Contamination in Tissue Culture

Contamination in Tissue Culture

1st Edition - January 1, 1973

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  • Editor: Jorgen Fogh
  • eBook ISBN: 9780323156981

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Contamination in Tissue Culture covers the sources, prevention, detection, and elimination of contamination in tissue culture. Composed of 12 chapters, the book describes the frequency of occurrence of contamination and the many different effects of contamination on cultured cells. After introducing the intraspecies contamination of cell cultures, the book explains a specific type of contamination, such as bacterial, fungal, viral, and parasitic contamination. A chapter in this book describes the reversible and irreversible alterations of cultured FL human amnion cells after experimental mycoplasmal infection. Chapters 9 and 10 examine the occurrence of tissue culture contaminants by electron microscopy and procedures for isolating and identifying viral contaminants. The concluding chapter covers sterility tests of media and solutions for tissue culture and the use of antibiotics. It also summarizes the major developments made as well as future challenges in the field. This book will be helpful to investigators, teachers, students, and technicians within the many disciplines of cell biology, physiology, cytology, virology, immunology, genetics, oncology, molecular biology, biochemistry, and biophysics, in which tissue and cell cultures are used, either as the primary object of research or as tools.

Table of Contents

  • List of Contributors


    Chapter 1. Extrinsic Cell Contamination of Tissue Culture

    I. Introduction

    II. Interspecies Identification of Cell Cultures

    III. Intraspecies Identification of Cell Cultures

    IV. Current Status of Cell Contamination


    Chapter 2. Methods of Prevention of Bacterial, Fungal, and Other Contaminations

    I. Source of Infection

    II. Procedures within the Transfer Room

    III. Laminar Flow Transfer Room

    IV. Aseptic Technique

    V. Antibiotics

    VI. Summary


    Chapter 3. Contamination of Tissue Culture by Bacteria and Fungi

    I. Introduction

    II. Materials and Methods

    III. Results

    IV. Discussion

    V. Summary


    Chapter 4. Contaminants Demonstrated by Microscopy of Living Tissue Cultures or of Fixed and

    Stained Tissue Culture Preparations

    I. Preparation of Cell Cultures for Microscopy

    II. Methods of Microscopy

    III. Photomicrographs Illustrating Various Types of Contaminants in Cell Cultures 69

    IV. Review of Data Obtained by Microscopy of Mycoplasma-Infected Cultures after Hypotonic Treatment and Orcein Staining


    Chapter 5. Contamination of Mammalian Cells in Culture by Mycoplasmata

    I. Introduction

    II. Biology of the Mycoplasmatales

    III. Epidemiology of the Contamination of Cell Cultures with Mycoplasmata

    IV. Host-Parasite Relationship of Mycoplasmata and Mammalian Cells

    V. Effect of Mycoplasmata on Cell Cultures

    VI. Elimination of Mycoplasmata from Cell Cultures

    VII. Conclusions


    Chapter 6. Mycoplasmal Contamination of Cell Cultures: Mycoplasma-Virus-Cell Culture Interactions

    I. Introduction

    II. Classification

    III. Detecting Cell Culture Contamination

    IV. Mycoplasmal Contamination

    V. Interactions between Mycoplasmas and Animal Cells

    VI. Mycoplasma-Virus-Cell Interactions


    Chapter 7. Reversible and Irreversible Alterations of Cultured FL Human Amnion Cells after

    Experimental Mycoplasmal Infection

    I. Effects of Mycoplasmal Infection on the in Vitro Growth of FL Cells

    II. In Vitro Growth and Susceptibility to Mycoplasmal Infection of Previously

    Mycoplasma-Infected Cells from which Mycoplasmas Have Been Eliminated

    III. Chromosome Changes following Mycoplasmal Infection of FL Cells

    IV. Reduced Tumor-Producing Capacity of Mycoplasma-Modified FL Cells

    V. Effects of Mycoplasmas on SV40 Transformation of Human Amnion Cells

    VI. Decreased Resistance of Transformed Human Cells to the Destructive Effects of Mycoplasmal Infection

    VII. Cell-Associated Mycoplasmas: Preferential Attachment to Transformed Cells and Cells of Malignant Origin

    VIII. Discussion of Results


    Chapter 8. Contamination in Tissue Culture by Parasites

    I. Introduction

    II. Identification of the Amoebae

    III. Source of Contamination

    IV. Methods of Detection, Prevention, and Cure

    V. Summary


    Chapter 9. Identification of Tissue Culture Contaminants by Electron Microscopy

    I. Introduction

    II. Examples of Tissue Culture Contaminations Observed under the Electron Microscope

    III. Normal Particulate Cell Organelles of Viral Size

    IV. Abnormal Cell Structures of Possible Viral Significance

    V. Methods of Electron Microscopic Identification of Viral Contaminants


    Chapter 10. Endogenous Virus Contaminants in Fetal Bovine Serum and Their Role in Tissue

    Culture Contamination

    I. Introduction

    II. Target Viruses

    III. Procedures for Isolating and Identifying Viral Contaminants

    IV. Results

    V. Discussion and Conclusions


    Chapter 11. The Presence of Viruses in Uninoculated Tissue Cultures: Sources and Methods of


    I. Introduction

    II. Passenger, Indigenous, and Cryptic Viruses

    III. The Relationship of Viruses to an Ability of Cells to Grow in Culture

    IV. Significance of the Presence of Viruses Inherent to Cultured Cells

    V. Detection of Viruses Indigenous to Cultured Cells


    Chapter 12. Summary

    I. Introduction

    II. Types and Sources of Contamination

    III. Sterility Tests of Media and Solutions

    IV. Detection and Identification of Contaminants

    V. Prevention of Contamination

    VI. Treatment of Contaminated Cultures

    VII. Use of Antibiotics

    VIII. Effects of Contaminants on Cell Cultures

    IX. Concluding Remarks


    Author Index

    Subject Index

Product details

  • No. of pages: 300
  • Language: English
  • Copyright: © Academic Press 1973
  • Published: January 1, 1973
  • Imprint: Academic Press
  • eBook ISBN: 9780323156981

About the Editor

Jorgen Fogh

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