Cytogenetics of Cells in Culture

Cytogenetics of Cells in Culture is a compendium of papers that deals with techniques to detect patterns of DNA replication, cellular control mechanisms, and chromosome analyses with automated instrumentation. Some papers discuss the effects of cell hybridization and of noxious substances such as radiation, chemical agents, and viruses on chromosomal patterns. Other papers focus on the influence of altered chromosomal complement on the survival of cells to long-term tissue culture or to ionizing radiation treatment. A couple of papers points out that naturally occurring chromosomal abnormalities in mammalian populations are relatively rare and are not necessarily associated with physical defects or disease. One paper notes that chromosomes in cells with different functions have different patterns of replication in which the changes are small and hidden. The chromosomes will usually be neutralized showing little of the characteristic pattern of replication or condensation during the early cleavage stages, that will later change into differentiated characteristic ways for each tissue type. Another paper describes chromosome breakage associated with viruses and DNA inhibitors. The compendium can prove beneficial for biochemists, micro-biologists, cellular researchers, and academicians involved in the study of cellular biology or physiology.

Dedication

List of Contributors

Preface

Cytogenetic Alterations in Mixed Cultures of Mammalian Somatic

Cells in Vitro

Hybridization of Somatic Cells in Vitro

Selection Pressure in Mammalian Cell Populations

Phase Cinematography Studies on the EfFects of Radiation and of Some Chemicals on Cells and Chromosomes

Cellular Interpretation of Aspects of the Acute Mammalian Radiation Syndrome

Retention of Euploidy and Mutagenicity of Heterochromatin in Culture

The Nature, Origin, and Genetic Implications of Structural Abnormalities of the Sex Chromosomes in Man

Chromosome Studies in the General Population

Alterations Chromosomiques dans des Cellules Cultivees en Presence d'Extraits Tissulaires Heterospecifiques

Chromosomal Studies on Human Leucocytes Following Treatment with Radioactive Iodine in Vivo and in Vitro

The Limited in Vitro Lifetime of Human Diploid Cell Strains

Regulation of DNA Replication and Variegation-Type Position Effects

Identification and Characterization of Human Chromosomes by DNA Replication Sequence

Mutagenic Response of Human Somatic Cell Lines

DNA Synthesis and Chromosomal Morphology of Chinese Hamster Cells Cultured in Media Containing N-Deacetyl-N-Methyl-colchicine (Colcemid)

Heterochromatin in the Human Male

Chromosome Breakage Associated with Viruses and DNA Inhibitors

Quantitation and Automation of Chromosomal Data with Special Reference to the Chromosomes of the Hamshire Pig (Sus Scrofa)

Author Index