Correlative Microscopy In Biology - 1st Edition - ISBN: 9780123339225, 9780323149860

Correlative Microscopy In Biology

1st Edition

Instrumentation and Methods

Editors: M.A. Hayat
eBook ISBN: 9780323149860
Imprint: Academic Press
Published Date: 28th September 1987
Page Count: 451
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Description

Correlative Microscopy in Biology: Instrumentation and Methods presents the detailed methodology of biological correlative microscopy, a technology that allows the acquisition of multiple data from single tissue block, cell, or section. The chapters in the book include detailed and complete instructions on the preparatory procedures.
The book has 20 chapters that deal with various forms and systems of microscopy. Some of the forms and methods used in the book include light, scanning electron, fluorescence, scanning transmission electron, and ion microscopy, as well as combined light and electron and transmission electron microscope. Other methods and their applications are all discussed in detail in the book.
This book will help students apply the methods without outside help as each methodology is presented in a step-by-step approach, including applications and techniques. Aside from students, the book will also be good reference for teachers, scientists, and researchers in the fields of biology, biochemistry, and medicine.

Table of Contents


Preface

1 Biological Specimen Preparation for Correlative Light and Electron Microscopy

Introduction

Methods

Applications of Correlative Light and Electron Microscopy

References

2 Light and Scanning Electron Microscopy in a Combined Instrument

Introduction

Description of the Combined Instrument

Preparation and Staining Method for LM/SEM Specimens

Quantitative Measurements in the LM/SEM

Cathodoluminescence in the LM/SEM

Biomedical Applications

Developments with Another Type of Scanning Electron Microscope

Conclusions

References

3 Confocal Microscopy in Comparison with Electron and Conventional Light Microscopy

Introduction

The CSLM Instrument

The Morphology of the Nucleoid of Escherichia coli

Characterization of Cell Shape

3-D Imaging Techniques with CSLM

Conclusion and Outlook

References

4 Combined Light and Electron Microscopy of Central Nervous System Neurons and Their Afferent and Efferent Synaptic Connections

Introduction

Morphological Techniques: Correlative Light and Electron Microscopy of Golgi-Impregnated Neurons

Immunocytochemical Techniques: Correlative Light and Electron

Microscopy of Neurons Labeled Immunocytochemically for the

Presence of Neuroactive Substances of Their Synthesizing Enzymes

Correlative Light and Electron Microscopy of Neurons Labeled with PHA-L

References

5 Intracellular Staining of Neurons in the Central Nervous System: Combined Physiological, Morphological, and Cytological Analysis of Identified Neurons

Introduction

Methods

Tissue Processing

Data Analysis

Limitations of the Method

Conclusions

References

6 Correlative Microscopy of Catecholamines and Neuropeptides in Adrenal Medulla

Introduction

Vibratome Sectioning and Fluorescence Microscopy

Immunocytochemistry

Possible Modifications

Double-Labeling Methods for Studying Coexistence

Advantages and Limitations of Combined Faglu-Fluorescence and Immunocytochemistry Method

References

7 Sequential Immunocytochemical and Silver Staining of Neuroendocrine Cells in the Same Section

Introduction

Description of Sequential Staining Technique

Comments on Methodology

Applications

References

8 Correlative Light and Electron Microscopic Method for the Visualization of the Same in Vitro Cell Using Radioautography and Serial Sectioning

Introduction

Methodology

Applications

References

9 Correlative Light and Transmission Electron Microscopy of Mitotic Cells in the Nervous System

Introduction

Mitotic Cells in the Embryonic CNS

Mitotic Cells in the CNS during Myelination

Identification of Mitotic Cells in the Mature and Aged CNS

Applications

References

10 Light and Scanning Electron Microscopy of the Same Metaphase Chromosomes

Introduction

Methodology

Applications of Correlative Light Microscopy and SEM

References

11 Correlative Light and Electron Microscopy on the Same Epoxy Section

Introduction

Methodology

Applications

References

12 Correlative Microscopy of Skeletal Muscle and Endometrium

Introduction

Materials and Methods for Skeletal Muscle

Materials and Methods for Endometrium

Discussion of Methodology and Selected Results

References

13 Correlative Microscopy of Isolated Glomeruli

Introduction

Method for Correlative Microscopy

Illustrated Examples

Concluding Remarks

References

14 Correlative Microscopy of Cell Surface and Underlying Structures

Introduction

Techniques Using Light Microscopy for Cytological Features in Combination with Scanning Electron Microscopy for Surface Structures

Techniques Using Backscattered and Secondary Electron Imaging on Cells and Tissues Processed for Scanning Electron Microscopy

Applications and Future Prospects

References

15 Correlative Light, Scanning, and Transmission Electron Microscopy of Deembedded Tissues

Introduction

Methodology

Applications

References

16 Immunoelectron Microscopic Mirror Method: A Correlative Study

Introduction

Methodology

Correlative Light and Electron Microscopy

Discussion

References

17 The Detection of Amyloid Using Correlative Polarizing Light Microscopy and Electron Microscopy

Introduction

Amyloidosis

Methods

Concluding Remarks

References

18 Correlative Microscopy of Human Vitreous Body

Introduction

Method

Conclusion

References

19 A New Method for Bacterial Identification by Studying the Same Section with Light Microscopy and Scanning and Transmission Electron Microscopy

Introduction

Light Microscopy, Scanning and Transmission Electron Microscopy as Separated Single Tool for Detecting Bacteria within Gingival Tissues

Method

Application

References

20 Correlative Light and Scanning Electron Microscopy of Thick Sections and Isolated Cells

Introduction

Operating Principles

Application of Correlative Scanning Electron Microscopy

Stepwise Procedure

Conclusion

References

Index






Details

No. of pages:
451
Language:
English
Copyright:
© Academic Press 1987
Published:
Imprint:
Academic Press
eBook ISBN:
9780323149860

About the Editor

M.A. Hayat

Dr. Hayat serves as Distinguished Professor in the Department of Biological Sciences at Kean University. He is an internationally renowned scholar in the fields of electron microscopy and cancer research, has authored and edited more than 31 books, several of these recently with Elsevier (dealing with immunohistochemistry of carcinomas, cancer imaging, and autophagy).

Dr. Hayat has published chapters in 54 books, and authored 10 of them. Seven major publishers have published his books: Cambridge University Press, Elsevier, Academic Press, McGraw-Hill, Plenum Press, CRC Press, and Van Nostrand Reinhold Company. Dr. Hayat’s books have been published in Japanese. He has also published a large number of articles in scientific journals and established the Electron Microscopy Center at Kean University.

Degree Information: Ph.D., Biology, Indiana University

Courses Taught: Electron Microscopy; Cell Biology; Seminar

Primary Area of Expertise: Electron microscopy, cell biology, cancer, health and disease. His research interests include immunohistochemistry and in situ hybridization of human carcinomas, including brain metastases and various primary cancers (lung, breast, prostate, colorectal, pancreatic, ovarian, gastrointestinal, and liver).

Advice For Students Preparing for Your Class: "Read the books and work very hard."

Affiliations and Expertise

Department of Biological Science, Kean University, USA

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