Description

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Methods presented for * Heterologous expression of P450s * Enzyme assays * Determination of genotype * Insect P450s * Analysis of P450 structure * Regulation

Readership

Biochemists, toxicologists, pharmacologists, microbiologists, molecular biologists, cell biologists, analytical biochemists, endocrinologists, and graduate students in these disciplines.

Table of Contents

Heterologous Expression of P450s: H.J. Barnes, Maximizing Expression of Eukaryotic Cytochrome P450s in Escherichia coli. C.W. Fisher, M.S. Shet, and R.W. Estabrook, Construction of Plasmids and Expression in Escherichia coli of Enzymatically Active Fusion Proteins Containing the Heme-Domain of a P450 Linked to NADPH-P450 Reductase. S.J. Pernecky and M.J. Coon, N-Terminal Modifications That Alter P450 Membrane Targeting and Function. F.P. Guengerich, M.V. Martin, Z. Guo, and Y.-J. Chun, Purification of Recombinant P450s from Bacteria. R.W. Estabrook, K.M. Faulkner, M.S. Shet, and C.W. Fisher, Application of Electrochemistry for P450-Catalyzed Reactions. D. Pompon, B. Louerat, A. Bronine, and P. Urban, Yeast Expression of Animal and Plant 450s in Optimized Redox Environments. U. Scheller, T. Juretzek, and W.-H. Schunck, Generation of the Cytosolic Domain of Microsomal P450 52A3 after High-Level Expression in Saccharomyces cerevisiae. S. Lecoeur, J.-C. Gautier, C. Belloc, A. Gauffre, and P. Beaune, Use of Heterologous Expression Systems to Study Autoimmune Drug-Induced Hepatitis. C.A. Lee, T.A. Kost, and C. Serabjit-Singh, Recombinant Baculovirus Strategy for Coexpression of Functional Human Cytochrome P450 and P450 Reductase. Enzyme Assays: H.K. Kroemer, S. Botsch, G. Heinkele, and M. Schick, In Vitro Assessment of Various Cytochromes P450 and Glucuronosyltransferases Using the Antiarrhythmic Propafenone as a Probe Drug. P.J. Wedlund and G.R. Wilkinson, In Vivo and in Vitro Measurement of CYP2C19 Activity. D. Lucas, J.-F. Menez, and F. Berthou, Chlorzoxazone: An in Vitro and in Vivo Substrate Probe for Liver CYP2E1. B.K.Tang and W. Kalow, Assays for CYP1A2 by Testing in Vivo Metabolism of Caffeine in Humans. D.J. Birkett, T. Andersson, and J.O. Miners, Assays of Omep

Details

No. of pages:
468
Language:
English
Copyright:
© 1996
Published:
Imprint:
Academic Press
Print ISBN:
9780121821739
Electronic ISBN:
9780080883854

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY