Correlative Microscopy In Biology

Preface


1 Biological Specimen Preparation for Correlative Light and Electron Microscopy

Introduction

Methods

Applications of Correlative Light and Electron Microscopy

References


2 Light and Scanning Electron Microscopy in a Combined Instrument

Introduction

Description of the Combined Instrument

Preparation and Staining Method for LM/SEM Specimens

Quantitative Measurements in the LM/SEM

Cathodoluminescence in the LM/SEM

Biomedical Applications

Developments with Another Type of Scanning Electron Microscope

Conclusions

References


3 Confocal Microscopy in Comparison with Electron and Conventional Light Microscopy

Introduction

The CSLM Instrument

The Morphology of the Nucleoid of Escherichia coli

Characterization of Cell Shape


3-D Imaging Techniques with CSLM

Conclusion and Outlook

References


4 Combined Light and Electron Microscopy of Central Nervous System Neurons and Their Afferent and Efferent Synaptic Connections

Introduction

Morphological Techniques: Correlative Light and Electron Microscopy of Golgi-Impregnated Neurons

Immunocytochemical Techniques: Correlative Light and Electron

Microscopy of Neurons Labeled Immunocytochemically for the

Presence of Neuroactive Substances of Their Synthesizing Enzymes

Correlative Light and Electron Microscopy of Neurons Labeled with PHA-L

References


5 Intracellular Staining of Neurons in the Central Nervous System: Combined Physiological, Morphological, and Cytological Analysis of Identified Neurons

Introduction

Methods

Tissue Processing

Data Analysis

Limitations of the Method

Conclusions

References


6 Correlative Microscopy of Catecholamines and Neuropeptides in Adrenal Medulla

Introduction

Vibratome Sectioning and Fluorescence Microscopy

Immunocytochemistry

Possible Modifications

Double-Labeling Methods for Studying Coexistence

Advantages and Limitations of Combined Faglu-Fluorescence and Immunocytochemistry Method

References


7 Sequential Immunocytochemical and Silver Staining of Neuroendocrine Cells in the Same Section

Introduction

Description of Sequential Staining Technique

Comments on Methodology

Applications

References


8 Correlative Light and Electron Microscopic Method for the Visualization of the Same in Vitro Cell Using Radioautography and Serial Sectioning

Introduction

Methodology

Applications

References


9 Correlative Light and Transmission Electron Microscopy of Mitotic Cells in the Nervous System

Introduction

Mitotic Cells in the Embryonic CNS

Mitotic Cells in the CNS during Myelination

Identification of Mitotic Cells in the Mature and Aged CNS

Applications

References


10 Light and Scanning Electron Microscopy of the Same Metaphase Chromosomes

Introduction

Methodology

Applications of Correlative Light Microscopy and SEM

References


11 Correlative Light and Electron Microscopy on the Same Epoxy Section

Introduction

Methodology

Applications

References


12 Correlative Microscopy of Skeletal Muscle and Endometrium

Introduction

Materials and Methods for Skeletal Muscle

Materials and Methods for Endometrium

Discussion of Methodology and Selected Results

References


13 Correlative Microscopy of Isolated Glomeruli

Introduction

Method for Correlative Microscopy

Illustrated Examples

Concluding Remarks

References


14 Correlative Microscopy of Cell Surface and Underlying Structures

Introduction

Techniques Using Light Microscopy for Cytological Features in Combination with Scanning Electron Microscopy for Surface Structures

Techniques Using Backscattered and Secondary Electron Imaging on Cells and Tissues Processed for Scanning Electron Microscopy

Applications and Future Prospects

References


15 Correlative Light, Scanning, and Transmission Electron Microscopy of Deembedded Tissues

Introduction

Methodology

Applications

References


16 Immunoelectron Microscopic Mirror Method: A Correlative Study

Introduction

Methodology

Correlative Light and Electron Microscopy

Discussion

References


17 The Detection of Amyloid Using Correlative Polarizing Light Microscopy and Electron Microscopy

Introduction

Amyloidosis

Methods

Concluding Remarks

References


18 Correlative Microscopy of Human Vitreous Body

Introduction

Method

Conclusion

References


19 A New Method for Bacterial Identification by Studying the Same Section with Light Microscopy and Scanning and Transmission Electron Microscopy

Introduction

Light Microscopy, Scanning and Transmission Electron Microscopy as Separated Single Tool for Detecting Bacteria within Gingival Tissues

Method

Application

References


20 Correlative Light and Scanning Electron Microscopy of Thick Sections and Isolated Cells

Introduction

Operating Principles

Application of Correlative Scanning Electron Microscopy

Stepwise Procedure

Conclusion

References

Index