Cryo-EM, Part C

Cryo-EM, Part C

Analyses, Interpretation, and Case Studies

1st Edition - September 30, 2010

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  • Editor: Grant Jensen
  • Hardcover ISBN: 9780123849939
  • eBook ISBN: 9780123849946

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Description

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Key Features

  • Includes time-tested core methods and new innovations applicable to any researcher
  • Methods included are useful to both established researchers and newcomers to the field
  • Relevant background and reference information given for procedures can be used as a guide

Readership

Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists

Table of Contents

  • 1. Analyses of Subnanometer Resolution Cryo-EM Density Maps
    Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio
    2. Methods for Segmentation and interpretation of cryo-EM data
    Niels Volkmann
    3. Integration of Cryo-EM with atomic and protein: protein interaction data
    Friedrich Forster
    4. Unified Data Resource for CryoEM
    Catherine L. Lawson
    5. Electron crystallography and aquaporins
    Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi
    6. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors
    Kenneth H. Downin and Eva Nogales
    7. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase
    Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward
    8. Multi-particle cryo-EM of ribosomes
    Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht
    9. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis
    Francisco Asturias, Edward J. Brignole
    10. Tomography of actin cytoskeletal networks
    Dorit Hanein
    11. Visual Proteomics
    Friedrich Förster, Bong-Gyoon Han, and Martin Beck
    12. Cryo-Electron Tomography of Eukaryotic Cells
    Ohad Medalia, Asaf Mader, Nadav Elad
    13. 3d Visualization Of Hiv Virions By Cryo Electron Tomography
    Jun Liu, Elizabeth R. Wright, Hanspeter Winkler
    14. Automation in Single-Particle Electron Microscopy: Connecting the Pieces
    Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter

Product details

  • No. of pages: 380
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: September 30, 2010
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123849939
  • eBook ISBN: 9780123849946

About the Serial Volume Editor

Grant Jensen

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