Correlative Light and Electron Microscopy III

Correlative Light and Electron Microscopy III

1st Edition - May 23, 2017

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  • Editors: Thomas Muller-Reichert, Paul Verkade
  • eBook ISBN: 9780128099766
  • Hardcover ISBN: 9780128099759

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Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics. Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.

Key Features

  • Contains contributions from experts in the field
  • Covered topics include targeted ultramicrotomy and high-precision correlation
  • Presents recent advances and currently applied correlative approaches
  • Gives detailed protocols allowing the application of workflows in one’s own laboratory setting
  • Covers CLEM approaches in the context of specific applications
  • Aims to stimulate the use of new combinations of imaging modalities


Researchers and students in cell, molecular and developmental biology

Table of Contents

  • 1. Millisecond time-resolved CLEM
    Gaia Pigino
    2. Super resolution LM und SEM of high-pressure frozen C. elegans
    Christian Stigloher
    3. Preservation fluorescence, super res CLEM
    Errin Johnson
    4. Targeted ultramicrotomy
    Yannick Schwab
    Yannick Schwab
    6. APEX in Tissue
    Robert G. Parton
    7. Corrsight mit IBIDI flowthrough chamber
    Miriam Susanna Lucas
    8. Corrsight
    Claudia S. Lopez
    9. Correlative Light Atomic Force Electronic Microscopy (CLAFEM)
    Frank Lafont
    10. Atmospheric EM CLEM
    Chikira Sato
    11. Correlated Fluo Light SEM
    Filip Braet
    12. Near infrared branding + SBF SEM
    Paul Verkade
    13. 4. Correlative MicroCT
    Matthia Andrea Karreman
    14. High-precision correlation
    Wanda Kukulski
    15. CLEM acquisition
    Martin Schorb and Frank Sieckmann
    16. ecCLEM mapping software
    Xavier Heiligenstein and Perrine Paul-Gilloteaux

Product details

  • No. of pages: 370
  • Language: English
  • Copyright: © Academic Press 2017
  • Published: May 23, 2017
  • Imprint: Academic Press
  • eBook ISBN: 9780128099766
  • Hardcover ISBN: 9780128099759

About the Serial Editors

Thomas Muller-Reichert

Thomas Muller-Reichert
Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM. TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019. He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy.

Affiliations and Expertise

Professor of Structural Cell Biology, Technische Universitat Dresden, TU Dresden, Germany

Paul Verkade

Paul Verkade
Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. His focus is on the study of sorting mechanisms in intracellular transport pathways and in the area of Synthetic Biology. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 85 papers and edited 4 books on CLEM (including 3 Volumes of the Methods in Cell Biology series). PV obtained his PhD on electron microscopic studies of the peripheral nervous system at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He has acted as chair and co-chair of the Electron Microscopy section of the Royal Microscopical Society and is closely involved with BioimagingUK shaping the UK imaging infrastructure landscape. He has organised and taught on several courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. His lab is the home of the EMBO practical course on CLEM and he is Work Group leader for CLEM within the EU COST project COMULIS (COrrelated MUltimodal imaging in LIfe Sciences).

Affiliations and Expertise

Professor of Bioimaging, University of Bristol, UK

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