Cytochrome P450, Part B, Volume 272

1st Edition

RNA Polymerase and Associated Factors, Part A

Serial Volume Editors: Eric Johnson Michael Waterman
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821739
eBook ISBN: 9780080883854
Imprint: Academic Press
Published Date: 3rd September 1996
Page Count: 468


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Table of Contents

Heterologous Expression of P450s: H.J. Barnes, Maximizing Expression of Eukaryotic Cytochrome P450s in Escherichia coli. C.W. Fisher, M.S. Shet, and R.W. Estabrook, Construction of Plasmids and Expression in Escherichia coli of Enzymatically Active Fusion Proteins Containing the Heme-Domain of a P450 Linked to NADPH-P450 Reductase. S.J. Pernecky and M.J. Coon, N-Terminal Modifications That Alter P450 Membrane Targeting and Function. F.P. Guengerich, M.V. Martin, Z. Guo, and Y.-J. Chun, Purification of Recombinant P450s from Bacteria. R.W. Estabrook, K.M. Faulkner, M.S. Shet, and C.W. Fisher, Application of Electrochemistry for P450-Catalyzed Reactions. D. Pompon, B. Louerat, A. Bronine, and P. Urban, Yeast Expression of Animal and Plant 450s in Optimized Redox Environments. U. Scheller, T. Juretzek, and W.-H. Schunck, Generation of the Cytosolic Domain of Microsomal P450 52A3 after High-Level Expression in Saccharomyces cerevisiae. S. Lecoeur, J.-C. Gautier, C. Belloc, A. Gauffre, and P. Beaune, Use of Heterologous Expression Systems to Study Autoimmune Drug-Induced Hepatitis. C.A. Lee, T.A. Kost, and C. Serabjit-Singh, Recombinant Baculovirus Strategy for Coexpression of Functional Human Cytochrome P450 and P450 Reductase. Enzyme Assays: H.K. Kroemer, S. Botsch, G. Heinkele, and M. Schick, In Vitro Assessment of Various Cytochromes P450 and Glucuronosyltransferases Using the Antiarrhythmic Propafenone as a Probe Drug. P.J. Wedlund and G.R. Wilkinson, In Vivo and in Vitro Measurement of CYP2C19 Activity. D. Lucas, J.-F. Menez, and F. Berthou, Chlorzoxazone: An in Vitro and in Vivo Substrate Probe for Liver CYP2E1. B.K.Tang and W. Kalow, Assays for CYP1A2 by Testing in Vivo Metabolism of Caffeine in Humans. D.J. Birkett, T. Andersson, and J.O. Miners, Assays of Omep


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Methods presented for

  • Heterologous expression of P450s
  • Enzyme assays
  • Determination of genotype
  • Insect P450s
  • Analysis of P450 structure
  • Regulation


Biochemists, toxicologists, pharmacologists, microbiologists, molecular biologists, cell biologists, analytical biochemists, endocrinologists, and graduate students in these disciplines.


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© Academic Press 1996
Academic Press
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About the Serial Volume Editors

Eric Johnson Serial Volume Editor

Affiliations and Expertise

The Scripps Research Institute, La Jolla, California, U.S.A.

Michael Waterman Serial Volume Editor

Affiliations and Expertise

School of Medicine, Vanderbilt University, Nashville, Tennessee, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA