CRISPR-Cas Enzymes - 1st Edition - ISBN: 9780128167601

CRISPR-Cas Enzymes, Volume 616

1st Edition

Serial Volume Editors: Scott Bailey
Hardcover ISBN: 9780128167601
Imprint: Academic Press
Published Date: 1st February 2019
Page Count: 412
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Table of Contents

1. CRISPR bioinformatics
Rodolphe Barrangou
2. A method for one-step assembly of Class 2 CRISPR arrays
Chase Beisel
3. Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems
Ailong Ke
4. Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system
Ailong Ke
5. Site-specific fluorescent labeling of individual proteins within CRISPR complexes
Ilya J. Finkelstein
6. Fluorescence-based methods for measuring target interference by CRISPR-Cas systems
Dipali Sashital
7. Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry
Brian Bothner
8. ChIP-based assays to measure Cas protein binding to DNA in vivo
Joseph Wade
9. Live-cell super-resolution microscopy of Cascade-mediated DNA surveillance
Lennart Randau
10. In vitro assembly of Crispr-Cas Type III-A system
Eui-Jeon Woo
11. Cyclic oligoadenylate signalling by type III CRISPR systems
Malcolm White
12. A pipeline for characterization of Cas9 orthologues
Virginijus Siksnys
13. Preparation and use of Cas12a RNPs for genome editing experiments
Martin Jinek
14. Discovery and Mechanistic Studies of A Thermophilic Cas9 towards Gene Editing and Cancer Diagnostics Applications
Hong Li
15. Kinetic characterization of Cas9 enzymes
David Taylor
16. Single-molecule FRET studies of Cas9 endonuclease
Chirlmin Joo
17. CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA
Konstantin Severinov
18. dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST)
Erik Sontheimer
19. CRISPR-Cas10 assisted editing of virulent bacteriophages
Asma Hatoum-Aslan
20. Reconstituted in vitro CRISPR adaptation and its detection by PCR
Peter Fineran
21. Quantitative deep sequencing of CRISPR-Cas spacer acquisition
Peter Fineran


Description

CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on the CRISPR-Cas Enzymes

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
412
Language:
English
Copyright:
© Academic Press 2019
Published:
Imprint:
Academic Press
Hardcover ISBN:
9780128167601

Reviews

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY
"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Ratings and Reviews


About the Serial Volume Editors

Scott Bailey Serial Volume Editor

Scott Bailey is at Johns Hopkins Bloomberg School of Public Health, USA

Affiliations and Expertise

Johns Hopkins Bloomberg School of Public Health, USA