Current Biochemical Research in China

Contributors

Preface


1. Development of Biochemical Research in China

I. Introduction

II. General Situation, 1949-1966

III. Research Activities, 1949-1966

IV. 1966 and after

References


2. Chemical Synthesis and Structure-Function Studies of Bioactive Peptides

I. Improvements in Peptide Synthesis

II. Structure-Function Studies

References


3. Trichosanthin: From Chinese Herb Medicine to Ribosome Inactivating Protein

I. Introduction

II. Structure Investigation

III. Relationship of Structure and Function

IV. Prospects

References


4. Structure, Function, and Evolution of Certain Proteins

I. Introduction

II. Active Products of Porcine Trypsin after Autolysis

III. Comparative Study of Primary Structures of Lactate Dehydrogenase Isozymes M4 from Giant Panda and Other Related Mammals

IV. Perspective

References


5. Kinetics of Substrate Reaction during Modification of Enzyme Activity: Theory and Applications

I. Introduction

II. Kinetics of Substrate Reactions during Enzyme Modification

III. Experimental Studies

IV. Discussion

References


6. Mechanism of Snake Muscle Fructose 1,6-Bisphosphatase

I. Introduction

II. Phosphorylated Intermediate of Fructose 1,6-Bisphosphatase

III. Kinetics

IV. Regulation of Snake Muscle Fructose 1,6-Bisphosphatase

References


7. Urokinase and Its Related Proteases in Human Urine

I. Introduction

II. Benzamidine-Zinc Biaffinity Chromatography of Multiple Forms of Urokinase

III. Identification and Properties of Different Molecular Forms of Urokinase

IV. Prediction of Conformation of the Two Urokinase Fragments Involved in Inhibitor Binding

References


8. The Precise Structure of Insulin and Despentapeptide Insulin at High Resolution

I. Introduction

II. The Precise Structure of Insulin

III. Structure of Despentapeptide (B26-30) Insulin

IV. What Have We Learned from the Studies of Structure at High Resolution?

References


9. Correct Pairing of Insulin A and Β Chains in Solution and the Formation of the Native from the Scrambled Hormone

I. Introduction

II. Interaction of the Chains

III. Formation of Native Insulin by Protein Disulfide Isomerase

IV. Discussion

References


10. Modern Aspects of Insulin and C Peptide Synthesis

I. Introduction

II. Insulin A and Β Chains

III. Proinsulin C Peptide

References


11. Structure-Function Studies of Insulin

I. Semisynthetic Analogs

II. Analogs Prepared by Enzymatic Synthesis

III. Insulins from Different Species

IV. Analogs through Site-Directed Mutagenesis

References


12. Coupling Problems of Photophosphorylation

I. Coupling Mechanism of Photophosphorylation

II. The Stoichiometry between Phosphorylation and Electron Transport

III. The Degree of Coupling in Vivo and Its Regulation

References


13. Study on the Lipid-Protein Interactions in Η+-ATPase of Mitochondria

I. Lipid Structure Related to the Reactivation of Phospholipid-Deficient Η+ - ATPase

II. Mg2+-Mediated Change in the Physical State of Phospholipid Modulates Η+-ATPase Activity

References


14. A New Function of Proton Pumps in Membrane Fusion

I. Introduction

II. Proton Pumping Induces Membrane Fusion between Liposomes and Native Membranes Bearing Proton-Pumping Systems

III. Proton Pumping Induces Membrane Fusion of Reconstituted Cytochrome C Oxidase Phospholipid Vesicles with Liposomes

IV. Is Proton Pumping Induced Membrane Fusion an Energy-Dependent or H+-Consuming Process?

V. A New Hypothetical Model for Proton Pumping Induced Membrane Fusion

References


15. The Interaction of Anisodamine with Biological Membranes

I. Introduction

II. The Interaction of Anisodamine with Model Membranes

III. The Interaction of Anisodamine with Biological Membranes

IV. Effect of Anisodamine on the Phase Behavior of Phospholipid Liposomes

V. The Possible Mechanism of Action of Anisodamine on Biological Membranes

VI. Conclusions

References


16. Thirty Years of Study of Ribonucleic Acids

I. Work on tRNA before 1966

II. Total Synthesis of Yeast tRNAAla

III. Structure and Function Relationships of Yeast tRNAAla

IV. Other tRNA Research

V. RNA Sequencing

VI. 5 S rRNA Structure

VII. Molecular Evolution of Eukaryotic 5 S rRNAs

VIII. Biologically Active Oligonucleotides and Others

IX. Concluding Remarks

References


17. Cloning and Expression of Hepatitis B Virus Genes

I. Introduction

II. Cloning and Restriction Mapping of HBV Genomic DNA

III. Sequence Analysis of Cloned HBV Genomic DNA Subtype adr

IV. The Expression of the HBsAg Genes

V. Vaccinia Virus Vector System

VI. Expression of HBsAg with PreS—The Possible HBV Vaccine of a New Generation

VII. The Expression of the HBcAg Gene

VIII. Concluding Remarks

References


18. The Mechanism of the Replication of Cytoplasmic Polyhedrosis Virus of the Silkworm Bombyx mori

I. Introduction

II. Preparation of Cytoplasmic Polyhedrosis Virus

III. Relationship between the RNA Polymerase Activity and Infectivity

IV. Location and Replication of RNA Polymerase

V. Isolation and Reconstitution of RNA Polymerase and Methyltransferase

VI. Transcription and Translation in Vitro

VII. Conclusion

References


19. Molecular Aspects of Heat Shock Response in Human T Lymphocytes

I. The Heat Shock Response—A Mystery of Living Organisms

II. The Expression of HSP Genes in T Lymphocytes

III. The Effect of Heat Shock on T-Lymphocyte Activation

IV. The Transcription of Cellular Genes in Heat-Shocked T Lymphocytes

References


20. Phenotypes of Alpha-1-Proteinase Inhibitor and Their Racial Specificity and Geographical Variability in Mainland China

I. Introduction

II. Phenotypes and Allelic Frequencies of PI in Normal Chinese Populations

III. Is Variant Etokyo Pathogenic?

IV. The Genetic Types of Alpha-1-Proteinase Inhibitor in Chinese Patients with Pulmonary Emphysema

References


21. Studies of Platelet Membrane Glycoproteins Using Monoclonal Antibodies

I. Glycoprotein Ib-IX Complex and Platelet Adhesion

II. Glycoprotein IIb-IIIa Complex and Platelet Aggregation

III. Approach to Clinical Application

References

Index