Cytochrome P450, Volume 206

1st Edition

Protein-Dna Interactions

Serial Volume Editors: Michael Waterman Eric Johnson
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821074
eBook ISBN: 9780080883205
Imprint: Academic Press
Published Date: 20th November 1991
Page Count: 716


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Table of Contents

Site-Directed Mutagenesis, Protein Modification, and Structural Analysis:

D.W. Nebert and D.R. Nelson, P450 Gene Nomenclature Based on Evolution.

T.L. Poulos, Modeling of Mammalian P450s on Basis of P450cam X-Ray Structure.

S.G. Sligar, D. Filipovic, and P.S. Stayton, Mutagenesis of Cytochromes P450cam and b5.

F. Millett and L.M. Geren, Chemical Modification of Interaction between Adrenodoxin and Cytochrome P450scc.

L.S. Kaminsky, D. Dunbar, and W. Lawson, Azidowarfarin as Photoaffinity Probe of Cytochromes P450.

E. Szczesna-Skorupa and B. Kemper, Cell-Free Analysis of Targeting of Cytochrome P450 to Microsomal Membranes.

T. Omura and A. Oto, Biosynthesis and Intracellular Sorting of Mitochondrial Forms of Cytochrome P450.


F.J. Gonzalez, T. Aoyama, and H.V. Gelboin, Expression of Mammalian Cytochrome P450 Using Vaccinia Virus.

F.J. Gonzalez, S. Kimura, S. Tamura, and H.V. Gelboin, Expression of Mammalian Cytochrome P450 Using Baculovirus.

B.J. Clark and M.R. Waterman, Heterologous Expression of Mammalian P450 cDNA in COS Cells.

T.D. Porter and J.R. Larson, Expression of Mammalian P450s in Escherichia coli.

J. Doehmer and F. Oesch, V79 Chinese Hamster Cells Genetically Engineered for Stable Expression of Cytochromes P450.

C.L. Crespi, Expression of Cytochrome P450 cDNAs in Human B Lymphoblastoid Cells: Applications to Toxicology and Metabolite Analysis.

F.P. Guengerich, W.R. Brian, M.-A. Sari, and J.T. Ross, Expression of Mammalian~Cytochrome P450 Enzymes Using Yeast-Based Vectors.

Characterization of Genetic Polymorphism and Errors of Metabolism:<BR id="C


This volume represents the first collection of methodological approaches to P450 investigation which includes the use of molecular biological techniques. It is designed for use by those who study the P450 super family. Methods are presented for the identification and characterization of unique forms of P450 mRNA and protein, for expression of P450s in heterologous systems, for the study of human P450s, and for the characterization of P450s in different tissues.


Biochemists, cell biologists, molecular biologists, toxicologists, pharmacologists, microbiologists, immunologists, analytical chemists, biophysicists, endocrinologists, and graduate students in these disciplines.


No. of pages:
© Academic Press 1991
Academic Press
eBook ISBN:
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About the Serial Volume Editors

Michael Waterman Serial Volume Editor

Affiliations and Expertise

School of Medicine, Vanderbilt University, Nashville, Tennessee, U.S.A.

Eric Johnson Serial Volume Editor

Affiliations and Expertise

The Scripps Research Institute, La Jolla, California, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA