CRISPR-Cas Enzymes - 1st Edition - ISBN: 9780128167601

CRISPR-Cas Enzymes, Volume 616

1st Edition

Serial Volume Editors: Scott Bailey
Hardcover ISBN: 9780128167601
Imprint: Academic Press
Published Date: 8th February 2019
Page Count: 452
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Table of Contents

    1. Predicting and visualizing features of CRISPR–Cas systems
    2. Matthew A. Nethery and Rodolphe Barrangou

    3. Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR–Cas systems
    4. Yibei Xiao and Ailong Ke

    5. Sortase-mediated fluorescent labeling of CRISPR complexes
    6. Kaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein

    7. Fluorescence-based methods for measuring target interference by CRISPR–Cas systems
    8. Phong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital

    9. Probing Cascade complex composition and stability using native mass spectrometry techniques
    10. Angela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner

    11. High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP
    12. Joseph T. Wade

    13. Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance
    14. Bartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau

    15. In vitro assembly of thermostable Csm complex in CRISPR–Cas type III/A system
    16. Kwang-Hyun Park, Yan An and Eui-Jeon Woo

    17. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems
    18. Christophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White

    19. A pipeline for characterization of novel Cas9 orthologs
    20. Tautvydas Karvelis, Joshua K. Young and Virginijus Siksnys

    21. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells
    22. Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek

    23. Directed evolution studies of a thermophilic Type II-C Cas9
    24. Travis H. Hand, Anuska Das and Hong Li

    25. Kinetic characterization of Cas9 enzymes
    26. Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W. Taylor and Kenneth A. Johnson

    27. Single-molecule FRET studies of Cas9 endonuclease
    28. Viktorija Globyte and Chirlmin Joo

    29. CRISPR–Cas molecular beacons as tool for studies of assembly of CRISPR–Cas effector complexes and their interactions with DNA
    30. Vladimir Mekler, Konstantin Kuznedelov, Leonid Minakhin, Karthik Murugan, Dipali G. Sashital and Konstantin Severinov

    31. Adapting dCas9-APEX2 for subnuclear proteomic profiling
    32. Xin D. Gao, Tomás C. Rodríguez and Erik J. Sontheimer

    33. CRISPR–Cas10 assisted editing of virulent staphylococcal phages
    34. S.M. Nayeemul Bari and Asma Hatoum-Aslan

    35. Reconstitution of CRISPR adaptation in vitro and its detection by PCR

Robert D. Fagerlund, Timothy J. Ferguson, Howard W.R. Maxwell, Helen K. Opel-Reading, Kurt L. Krause and Peter C. Fineran


CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on the CRISPR-Cas Enzymes


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


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© Academic Press 2019
Academic Press
Hardcover ISBN:


Praise for the Series:
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"The work most often consulted in the lab." --ENZYMOLOGIA
"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

Ratings and Reviews

About the Serial Volume Editors

Scott Bailey Serial Volume Editor

Scott Bailey is at Johns Hopkins Bloomberg School of Public Health, USA

Affiliations and Expertise

Johns Hopkins Bloomberg School of Public Health, USA