The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Human Genomics and Genetics * Structure and Mechanism * Regulation of Expression * Metabolism * Invertibrate P450s


Bio and analytical chemists, micro, molecular, and cell biologists, pharmacologists, endocrinologists, and graduate students.

Table of Contents

Section I: Human Genomics and Genetics Mining Databases for Cytochrome P450 Genes. Sequence Alignments, Variabilities, and Vagaries. Human CYP Allele Database: Submission Criteria Procedures and Objectives. Fine-Scale Mapping of CYP Gene Clusters: An Example from Human CYP4 Family. Detection of Single Nucleotide Polymorphisms in CYP2B6 Gene. Genotyping Human Cytochrome P450 1B1 Variants. Genotyping Human CYP2A Variants. Section II: Structure and Mechanism Purification and Crystallization of N-Terminally Truncated Forms of Microsomal Cytochrome P450 2C5. Molecular Replacement in P450 Crystal Structure Determinations. Optical Biosensor and Scanning Probe Microscopy Studies of Cytochrome P450 Interactions with Redox Partners and Phospholipid Layers. Cryoradiolysis for the Study of P450 Reaction Intermediates. Analyzing Binding of N-Terminal Truncated, Microsomal Cytochrome P450s to Membranes. Sensitizer-Linked Substrates and Ligands: Ruthenium Probes of Cytochrome P450 Structure and Mechanism. Combining Pharmacophore and Protein Modeling to Predict CYP450 Inhibitors and Substrates. High Pressure: A New Tool to Study P450 Structure and Function. Section III: Regulation of Expression Use of in Vitro PXR Assays to Assess CYP3A4 Induction Potential of Drug Candidates. Analysis of CYP mRNA Expression by Branched DNA Technology. Ligand-Induced Coactivator Recruitment to PPAR—Characterized by Fluorescence Resonance Energy Transfer. Fluorescence-Based Ligand Binding Assays for PPARs. Developing Toxicologically Predictive Gene Sets Using cDNA Microarrays and Bayesian Classification. Direct Expression of


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© 2003
Academic Press
eBook ISBN:
Print ISBN:

About the editors

Eric Johnson

Affiliations and Expertise

The Scripps Research Institute, La Jolla, California, U.S.A.

Michael Waterman

Affiliations and Expertise

School of Medicine, Vanderbilt University, Nashville, Tennessee, U.S.A.


@from:PRAISE FOR THE SERIES @qu:"The Methods in Enzymology series represents the gold-standard." @source:—NEUROSCIENCE @qu:"Incomparably useful." @source:—ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:—BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:—CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:—AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." @source:—ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:—JOURNAL OF CHROMATOGRAPHY