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CRISPR and RNAi Systems - 1st Edition - ISBN: 9780128219102

CRISPR and RNAi Systems

1st Edition

Nanobiotechnology Approaches to Plant Breeding and Protection

Editors: Kamel Abd-Elsalam Ki-Taek Lim
Paperback ISBN: 9780128219102
Imprint: Elsevier
Published Date: 1st March 2021
Page Count: 702
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Table of Contents

1. CRISPR and RNAi: A Promising Tool in Plant Nanobiotechnology
2. RNAi/CRISPR based system: Targeted genome engineering for insect control
3. CRISPR/Cas9 regulations in plant science
4. Are CRISPR/Cas9 and RNAi-based new technologies to relocate crop pesticides?
5. CRISPR-Cas epigenome editing technology: improving crop resistance to pathogens
6. CRISPR System for the development of disease-resistance in horticulture crops
7. CRISPR and RNAi technology for crop improvements in the developing countries
8. CRISPR/Cas9 applications for virus resistance
9. Current Trends and Recent Progress of Genetic Engineering in Genus Phytophthora using CRISPR Systems
10. CRISPR/Cas 9 and Cas13a Systems: a promising tool for plant breeding
11. CRISPR/Cas techniques: a new method for RNA interference in cereals
12. Different ways of wheat genetic transformation for efficient genome editing
13. CRISPR-Cas for genome editing in Cotton
14. Wheat Starch via CRISPR System: Biosynthesis and Regulatory Pathways
15. Role of CRISPR/Cas system in altering phenolics and carotenoids biosynthesis for plants defense
16. Fungal Genome Editing Using CRISPR-Cas Nucleases: A New Tool for the Management of Plant Diseases
17. CRISPR-Cas systems as antimicrobial agents for agri-food pathogens.
18. CRISPR Interference System: A potential strategy to inhibit pathogenic biofilm in agri-food sector
19. Patenting Dynamics in CRISPR gene editing technologies
20. Databases and Bioinformatics Tools for Genome Editing in Plants using CRISPR/Cas and RNA interference
21. RNAi and CRISPR/Cas9 techniques for controlling mycotoxins
22. Role of Small RNA and RNAi Technology Towards Improvement of Abiotic Stress Tolerance in Plants
23. RNAi based system a new tool for insects’ control
24. RNAi strategy for management of phytopathogenic fungi
25. CRISPR applications in plant bacteriology: today and future perspectives
26. RNAi-based gene silencing in plant parasitic nematodes: A road towards crop improvement
27. RNAi mediated viral disease resistance in crop plants
28. Phytoalexin biosynthesis through RNAi for disease resistance
29. Polymer and lipid-based nanoparticles to deliver RNAI and CRISPR systems
30. Inorganic Smart Nanoparticles: New Tool to Deliver CRISPR systems into Plant Cells
31. Regulatory aspects, risk assessment, and toxicity associated with RNAi and CRISPR methods
32. Gene editing in oomycetes and filamentous fungi using CRISPR-Cas technology
33. CRISPR System to enhance abiotic stress tolerance in plants


CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects and various plant pathogens. This book is an important reference source for both plant scientists and environmental scientists who want to understand how nanobiotechnologically-based approaches are being used to create more efficient plant protection and plant breeding systems.

Plants are vulnerable to pathogens including fungi, bacteria and viruses which can cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture.

Key Features

  • Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection
  • Outlines the major techniques and applications of both CRISPR and RNAi technologies
  • Assesses the major challenges of escalating these technologies on a mass scale


Plant scientists and environmental scientists


No. of pages:
© Elsevier 2021
1st March 2021
Paperback ISBN:

Ratings and Reviews

About the Editors

Kamel Abd-Elsalam

Dr. Kamel A. Abd-Elsalam is currently the head researcher at Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. Dr. Kamel’s research interests include developing, improving and deploying plant biosecurity diagnostic tools, understanding and exploiting fungal pathogen genomes and developing eco-friendly hybrid nanomaterials for controlling toxicogenic fungi and plant diseases. He has published 8 book chapters, 6 review articles, 1 translated book, and more than 120 research articles in international peer reviewed journals including Fungal Diversity, Fungal Biology, FEMS Review Microbiology, PLOS One and PLOS Genetics. He is associate editor for Mycosphere, and review editor for Frontiers in Genomic Assay Technology and served as a reviewer for a number of journals, including IET Nanotechnology, Fungal Diversity, BMC Genomics, Foodborne Pathogens and Diseases. Dr. Kamel has also served as molecular mycologist for 5 years in the Department Botany and Microbiology Department, College of Science, King Saud University, Saudi Arabia. He received the Federation of Arab Scientific Research Councils Prize for distinguished scientific research in biotechnology (fungal genomics) during 2014 (first ranking). Dr. Kamel has pursued his Ph.D. in Molecular Plant Pathology from Christian Alberchts University of Kiel (Germany) and Suez Canal University (Egypt) and then been awarded postdoctoral fellowship from Christian Alberchts University of Kiel in 2008. Dr. Kamel served as visiting associate professor in Institute of Excellence in Fungal Research, Mae Fah Luang University, Thailand, Institute of Microbiology, TUM, Germany, Laboratory of Phytopathology, Wageningen University, The Netherlands and Plant Protection Department, Sassari University, Italy. Kamel Abd-Elsalem is Research Associate Professor at the Agricultural Research Center, Plant Pathology Research Institute, Egypt. His research focuses in the areas of nanomaterials for plant sciences, environmental sciences and agriculture.

Affiliations and Expertise

Research Associate Professor, Agricultural Research Center, Plant Pathology Research Institute, Cairo, Egypt

Ki-Taek Lim

Ki-Taek Lim is Assistant Professor, Department of Biosystems Engineering, Kangwon National University, South Korea. His research focuses on biosystems engineering.

Affiliations and Expertise

Assistant Professor, Department of Biosystems Engineering, Kangwon National University, Gangwon-do, South Korea