Description

This volume represents the first collection of methodological approaches to P450 investigation which includes the use of molecular biological techniques. It is designed for use by those who study the P450 super family. Methods are presented for the identification and characterization of unique forms of P450 mRNA and protein, for expression of P450s in heterologous systems, for the study of human P450s, and for the characterization of P450s in different tissues.

Readership

Biochemists, cell biologists, molecular biologists, toxicologists, pharmacologists, microbiologists, immunologists, analytical chemists, biophysicists, endocrinologists, and graduate students in these disciplines.

Table of Contents

Site-Directed Mutagenesis, Protein Modification, and Structural Analysis: D.W. Nebert and D.R. Nelson, P450 Gene Nomenclature Based on Evolution. T.L. Poulos, Modeling of Mammalian P450s on Basis of P450cam X-Ray Structure. S.G. Sligar, D. Filipovic, and P.S. Stayton, Mutagenesis of Cytochromes P450cam and b5. F. Millett and L.M. Geren, Chemical Modification of Interaction between Adrenodoxin and Cytochrome P450scc. L.S. Kaminsky, D. Dunbar, and W. Lawson, Azidowarfarin as Photoaffinity Probe of Cytochromes P450. E. Szczesna-Skorupa and B. Kemper, Cell-Free Analysis of Targeting of Cytochrome P450 to Microsomal Membranes. T. Omura and A. Oto, Biosynthesis and Intracellular Sorting of Mitochondrial Forms of Cytochrome P450. Heterologous~Expression: F.J. Gonzalez, T. Aoyama, and H.V. Gelboin, Expression of Mammalian Cytochrome P450 Using Vaccinia Virus. F.J. Gonzalez, S. Kimura, S. Tamura, and H.V. Gelboin, Expression of Mammalian Cytochrome P450 Using Baculovirus. B.J. Clark and M.R. Waterman, Heterologous Expression of Mammalian P450 cDNA in COS Cells. T.D. Porter and J.R. Larson, Expression of Mammalian P450s in Escherichia coli. J. Doehmer and F. Oesch, V79 Chinese Hamster Cells Genetically Engineered for Stable Expression of Cytochromes P450. C.L. Crespi, Expression of Cytochrome P450 cDNAs in Human B Lymphoblastoid Cells: Applications to Toxicology and Metabolite Analysis. F.P. Guengerich, W.R. Brian, M.-A. Sari, and J.T. Ross, Expression of Mammalian~Cytochrome P450 Enzymes Using Yeast-Based Vectors. Characterization of Genetic Polymorphism and Errors of Metabolism:

Details

No. of pages:
716
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
Print ISBN:
9780121821074
Electronic ISBN:
9780080883205

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY