Constitutive Activity in Receptors and Other Proteins, Part A

Constitutive Activity in Receptors and Other Proteins, Part A

1st Edition - November 11, 2010

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  • Editor: Michael Conn
  • eBook ISBN: 9780123812995
  • Hardcover ISBN: 9780123812988

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This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.

Key Features

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods


Researchers and students in cell, molecular and developmental biology

Table of Contents

  • Section I-- Identification and Measurement of Constitutive Activity

    1. Constitutive activity at the cannabinoid CB1 receptor and behavioral responses
    2. Katherine E. Hanlon and Todd W. Vanderah

    3. Detecting constitutive activity and protean agonism at the cannabinoid-2 receptor.
    4. Massimiliano Beltramo, Rossella Brusa, Isabella Mancini, Paola Scandroglio, Massimiliano. Beltramo

    5. Modulation of constitutive activity of the ghrelin receptor by use of pharmacological tools as well as mutagenesis
    6. Birgitte Holst and Jacek Mokrosiński

    7. Assessment of constitutive activity and internalization of GPR54 (Kiss1-r).
    8. Macarena Pampillo and Andy Videsh Babwah

    9. Assessment of Constitutive Activity in EP Prostanoid Receptors
    10. Hiromichi Fujino, Toshihiko Murayama, and John W. Regan

    11. α1D-Adrenergic receptors: constitutive activity and reduced expression at the plasma membrane
    12. J Adolfo Garcia-Sáinz, M. Teresa Romero-Ávila and Luz del Carmen Medina

    13. Constitutive activity of the histamine H1 receptor
    14. Saskia Nijmeijer, Rob Leurs and Henry F Vischer

    15. Constitutive activity of somatostatin receptor subtypes
    16. Anat Ben-Shlomo, Kolja Wawrowsky and Shlomo Melmed

    17. Assessment of homologous internalization of constitutively active N111G mutant of AT1 receptor
    18. Mohiuddin Ahmed Bhuiyan and Takafumi Nagatomo

    19. Methods to detect cell surface expression and constitutive activity of GPR6
    20. Balakrishna M. Prasad, Bettye Hollins and Nevin A. Lambert

    21. β3-adrenoceptor agonists and (antagonists as) inverse agonists: history, perspective, constitutive activity and stereospecific binding.
    22. Maria Grazia Perrone and Antonio Scilimati

    23. Constitutive Activity of the Lutropin Receptor and its Allosteric Modulation by Receptor Heterodimerization
    24. Deborah L. Segaloff

    25. Assessing Constitutive Activity of Extracellular Calcium-Sensing Receptors in Vitro and in Bone
    26. Wenhan Chang, Melita Dvorak, and Dolores Shoback

    27. Constitutive Activity of Neural Melanocortin Receptors.
    28. Ya-Xiong Tao, Hui Huang, Zhi-Qiang Wang, Fan Yang, Jessica N. Williams, and Gregory V. Nikiforovich

    29. Measurement of Constitutive Activity of BMP Type I receptors
    30. Peter ten Dijke

    31. Probing the CA among dopamine D1 and D5 receptors and their mutants
    32. Bianca Plouffe, Jean-Philippe D’Aoust, Vincent Laquerre, Binhui Liang and Mario Tiberi

    33. Identification of Gain-of-Function Variants of the Human Prolactin Receptor
    34. Vincent Goffin, Roman L. Bogorad, and Philippe Touraine

    35. Investigations of Activated ACVR1/ALK2, a Bone Morphogenetic Protein (BMP) Type I Receptor that Causes Fibrodysplasia Ossificans Progressiva
    36. Frederick. Kaplan, Petra Seemann, Julia Haupt, Meiqi Xu, Vitali Lounev, Mary Mullins, Eileen M. Shore

    37. Identification and evaluation of constitutively active thyroid stimulating hormone receptor mutations
    38. Joaquin Lado-Abeal, Leah R. Quisenberry, and Isabel Castro-Piedras

    39. Assessment of constitutive activity of a G protein-coupled receptor, Cpr2, in Cryptococcus neoformans by heterologous and homologous methods
    40. Chaoyang Xue, Yina Wang, and Yen-Ping Hsueh

    41. In vitro and in vivo assessment of mu opioid receptor constitutive activity.
    42. Edward J. Bilsky, Denise Giuvelis, Melissa D. Osborn, Christina M. Dersch, Heng Xu and Richard B. Rothman

    43. Constitutively active µ-opioid methods
    44. Mark. Connor and John Traynor

    45. Protein Kinase Ck2 is a Constitutively-Active Enzyme That Promotes Cell Survival: Strategies To Identify Ck2 Substrates and Manipulate Its Activity In Mammalian Cells
    46. Jacob P. Turowec, James S. Duncan, Ashley C. French, Laszlo Gyenis, Nicole A. St. Denis, Greg J. Vilk, and David W. Litchfield

    47. Assessment of Ck2 Constitutive Activity In Cancer Cells
    48. Maria Ruzzene, Giovanni Di Maira, Kendra Tosoni, and Lorenzo A. Pinna

    49. Structural basis of the constitutive activity of protein kinase CK2
    50. Birgitte B. Olsen, Barbara Guerra, Karsten Niefind and Olar-Georg Issinger font error

    51. Measuring the Constitutive Activation of c-Jun N-terminal Kinase Isoforms
    52. Ryan T. Nitta, Shawn S. Badal, and Albert J. Wong

    53. Measurement of constitutive MAPK and PI3K/AKT signaling activity in human cancer cell lines.
    54. Keiran S.M. Smalley

    55. Constitutive activity of GPR40/FFA1: Intrinsic or assay dependent?
    56. Leigh A Stoddart and Graeme Milligan

    57. Constitutive activity of TRP channels: methods for measuring the activity and its outcome
    58. Baruch Minke

    59. Measurement of Orexin (Hypocretin) and Substance P Effects On Constitutively Active Inward Rectifier K+ Channels in Brain Neurons
    60. Yasuko Nakajima and Shigehiro Nakajima

    61. Characterization of G protein receptor kinase 4 and measuring its constitutive activity in vivo
    62. Bradley T. Andresen

    63. Voltage-clamp based methods for the detection of constitutively active acetylcholine-gated IK,ACh channels in the diseased heart
    64. Niels Voigt, Samy Makary, Stanley Nattel, Dobromir Dobrev

    65. Assaying Wave and WASH complex constitutive activities towards the Arp2/3 complex.

    Emmanuel DERIVERY and Alexis GAUTREAU

Product details

  • No. of pages: 804
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: November 11, 2010
  • Imprint: Academic Press
  • eBook ISBN: 9780123812995
  • Hardcover ISBN: 9780123812988

About the Serial Volume Editor

Michael Conn

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

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