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1. Millisecond time-resolved CLEM
2. Super resolution LM und SEM of high-pressure frozen C. elegans
3. Preservation fluorescence, super res CLEM
4. Targeted ultramicrotomy
6. APEX in Tissue
Robert G. Parton
7. Corrsight mit IBIDI flowthrough chamber
Miriam Susanna Lucas
Claudia S. Lopez
9. Correlative Light Atomic Force Electronic Microscopy (CLAFEM)
10. Atmospheric EM CLEM
11. Correlated Fluo Light SEM
12. Near infrared branding + SBF SEM
13. 4. Correlative MicroCT
Matthia Andrea Karreman
14. High-precision correlation
15. CLEM acquisition
Martin Schorb and Frank Sieckmann
16. ecCLEM mapping software
Xavier Heiligenstein and Perrine Paul-Gilloteaux
Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics.
Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.
- Contains contributions from experts in the field
- Covered topics include targeted ultramicrotomy and high-precision correlation
- Presents recent advances and currently applied correlative approaches
- Gives detailed protocols allowing the application of workflows in one’s own laboratory setting
- Covers CLEM approaches in the context of specific applications
- Aims to stimulate the use of new combinations of imaging modalities
Researchers and students in cell, molecular and developmental biology
- No. of pages:
- © Academic Press 2017
- 23rd May 2017
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Dr. Thomas Müller-Reichert is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in meiosis, mitosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in the early embryo of the nematode Caenorhabditis elegans and in tissue culture cells. He got his PhD degree from the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards to the EMBL in Heidelberg (Germany) for a post-doc with Dr. Tony Hyman. He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA) and set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is head of the Core Facility Cellular Imaging (CFCI) of the Medical Faculty Carl Gustav Carus of the TU Dresden (Germany).
Together with Dr. Paul Verkade he has developed a rapid transfer system for high-pressure freezing used for Correlative Light and Electron Microcopy. He has organized a number microscopy conferences and taught in several (CL)EM courses. He edited an MCB volume on the Electron Microscopy of Model Systems.
Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
Dr. Paul Verkade’s research focuses on the sorting mechanisms in intracellular transport pathways. His main tools are microscopy techniques, with an emphasis on electron microscopy (EM) in which field he has published over 50 papers. He has studied and got his PhD degree at the University of Utrecht, the Netherlands. After his post-doc time at the EMBL, Heidelberg, Germany in the group of Kai Simons and setting up a new EM lab at the Max Planck Institute for Molecular Cell Biology in Dresden, Germany he moved to the University of Bristol, UK in 2006. Here he set up a new EM unit as part of the Wolfson Bioimaging Facility, a fully integrated light and electron microscopy centre.
To support his transport studies, part of his research is to develop techniques and tools for the use of Correlative Light Electron Microscopy (CLEM). Amongst other things he has developed the Rapid Transfer System for the EMPACT2 high-pressure freezer together with Leica Microsystems. This allows for the combination of time-resolved CLEM with optimal preservation of ultrastructure for EM.
Dr. Verkade is chair of the Electron Microscopy section of the Royal Microscopical Society (RMS) and of the Cryo Microscopy Group, affiliated to the RMS. He has organised and taught on a large number of courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. He is also the principle organiser of the EMBO practical course on CLEM.
Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology & Pharmacology, University of Bristol, Bristol, UK