Correlative Light and Electron Microscopy III - 1st Edition - ISBN: 9780128099759, 9780128099766

Correlative Light and Electron Microscopy III, Volume 140

1st Edition

Serial Editors: Thomas Muller-Reichert Paul Verkade
eBook ISBN: 9780128099766
Hardcover ISBN: 9780128099759
Imprint: Academic Press
Published Date: 23rd May 2017
Page Count: 370
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Table of Contents

1. Millisecond time-resolved CLEM

  Gaia Pigino

2. Super resolution LM und SEM of high-pressure frozen C. elegans

  Christian Stigloher

3. Preservation fluorescence, super res CLEM

  Errin Johnson

4. Targeted ultramicrotomy

  Yannick Schwab

5. CEMOVIS-CLEM

  Yannick Schwab

6. APEX in Tissue

  Robert G. Parton

7. Corrsight mit IBIDI flowthrough chamber

  Miriam Susanna Lucas

8. Corrsight

  Claudia S. Lopez

9. Correlative Light Atomic Force Electronic Microscopy (CLAFEM)

  Frank Lafont

10. Atmospheric EM CLEM

  Chikira Sato

11. Correlated Fluo Light SEM

  Filip Braet

12. Near infrared branding + SBF SEM

  Paul Verkade

13. 4. Correlative MicroCT

  Matthia Andrea Karreman

14. High-precision correlation

  Wanda Kukulski

15. CLEM acquisition

  Martin Schorb and Frank Sieckmann

16. ecCLEM mapping software

  Xavier Heiligenstein and Perrine Paul-Gilloteaux


Description

Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics.

Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.

Key Features

  • Contains contributions from experts in the field
  • Covered topics include targeted ultramicrotomy and high-precision correlation
  • Presents recent advances and currently applied correlative approaches
  • Gives detailed protocols allowing the application of workflows in one’s own laboratory setting
  • Covers CLEM approaches in the context of specific applications
  • Aims to stimulate the use of new combinations of imaging modalities

Readership

Researchers and students in cell, molecular and developmental biology


Details

No. of pages:
370
Copyright:
© Academic Press 2017
Published:
Imprint:
Academic Press
eBook ISBN:
9780128099766
Hardcover ISBN:
9780128099759

About the Serial Editors

Thomas Muller-Reichert Serial Editor

Dr. Thomas Müller-Reichert is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in meiosis, mitosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in the early embryo of the nematode Caenorhabditis elegans and in tissue culture cells. He got his PhD degree from the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards to the EMBL in Heidelberg (Germany) for a post-doc with Dr. Tony Hyman. He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA) and set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is head of the Core Facility Cellular Imaging (CFCI) of the Medical Faculty Carl Gustav Carus of the TU Dresden (Germany).

Together with Dr. Paul Verkade he has developed a rapid transfer system for high-pressure freezing used for Correlative Light and Electron Microcopy. He has organized a number microscopy conferences and taught in several (CL)EM courses. He edited an MCB volume on the Electron Microscopy of Model Systems.

Affiliations and Expertise

Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, Dresden, Germany

Paul Verkade Serial Editor

Dr. Paul Verkade’s research focuses on the sorting mechanisms in intracellular transport pathways. His main tools are microscopy techniques, with an emphasis on electron microscopy (EM) in which field he has published over 50 papers. He has studied and got his PhD degree at the University of Utrecht, the Netherlands. After his post-doc time at the EMBL, Heidelberg, Germany in the group of Kai Simons and setting up a new EM lab at the Max Planck Institute for Molecular Cell Biology in Dresden, Germany he moved to the University of Bristol, UK in 2006. Here he set up a new EM unit as part of the Wolfson Bioimaging Facility, a fully integrated light and electron microscopy centre.

To support his transport studies, part of his research is to develop techniques and tools for the use of Correlative Light Electron Microscopy (CLEM). Amongst other things he has developed the Rapid Transfer System for the EMPACT2 high-pressure freezer together with Leica Microsystems. This allows for the combination of time-resolved CLEM with optimal preservation of ultrastructure for EM.

Dr. Verkade is chair of the Electron Microscopy section of the Royal Microscopical Society (RMS) and of the Cryo Microscopy Group, affiliated to the RMS. He has organised and taught on a large number of courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. He is also the principle organiser of the EMBO practical course on CLEM.

Affiliations and Expertise

Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology & Pharmacology, University of Bristol, Bristol, UK