Current Topics in Cellular Regulation - 1st Edition - ISBN: 9780121528218, 9781483217208

Current Topics in Cellular Regulation, Volume 21

1st Edition

Editors: Bernard L. Horecker Earl R. Stadtman
eBook ISBN: 9781483217208
Imprint: Academic Press
Published Date: 28th July 1982
Page Count: 328
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Table of Contents

List of Contributors

Roles of Eukaryotic Initiation Factor 2 Ancillary Factors in the Regulation of Eukaryotic Protein Synthesis Initiation

I. Introduction

II. eIF-2

III. Co-eIF-2A

IV. Co-eIF-2B

V. Co-eIF-2C

VI. eIF-2 Kinases


VIII. A Proposed Mechanism for Regulation of the Early Steps in Peptide Chain Initiation in Reticulocyte Lysates by eIF-2 Ancillary Factors

IX. Concluding Remarks


The Role of Phosphorylation in the Regulation of Eukaryotic Initiation Factor 2 Activity

I. Introduction

II. Translational Control in Rabbit Reticulocyte Lysate

III. Phosphorylation State of eIF-2

IV. Is Ternary Complex Formation the Only Initiation Step Affected in Hemin-Deficient Lysate?

V. Other Modifications of eIF-2 Which Provide Insights into the Regulation of Its Activity

VI. Role of Phosphorylation in the Regulation of eIF-2 Activity

VII. Summary


Probing the Function of the Eukaryotic 5′-Cap Structure Using Monoclonal Antibodies to Cap-Binding Proteins

I. Introduction

II. Monoclonal Antibodies to Cap-Binding Proteins

III. Reaction of Monoclonal Antibodies with High-Molecular-Weight Proteins Antigenically Related to the 24-Cap-Binding Protein

IV. ATP-Mg2+-Dependent Cross-Linking of Cap-Binding Proteins to mRNA

V. Effect of Anti-Cap-Binding Protein Monoclonal Antibodies on Protein Synthesis

VI. Cap-Binding Proteins and the Cytoskeleton

VII. Summary


Criteria for Establishment of the Biological Significance of Ribosomal Protein Phosphorylation

I. Introduction

II. Methodology

III. Changes in Vivo

IV. Signaling Systems

V. Reconstruction in Vitro


Casein Kinases—Multipotential Protein Kinases

I. Introduction

II. Physicochemical Characterization

III. Substrate Specificity

IV. Comparison with Casein Kinases from Mammary Gland

V. Regulation

VI. Phosphorylation of Endogenous Proteins

VII. Criteria for Classification of Casein Kinases

VIII. Conclusions


Phosphoprotein Phosphatases

I. Introduction

II. Multiple Forms and Substrate Specificity of Phosphoprotein Phosphatases

III. Purification and Properties of the Mr = 35,000 Phosphoprotein Phosphatases

IV. Relationship Between Phosphoprotein Phosphatases and Alkaline Phosphatases

V. Heat-Stable Protein Effectors

VI. High-Molecular-Weight Forms of Type I Phosphoprotein Phosphatase

VII. Regulation of Phosphoprotein Phosphatases

VIII. Conclusion


Note Added in Proof

ADP-Ribosylation Reactions

I. Mono(ADP-Ribosyl)ation and Poly(ADP-Ribosyl)ation

II. Poly(ADP-Ribosyl)ation Reactions in Vitro

III. Poly(ADP-Ribosyl)ation and DNA Repair

IV. Conclusions


Commitment to Terminal Differentiation and the Cell Cycle

I. Introduction

II. Induced Commitment to Terminal Erythroid Differentiation

III. Characteristics of the Commitment Process

IV. Inducer Effects in Relation to the Cell Division Cycle

V. Relation of the Cell Cycle to the Replication of α- and β-Globin DNA Sequences in Erythroleukemia Cells

VI. Summary


The Turnover Characteristics of Lactate Dehydrogenase

I. Introduction

II. Methodological Considerations

III. Isozyme Turnover

IV. Physiological Variations

V. Biodegradation and Its Regulation

VI. Concluding Comments


The Role of Urea Synthesis in the Removal of Metabolic Bicarbonate and the Regulation of Blood pH

I. Introduction

II. The Problem: Need for Disposal of HCO3-

III. The Solution: Acquisition of Proton from NH4+

IV. The Role of the Kidney

V. Regulation

VI. Discussion

VII. Summary

VIII. Appendix A: Equations for Relevant Catabolic Reactions or Processes

IX. Appendix B: Necessity for Disposal of HCO3-



Contents of Previous Volumes


Current Topics in Cellular Regulation, Volume 21 examines the advances in the general area of cellular regulation.

This book discusses the roles of eukaryotic initiation factor 2 ancillary factors in the regulation of eukaryotic protein synthesis initiation and phosphorylation state of eIF-2. The monoclonal antibodies to cap-binding proteins, criteria for establishment of the biological significance of ribosomal protein phosphorylation, and comparison with casein kinases from mammary gland are also elaborated. This text likewise covers the regulation of phosphoprotein phosphatases, poly(ADP-ribosyl)ation reactions in vitro, and induced commitment to terminal erythroid differentiation. Other topics include the turnover characteristics of lactate dehydrogenase and role of urea synthesis in the removal of metabolic bicarbonate and regulation of blood pH.

This volume is useful to biologists and researchers interested in basic mechanisms involved in the regulation of diverse cellular activities.


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© Academic Press 1982
Academic Press
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Ratings and Reviews

About the Editors

Bernard L. Horecker Editor

Earl R. Stadtman Editor