This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.

Key Features

A. Expert authors who are leaders in the field

B. Extensively referenced and useful figures and tables

C. Provides hints and tricks to facilitate reproduction of methods


Researchers and students in cell, molecular and developmental biology

Table of Contents

    1. Identification and characterization of Steroidogenic Factor-1 inverse agonists.
    2. Mabrouka Doghman, Franck Madoux, Peter Hodder, and Enzo Lalli

    3. Assessment of inverse agonism for the angiotensin II type 1 receptor
    4. Hiroshi Akazawa, Noritaka Yasuda, Shin-ichiro Miura, Issei Komuro

    5. Measurement of inverse agonism in BARs
    6. Carlos Alberto Taira

    7. Inverse agonism of antidepressants: in vitro and in vivo studies
    8. Joel Bockaert

    9. Differential inverse agonism at the human muscarinic M3 receptor
    10. Paola Casarosa

    11. Ghrelin Receptor: High Constitutive Activity and Methods for Developing Inverse Agonists.
    12. Constance Chollet

    13. Constitutive activity and inverse agonism at the α1a and α1b adrenergic receptor subtypes.
    14. Susanna. Cotecchia

    15. Measurement of inverse agonism of the cannabinoid receptor
    16. Tung Fong

    17. Constitutively Active Thyrotropin (TSH) and Thyrotropin-Releasing Hormone (TRH) Receptors and Their Inverse Agonists
    18. Marvin C. Gershengorn

    19. Inverse retinoid agonists and neutral antagonists
    20. Hinrich Gronemeyer

    21. γ-Aminobutyric Acid Type A (GABAA) Receptor Subtype Inverse Agonists as Therapeutic Agents in Cognition
    22. Gabriella Guerrini and Ciciani Giovanna

    23. Assays for inverse agonists in the visual system.
    24. Masahiro Kono

    25. Receptor-driven identification of novel human A3 adenosine receptor antagonists as potential therapeutic agents
    26. Silvia Paoletta, Stephanie Federico, Giampiero Spalluto and Stefano Moro

    27. Inverse agonists of steroidogenic factor SF-1
    28. Fabrice Piu and Andria L. Del Tredici

    29. Methods to Measure G Protein Coupled Receptor Activit


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