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Current Topics in Cellular Regulation - 1st Edition - ISBN: 9780121528300, 9781483217291

Current Topics in Cellular Regulation, Volume 30

1st Edition

Editors: Bernard L. Horecker Earl R. Stadtman P. Boon Chock
eBook ISBN: 9781483217291
Imprint: Academic Press
Published Date: 28th November 1989
Page Count: 260
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Table of Contents

Eukaryotic DNA Methylation and Gene Expression

I. Introduction

II. In Vitro Methylation by Mammalian DNA Methyltransferases

III. Methylation of the Mouse ß-Globin (Major) Gene

IV. DNA Sequence of the Mouse ß-Globin (Major) Gene

V. Genomic Sequencing: Direct Sequencing of Native, Uncloned DNA

VI. Concluding Remarks


Regulation of Nitrogenase Activity by Reversible ADP Ribosylation

I. Properties of Nitrogenase

II. Observations Leading to the Discovery of Covalent Modification of Dinitrogenase Reductase

III. Identification of ADP-Ribose as the Modifying Group

IV. Purification and Properties of Dinitrogenase Reductase-Activating Glycohydrolase

V. Reaction Catalyzed by Dinitrogenase Reductase-Activating Glycohydrolase

VI. Discovery, Purification, and Properties of Dinitrogenase Reductase ADP-Ribosyltransferase

VII. Role of ADP Ribosylation in the Physiology of Rhodospirillum rubrum

VIII. Role of Nucleotides in the Regulation of Switch-Off

IX. Role of Pyridine Nucleotides in the Regulation of Switch-Off

X. Demonstration That ADP Ribosylation Is Reversible in Vivo

XI. Regulation of the ADP Ribosylation Cycle

XII. Regulation of Dinitrogenase Reductase in Dark-Grown Cells

XIII. Effect of the Oxidation State of Dinitrogenase Reductase on Regulation in Vivo

XIV. Role of Divalent Metals in Regulation in Vivo

XV. Is ADP Ribosylation of Dinitrogenase Reductase a Fine-Tuning Device or an On-Off Switch?

XVI. Relationship to the Glutamine Synthetase Cascade

XVII. Genes for Dinitrogenase Reductase-Activating Glycohydrolase and Dinitrogenase Reductase ADP-Ribosyltransferase

XVIII. Properties of the ADP-Ribosylated Dinitrogenase Reductase

XIX. ADP Ribosylation in Other Organisms

XX. Other Roles for Dinitrogenase Reductase-Activating Glycohydrolase and Dinitrogenase Reductase ADP-Ribosyltransferase

XXI. Concluding Remarks


Recognition of DNA by Type II Restriction Enzymes

I. Outline of Restriction/Modification Systems

II. Molecular Mechanism of DNA Recognition

III. Genetic Analysis of Restriction/Modification Systems

IV. Characterization of Proteins

V. Catalytic Mechanisms of Restriction Enzymes

VI. DNA Sequence Specificity

VII. Location of Recognition Sites on DNA

VIII. Future Prospects


The Intracellular Site of Action of Insulin: The Mitochondrial Krebs Cycle

I. Introduction

II. Anabolic Processes Stimulated by Insulin

III. Hexokinase and Mitochondria

IV. Action of Insulin on the Gluconeogenic and Oxidative Pathways

V. Effects of Insulin on Energy Production and Utilization

VI. Receptor and the Glucose Transporter in Relation to the Hexokinase Mitochondrial Theory of Insulin Action: Summary and Predictions


Nonequilibrium Isotope Exchange Methods for Investigating Enzyme Mechanisms

I. Introduction

II. Theory

III. Measurement of Flux Ratios

IV. Applications

V. Discussion


Transducin: A Signaling Switch Regulated by Guanine Nucleotides

I. Introduction

II. cGMP Cascade in Visual Excitation

III. Subunits of Transducin

IV. Functional Domains of Tα

V. Generalized Coupling Mechanism Among Transduction Enzymes

VI. Concluding Remarks


Coordinate Interactions of Cyclic Nucleotide and Phospholipid Metabolizing Pathways in Calcium-Dependent Cellular Processes

I. Introduction

II. Lipids and cAMP

III. Lipids and cGMP

IV. Cyclic Nucleotide-Dependent Protein Kinases and Lipids

V. Lipids and Phosphodiesterases

VI. Summary and Conclusions




Current Topics in Cellular Regulation: Volume 30 is a collection of papers that deals with eukaryotic DNA methylation, gene expression, regulation of nitrogenase activity, and the recognition of DNA by Type II restriction enzymes. Other papers discuss the mitochondrial Krebs cycle, enzyme mechanisms, transducin, and calcium-dependent cellular processes. One paper explains that methylation and demethylation of the DNA are complicated active processes, involving many aspects of the genome. The paper also notes that within the gross changes in methylation patterns, specific CpG sites play important roles in the expression or shutdown of target genes. Another paper concludes that, mono-ADP ribosylation being a mechanism of action of the diphtheria toxin, ADP ribosylation plays a role in normal cell metabolism by regulating the activity of key enzymes. One paper explains the significance of restriction enzymes into the nature of DNA-protein interactions; as well as the enzyme's possible role in discriminating between alternative DNA sequences. Biochemists, cellular researchers, micro-biologists, bio-physicists, and academicians involved in the study of cellular biology will find the collection very useful.


No. of pages:
© Academic Press 1989
28th November 1989
Academic Press
eBook ISBN:

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About the Editors

Bernard L. Horecker

Earl R. Stadtman

P. Boon Chock