitro structural and functional assays
- Introduction
- 1. Purification of a Recombinant Caenorhabditis elegans Centralspindlin Complex Reconstituted in Bacterial Extract
- 2. Purification of Human Centralspindlin From HeLa Cells
- Conclusions
- Chapter 23. In vitro reactivation of the cytokinetic contractile ring of fission yeast cells
- Introduction
- 1. Methods
- Conclusion
- Chapter 24. Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis
- Introduction
- 1. Actin-Intact Extract Preparation
- 2. Fluorescent Probes
- 3. Microtubule Organizing Centers and Microtubule Nucleation
- 4. Preparation of Glass Surfaces for Imaging
- 5. Protein Immunodepletion
- 6. Supported Lipid Bilayers
- 7. Droplet Microfluidics
- Index
- Volumes in Series
Product details
- No. of pages: 480
- Language: English
- Copyright: © Academic Press 2017
- Published: January 2, 2017
- Imprint: Academic Press
- eBook ISBN: 9780128097922
- Hardcover ISBN: 9780128096734
About the Serial Volume Editor
Arnaud Echard
Arnaud Echard received a PhD in Molecular and Cell Biology from the University of Paris in 2000. In the lab of Dr. Bruno Goud at the Institut Curie, he discovered and characterized the first molecular motor of the kinesin superfamily localized at the Golgi apparatus. During his post-doctoral training at UCSF, USA in Pr. Pat O’Farrell Lab, he then worked on cell division in Drosophila embryos and carried out one of the first genome-wide RNAi-based screen designed to discovered conserved genes essential for cytokinesis.
In 2003, he has been hired at the French National Center for Scientific Research (CNRS) first at the Institut Curie, then at the Institut Pasteur. There, he set up the lab “Membrane Traffic and Cell Division” in the Cell Biology and Infection department in 2010.
Since 2006, he is part-time Professor of Molecular and Cellular Biology at the Ecole Polytechnique, France.
He has been awarded a Schlumberger Foundation Prize for Education and Research in 2010 and a Medical Research Foundation award in 2011. He has also been elected Vice-President (2012-2016) of the French Society for Cell Biology (SBCF) and President of the society since 2016, representing 180 labs and 1200 members.
Affiliations and Expertise
Institut Pasteur